96. Jahrestagung der Deutschen Gesellschaft für Pathologie e. V ...

More documents

Recommendations

Info

ned, if well-defined mantle zones, which can be identified in a subset of FL including FL “in situ”, are part of the malignant clone. Methods. FL cases with morphologically detectable mantle zone structures and a case of follicular lymphoma “in situ” were selected from a series of 240 FL grade 1/2. Fluorescence in situ hybridization (FISH) for the detection of the chromosomal translocation t(14;18)(q32;q21) was combined with a simultaneous immunofluorescence staining for IgD for the identification of mantle zone cells (FICTION). Results. 10 of 17 (59%) FL cases with morphological detectable mantle zone structures lacked a t(14;18) by FISH. In the remaining 7 t(14;18) positive FL cases and the FLIS case, the IgD+ mantle zone cells did not show a chromosomal break in the BCL2 gene locus. Conclusions. 1) A significant part of FL cases with prominent mantle zone structures are t(14;18) negative. Further investigations are necessary to elucidate the molecular background of this subgroup and to define the border with nodal marginal zone lymphoma with follicular colonization. 2) The mantle zone cells in t(14;18)+ FL with prominent mantle zones in their majority are not part of the malignant clone and therefore most likely represent either pre-existent or newly recruited reactive cells. DO-050 Reactive tumor infiltrating T-cells predict survival in mantle cell lymphoma: an immunohistochemical study of 81 patients C . Schrader1 , Ö . Akalthun1 , P . Meusers2 , G . Brittinger2 , J . Claasen1 , W . Klapper3 1 2 2 University Hospital of Kiel, nd Department of Medicine, Kiel, University Essen, Department of Hämatology, 3UKSH, Campus Kiel, Department of Pathology Aims. The role of tumor infiltrating T-Cells in malignant B-Cell lymphomas is discussed controversial. There are only limited data on CD 8 and FOXP3 positive cells in mantle cell lymphoma. Methods. 81 biopsy specimens of patients (64 men and 17 women) with mantle cell lymphoma and a median age of 64 years (range: 41 to 86 years) were included in this study. The slides were stained immunohistochemically with CD3, CD8 and FOXP3. Positive T-cells of 10 High power fields (HPF) were counted and the average value was calculated. Results. The CD 8 staining showed a range of 0 to 138 positive cells per HPF with a mean value of 19.4/HPF. A high account of CD 8 positive cells was associated with a significantly longer overall survival time (42 months) compared to MCL with a low account of CD 8 positive cells (28.8 months, p=0.029). FOXP3 staining had a range of 0 to 104/HPF with a mean value of 28. Patients with MCL and a high number (>25/ HPF) of FOXP3 positive cells had a median survival time of 38.2 months compared with the group with low account (
Abstracts DO-053 Account of tumor infiltrating macrophages is a prognostic factor for patients with mantle cell lymphoma C . Schrader 1 , F . Sirin 1 , P . Meusers 2 , G . Brittinger 2 , J . Claasen 1 , W . Klapper 3 1 University Hospital of Kiel, 2 nd Department of Medicine, Kiel, 2 University Essen, Department of Hämatology, 3 UKSH, Campus Kiel, Department of Pathology Aims. Mantle cell lymphoma (MCL) is a malignant lymphoma associated with a relatively aggressive clinical course and a median overall survival time of 3–4 years. Only limited data about tumor associated macrophages and their influence on survival in MCL exists. Methods. We analyzed the amount of CD68 macrophages in relation to the clinical outcome in patients with MCL. Lymph node biopsies of 77 untreated patients (17 women and 60 men) enrolled in two multicenter trials (1975–1985) with a median age of 66 years (range 41–86 years) were included in this study. Biopsy specimens were investigated immunohistochemically with monoclonal antibodies against CD68 (Ki-M1P). 10 High power fields (HPF) were evaluated by random. Results. Patients with low account (less than 10/HPF) of CD 68 positive macrophages had a median overall survival time of 38.2 months, compared to 24.2 months for patients with high (more 10/HPF) CD 68 positive macrophages. The Kaplan-Meier analysis showed a significant difference in the overall survival time (p=0.0027). Conclusions. Patients with mantle cell lymphoma and a low number of CD 68 positive macrophages have a better prognosis and can predict outcome. DO-054 Transformation of gastritis to gastric marginal zone lymphoma is associated with deregulated expression of microRNAs C . Thorns1 , J . Kuba1 , A .C . Feller1 , V . Bernard 1 , A . Senft2 , S . Szymczak2 , H .-W . Bernd1 1 2 UKSH, Campus Lübeck, Pathology, University Lübeck, Institute for medical bioinformatics and statistics Aims. Gastric extranodal marginal zone lymphoma (MALT lymphoma) generally evolve from a chronic Helicobacter pylori-positive gastritis. The mechanisms that promote the malignant transformation from gastritis to lymphoma are not well understood. This study aims to identify microRNAs that might be involved in the process of neoplastic transformation. Methods. Gastric biopsies were scored as 0 (normal), 1 (gastritis), 2 (follicular gastritis), 3 (suspicious, probably reactive), 4 (suspicious, probably lymphoma) and 5 (MALT lymphoma) (Wotherspoon scores). Groups 3, 4, and 5 were further evaluated for monoclonality by immunohistochemistry for immunoglobulin light chains and/or by polymerase-chainreaction for the immunoglobulin heavy chain locus (IgH). MicroRNAsignatures of 68 cases were generated by RT-PCR for 376 miRNAs. Results. MicroRNA signatures revealed a total of 41 miRNAs that were significantly upregulated (n=33) or down regulated (n=8) in succession from normal mucosa to gastritis and to MALT-lymphoma. Some of these reflect the normal expression in lymhocytes (e.g. miR-566 and -212), while others are known to be the effect of Helicobacter pylori infection (e.g. miR-155 and let7f). A group of five miRNAs (miR-150, -550, -124a, -518b and -539) were differentially expressed in gastritis (Wotherspoon scores 1, 2 and polyclonal 3 and 4) and lymphomas (monoclonal scores 3, 4, and 5). These are likely to be involved in the malignant transformation of gastritis into MALT lymphoma. Conclusions. The development of gastric MALT-lymphoma out of chronic gastritis is paralleled by the deregulation of distinct microRNAs which might thus be centrally involved in the process of malignant transformation. 28 | Der Pathologe · Supplement 1 · 2012 AG Hämatopathologie II DO-055 Bcl6 expression is not limited to germinal center B-cells and is associated with progression of extranodal marginal zone B-cell lymphomas of the gastrointestinal tract U . Boruschek1 , L . Floßbach1 , M . Buck1 , S . Brüderlein1 , P . Möller1 , T .F .E . Barth1 1Ulm University, Pathology, Ulm Aims. We have previously shown that progression in gastrointestinal B-cell lymphomas is associated with changes in the BCL6 locus and with an increased expression of Bcl6 [Flossbach et al. Int J Cancer 2011; 129(1):70–7]. Bcl6 is a well known germinal center marker; the extranodal marginal zone B-cell lymphomas (MALT lymphomas) are supposed to stem from cells of the extrafollicular space, therefore the expression of Bcl6 in these lymphomas during progression seems conflicting. We have analyzed the detailed expression of Bcl6 in lymph nodes with toxoplasmosis since one of the cells discussed to be the potential progenitor of MALT lymphomas is the monocytoid B-cell. Methods. We studied lymph node paraffin sections of four patients with toxoplasmosis by double immunofluorescence staining using a broad panel of antibodies. For this purpose we first established a new technique based on sequential heating of the samples that now even allows the application of antibodies from the same species or the simultaneous use of monoclonal and polyclonal antibodies. Further we performed stimulation experiments with lymphoblastoid B-cells. Results. We found a strong expression of Bcl6 in the germinal center. However, we also detected some scattered Bcl6 positive cells in the extrafollicular space. These extrafollicular Bcl6-positive cells were characterized as: CD19+,CD75+, AID+, IgA+, IgG+/−, CD30−, CD10−, CD38−, Bcl2−, ZAP70−, cRel−, IgM−, IgD−, CD11c−. Monocytoid B-cells expressed Bcl6 in a subfraction of less than 1%. The profile of the Bcl6+ extrafollicular B-cells corresponds to an activated post germinal center B-cell differing from monocytoid B-cells. Expression of Bcl6 was partially inducible in lymphoblastoid EBV transformed B-cells by TNFα, TPA, and LPS. Conclusions. We conclude that Bcl6 expression is not limited to germinal B-cells but is also found in extrafollicular B-cells. We suggest that the blastic variant of MALT lymphoma may have preserved the potential of up-regulating Bcl6 as an antiapoptotic mechanism. DO-056 Characterization of gastrointestinal marginal zone B-cell lymphoma and large cell variants using high-resolution SNP-arrays L . Floßbach1 , K . Holzmann2 , T . Mattfeldt 1 , P . Möller1 , T .F .E . Barth1 1 2 Ulm University, Pathology, Ulm, Ulm University, IZKF, Ulm Aims. Gastrointestinal marginal zone B-cell lymphomas (MALT Lymphomas) are a model for tumor progression since we and others have shown that the frequently coexisting more aggressive large cell component is clonally related to the small cell lymphoma. We used SNP analysis to further characterize these lymphomas. Methods. We extracted genomic DNA from frozen tissue samples of 28 gastrointestinal marginal zone B-cell lymphomas (n=7) and large cell variants (n=21). We performed SNP analysis using the Affymetrix HGW SNP array 6.0 platform. Results were correlated with FISH and IHC analyses. Results. While small cell lymphomas have on the average 8 aberrations longer than 0.2MB each case, large cell variants have more than 14. Both small and large cell lymphomas have losses in regions 1p13 and 6q15 as well as gains on 1p36 and 17q21. Gains on 9q12i-j (2/7) are restricted to small cell lymphomas. Losses on 6q24 (5/21) and gains on 11q23 (8/21) are restricted to the large cell lymphomas. Most frequent losses or deletions concern region 6q14.1a-c containing HTR1B, IRAK1BP1, PHIP, HMGN3, LCA5 and SH3BGRL2 (5/21 large cell and 1/7 small cell lym-
Page 2 and 3: Inhalt Der Pathologe · Supplement
Page 4 and 5: Inhalt Der Pathologe · Supplement
Page 6 and 7: Editorial Liebe Kolleginnen und Kol
Page 8 and 9: Kolorektales Karzinom 2 VO-005 Tran
Page 10 and 11: Keynote Lecture VO-014 Genetic dete
Page 12 and 13: (AMACR, FASN, GOLM1, GSP-pi, ERG) t
Page 14 and 15: to assess the correct rate of R1 re
Page 16 and 17: DNA damage, and cytotoxic drugs. Au
Page 18 and 19: HCV-positive formalin-fixed and par
Page 20 and 21: well as MET activation were examine
Page 22 and 23: or BRAF mutation, c-MYC and SIRT1 e
Page 24 and 25: AG Pneumopathologie III DO-032 Remo
Page 26 and 27: immature granulopoiesis showed a st
Page 30 and 31: phomas). Most prominent gains or am
Page 32 and 33: DO-062 Tumor-associated macrophages
Page 34 and 35: DO-080 DOG1: an immunohistochemical
Page 36 and 37: AG Oralpathologie DO-087 Detection
Page 38 and 39: DO-094 Do activated fibroblasts inf
Page 40 and 41: DO-101 Histopathological analysis o
Page 42 and 43: DO-108 Identification of potential
Page 44 and 45: Conclusions. In conclusion, 454 par
Page 46 and 47: subgroup of patients with B-Raf mut
Page 48 and 49: DO-002b Impact of terminologies in
Page 50 and 51: Methods. We performed MCPyV-FISH of
Page 52 and 53: FR-013 HPV-genotype distribution in
Page 54 and 55: Methods. Three different techniques
Page 56 and 57: (i.e. investment in equipment and e
Page 58 and 59: FR-032 COLD-PCR: a powerful tool in
Page 60 and 61: dissection (MBLND) technique to imp
Page 62 and 63: SO-005 Prevalence of mutations in s
Page 64 and 65: SO-011 Methylation profiling and in
Page 66 and 67: more effective than SAHA alone and
Page 68 and 69: SO-022 Specialized pathology review
Page 70 and 71: SO-027 Ki-67 in mitotic score group
Page 72 and 73: nificantly associated with advanced
Page 74 and 75: liver did not correlate with the mu
Page 76 and 77: AG Paidopathologie II SO-046 Overex
Page 78 and 79:
Results. Histomorphology of the ova
Page 80 and 81:
p42 and p27 have not yet been inves
Page 82 and 83:
SO-068 Recent advances in understan
Page 84 and 85:
SO-075 A novel, dual role of CCN3 i
Page 86 and 87:
Pancreatic Adenocarcinoma SG-137 Se
Page 88 and 89:
sease and 30 colon cancer serum sam
Page 90 and 91:
Conclusions. Although CRC is charac
Page 92 and 93:
differentiated and TNM stage III or
Page 94 and 95:
pressed moderate levels of the prot
Page 96 and 97:
FR-P-037 MALDI imaging reveals COX7
Page 98 and 99:
in the context of therapy response
Page 100 and 101:
on primary cells of wild-type and c
Page 102 and 103:
chemotherapy was recommended and co
Page 104 and 105:
aims are twofold: 1) to verify the
Page 106 and 107:
Results. Her2/neu status in TMAs an
Page 108 and 109:
prognostic impact was found in rect
Page 110 and 111:
provided information on survival ti
Page 112 and 113:
Methods. Biopsy specimens from 430
Page 114 and 115:
the OS rate was 53% for patients wi
Page 116 and 117:
FR-P-098 Immunohistological stainin
Page 118 and 119:
FR-P-104 Histopathological evaluati
Page 120 and 121:
within the earliest morphologic det
Page 122 and 123:
Conclusions. In primary imaging a g
Page 124 and 125:
fibrotic neointma development as we
Page 126 and 127:
FR-P-129 Alpha-1-antitrypsin-PiZ-an
Page 128 and 129:
FR-P-136 The expression of central
Page 130 and 131:
cosa and in the periarterial tissue
Page 132 and 133:
FR-P-149 Combination of Castleman d
Page 134 and 135:
or without different concentrations
Page 136 and 137:
Results. In 18 cases (79%) the caus
Page 138 and 139:
FR-P-168 Autopsy findings in a 2-ye
Page 140 and 141:
FR-P-174 MPGN-like glomerulonephrit
Page 142 and 143:
Poster: Gynäkopathologie und Mamma
Page 144 and 145:
SA-P-011 Genetic aberrations of pre
Page 146 and 147:
Mechanismen, die eine Progression d
Page 148 and 149:
internet server. A network of inter
Page 150 and 151:
Methods. HE-gefärbte Schnittpräpa
Page 152 and 153:
Conclusions. The newly released 450
Page 154 and 155:
and desmoplastic reaction are diffe
Page 156 and 157:
one patient revealed more than 9 mi
Page 158 and 159:
situation, the V600E mutation in th
Page 160 and 161:
SA-P-064 Evolution of molecular pat
Page 162 and 163:
nic markers such as myf4 and MyoD1
Page 164 and 165:
Conclusions. To our knowledge, this
Page 166 and 167:
SA-P-085 Expression of the eukaryot
Page 168 and 169:
Results. The papillary RCC type II
Page 170 and 171:
SA-P-098 Male infertility: assessme
Page 172 and 173:
SA-P-104 Process oriented scientifi
Page 174 and 175:
Conclusions. The IBDW offers a uniq
Page 176 and 177:
L Lasitschka F. DO-046 Lehmann A. F
show all

96. Jahrestagung der Deutschen Gesellschaft für Pathologie e. V ...

Create successful ePaper yourself

Delete template?

Save as template?