96. Jahrestagung der Deutschen Gesellschaft für Pathologie e. V ...

Mechanismen, die eine Progression des DCIS zum invasiven duktalen
Mammakarzinom begünstigen.
SA-P-018
Elevated expression of LSD1 (Lysin-specific demethylase 1) during
tumour progression from pre-invasive to invasive ductal carcinoma
of the breast
N . Bektas Serce1 , A . Gnatzy1 , S . Steiner 1 , J . Kirfel1 , R . Büttner2 1 2 University of Bonn, Institute of Pathology, Bonn, University of Cologne,
Institute of Pathology, Köln
Aims. Lysin-specific demethylase 1 (LSD1) is a nuclear protein which
belongs to the aminooxidase enzymes playing an important role in
controlling gene expression. It has also been found highly expressed in
several human malignancies including breast carcinoma. Our aim was
to detect LSD1 expression also in pre-invasive neoplasias of the breast.
In the current study we therefore analyzed LSD1 protein expression in
ductal carcinoma in situ (DCIS) in comparison to invasive ductal breast
cancer (IDC).
Methods. Using immunhistochemistry we systematically analyzed LSD1
expression in low grade DCIS (n=27), intermediate grade DCIS (n=30),
high grade DCIS (n=31) and in invasive ductal breast carcinoma (n=32).
SPSS version 18.0 was used for statistical analysis.
Results. LSD1 was differentially expressed in DCIS and invasive ductal
breast cancer. Interestingly, LSD1 was significantly overexpressed in
high grade DCIS versus low grade DCIS. Differences in LSD1 expression
levels were also statistically significant between low/intermediate DCIS
and invasive ductal breast carcinoma.
Conclusions. LSD1 is also expressed in pre-invasive neoplasias of the breast.
Additionally, there is a gradual increase of LSD1 expression within
tumour progression from pre-invasive DCIS to invasive ductal breast
carcinoma. Therefore upregulation of LSD1 may be an early tumour promoting
event.
SA-P-019
Female adnexal tumor of probable Wolffian origin – case report
and review of literature for epidemiology, course of disease and
differential diagnosis
K . Friedrich1 , M . Toma 1 , J . Wienold2 , G . Baretton1 1University Hospital “Carl Gustav Carus” Dresden, Institute of Pathology,
Dresden, 2Hospital Weißeritztal Kliniken Freital Dippoldiswalde, Deparment
of Gynecology and Obstetrics, Freital
Aims. Clinical history: 35-year-old female with lower abdominal pain on
laparoscopy showed a hematosalpinx and a tumor close to the right fallopian
tube, which was removed laparoscopically. The following laparoscopic
staging did not revealed any further tumors.
Methods. Pathology: Gross examination showed a tumor (7 cm diameter)
with close contact to the fallopian tube, partially covered by serosa
with nodular and focally light gray, glistening pale yellow sectioned surface.
Microscopically, the tumor showed variable histological architecture
with solid, tubular and sieve-like pattern formed by low cuboidal,
attenuated or spindle-shaped cells without atypia and only few mitoses.
The tumor cells expressed cytokeratin 8/18 and 19, CD10, CD99, vimentin
and – at least focally – inhibin and calretinin. EMA, estrogen-, progesterone
receptor, cytokeratin 7 and 20, CD34 and S100 were not detectable.
Based on the histomorphology and the immunohistochemical
expression profile, a female adnexal tumor of probable Wolffian origin
(FATWO) was diagnosed.
Results. Epidemiology, course of disease and differential diagnosis: FAT-
WO are very rare tumors of female adnexal region. A total of 72 cases
have been thus far documented in the literature. The age of reported
patients ranged from 15 to 83 years, most patients are between 40 and
45 years old. Remnants of the Wolffian duct, especially the rete ovarii,
are thought to be the origin of this tumor. The broad ligament is the most
frequent location, but the tumor may also occur in the serosa of the fallopian
tube, the ovary, the mesoalpinx, retroperitoneum and peritoneum.
Conclusions. Most cases are benign, but ten of the reported patients developed
local recurrences, lung or liver metastases. Three patients died
of their tumor. Atypia and a high proliferation activity may predict an
aggressive behaviour. But tumors without these criteria showed recurrences,
too. The differential diagnoses are sex-cord stroma tumors (Sertoli-Leydig
cell tumors, granulosa cell tumors), endometroid adenocarcinomas
(of the fallopian tube), adenomatoid tumors, mesotheliomas
and metastases.
Poster: Gynäkopathologie und Mammapathologie II
SA-P-020
T lymphocytic infiltration in serous ovarian carcinoma:
expression and survival analysis
S . Scheil-Bertram1 , H .-C . Bösmüller2 , A . du Bois3 , F . Heitz3 , P . Harter3 ,
M . Oppitz1 , N . Ewald-Riegler4 , R . Hils4 , A . Fisseler-Eckhoff 1
1 2 Institute of Pathology &Cytology, Wiesbaden, Institute of Pathology, Linz,
Austria, 3Dept Gynecology & Gyn . Oncology, Essen, 4Clinic for Gynecology &
Gyn . Oncology, Wiesbaden
Aims. The lymphocytic infiltration of carcinomas should be a prognostic
marker of antitumoral immunoreactivity and be associated with prolonged
overall survival in ovarian carcinomas. Using CD3 and CD8 antibodies,
we analysed immunreactive T-lymphocyts in a cohort of 78 serous
ovarian carcinoma (OC), and their correlation with patients’ survival.
Methods. We used the CD3 (clone SP7) and CD8 (clone C8/144B) antibodies
(both NeoMarkers). Immunohistochemistry was performed on
multi tissue microarrays (78 patients; median age at diagnosis 64 years).
The stromal and intraepithelial T-cell (CD3 and CD8) density was
quantified, counting the average number of cells per high power field
(HPF=400x) and reviewing a total of 10 HPF for each microarray. The
immunoreactivity was analyzed in a double blind fashion by two pathologists.
Results. The survival of patients with a CD3/CD8 ratio above 1 or 2 respectively
was significantly shorter than with a CD3/CD8 ratio below 1
(17.3 or 15.1 month versus 22.2 month; p=0.0154; hazard ratio: