96. Jahrestagung der Deutschen Gesellschaft für Pathologie e. V ...

More documents

Recommendations

Info

FR-P-149 Combination of Castleman disease and Langerhans cell histiocytosis in a supraclavicular lymph node H . Geddert 1 , A . Dimmler 1 , U . Götschin 2 , M . Schatz 3 , G . Faller 1 1 St . Vincent Hospital, Institute of Pathology, Karlsruhe, 2 St . Vincent Hospital, Department of General Surgery, Karlsruhe, 3 St . Vincent Hospital, Department of Hematology and Oncology, Karlsruhe Aims. A 51-year-old female patient presented to our hospital with a 5 cm measuring mass in the right supraclavicular fossa. Because of known nodular goiter an ectopic thyroid nodule was suspected. The patient was in good clinical condition without B symptoms. To exclude malignant lymphoma the whole mass was excised. Methods. During intraoperative frozen section suspicion of Castleman disease was raised, although a second cellular component remained unclear. Results. After routine tissue processing diagnosis of Castleman’s disease of hyaline-vascular subtype in a lymph node was confirmed. The second component emerged as Langerhans cell histiocytosis. No signs of multicentric Castleman disease or its associated conditions were found. Systemic Langerhans cell histiocytosis was excluded clinically. Conclusions. One year after diagnosis the patient is still in good condition without any sign of relapse. To our knowledge, this is the first report of a combination of Castleman disease and Langerhans cell histiocytosis in a single lymph node. FR-P-150 Notch2, possible a crucial gene in pathogenesis in lymphoma X .-x . Zhang1 , Q . Lai1 , R . Zhou1 1Zhejiang University, School of Medicine, Hangzhou, China Aims. In mammals, there are four kinds of Notch transmembrane receptors (Notch1 to 4); and there are many kinds of Notch target genes include Hes family, nuclear factor kappaB (NF-κB), cyclin D1, c-myc, p21, p27, etc. In recent, researches show that Notch2 signaling may be an important regulator of B cell lymphocytes activation and effect cell differentiation. However, the functions of Notch2 in lymphoma remain poorly understood. In our study, we try to investigate the role which Notch2 plays in the pathogenesis of lymphoma, especially on B cell lymphoma. Methods. First, Notch2 was detected in 95 B cell lymphoma samples through PCR, and DNA sequencing was used to see if there were mutations or not. Then the pEYFP-n1, which contains the Notch2 gene or not, was transfected into the lymphoma cell lines (include raji, ramos, daudi, jurkat and molt4) and 293T cell by electro-transfection. And then total RNA and protein were extracted from the transfected cells, and then the expression levels of targeted genes, such as c-myc, cyclinD1, p21, p27, bcl6, p50 and p65, were detected by real-time PCR or Western blot. Statistical analysis was done by the Student’s t-test between the test groups and the control group. A P-value of
Abstracts nal lymph nodes and splenomegaly. Investigation for TCR gamma gene rearrangement revealed the presence of a dominant amplificate. After 8 cycles of standard cytotoxic chemotherapy according to the CHOP regimen a complete remission was achieved. Eleven years later, the patient presented with cervical and axillary lymphadenopathy and splenomegaly. Histological and immunohistological examination of an excised lymph node revealed a T-cell lymphoma with the characteristic features of AITL. Clonality analysis for TCR gamma chain genes revealed a clonal population with a different amplificate size that those from the initial manifestation. Conclusions. To our knowledge, this is the longest recurrence free period in AITL reported so far. Most interestingly the patient exhibited a novel T-cell clone at relapse not identifiable even among the minor clones at initial presentation. FR-P-153 Severe central nervous system (CNS) graft versus host disease (GVHD) in a patient without any other GvHD symptoms after allogeneic stem cell transplantation C . Schrader1 , R . Stingele2 , W . Brück3 , I . Metz3 , C . Riedel4 , N . Schub1 , A . Humpe1 , T . Valerius1 , G . Deuschel2 , M . Gramatzki1 , A . Günther1 1 2 2 University Hospital of Kiel, nd Department of Medicine, Kiel, UKSH, Campus Kiel, Department of Neurology, 3University of Göttingen, Department of Pathology, 4UKSH, Campus Kiel, Department of Neuroradiology Aims. Although graft versus horst disease (GvHD) is the most relevant complication of allogeneic stem cell transplantation (SCT), it rarely affects the central nervous system. Recently, a consensus conference proposed criteria of diagnosis for cerebral GvHD including the obligatory manifestation of chronic GvHD at other organs [Grauer et al., Brain, 133: 2852, 2010]. We observed a 41-year-old women, who developed spastic paralysis and fell into coma 2.5 years after having an allogeneic peripheral blood stem cell stem cell transplantation (PBSCT) for acute myeloblastic leukemia from an unrelated HLA 9/10-matched donor. The patient presented with a history of several month of headache supposed to be caused by migraine. She had a history of acute GvHD stage III (skin and intestinal) but no signs of chronic GvHD. In addition she had no history of an independent autoimmunopathy or migraine prior to SCT. Methods. MRI scan was performed, cerebrospinal fluid was analyzed to exclude CNS relapse and infectious agents, and finally CNS biopsy was obtained by open brain surgery. Results. MRI scan showed disseminated severe leucencephalopathy without established sign of CNS relapse, lymphoma or typical infection. The cerebrospinal fluid analysis was normal. Toxoplasmosis and viral infection such as HSV, VZV, ADV, EBV, cmV, HHV-6 and HIV was excluded by PCR. The blood lymphocyte subset showed lymphopenia, however with normal CD4/CD8 ratio. Finally CNS biopsy revealed T-cells close to blood vessels – a pattern typical for cerebral GvHD. Immunosuppressive treatment was started with high dose steroids in combination with mycophenolatmofetil (MMF). She recovered rapidly and became completely awake within one week. After 9 months of immunosuppression the patient is competent in activities of daily living. Conclusions. GVHD of the central nervous system (CNS) is a rare disease after allogeneic stem cell transplantation. The absence of lymphocytes in the cerebrospinal fluid and normal CD4/CD8 ratio in peripheral blood does not exclude GvHD of the CNS. CNS biopsy should be considered to confirm the diagnosis. This case demonstrates that CNS involvement can be the only manifestation of chronic GvHD. Immunosuppressive therapy may provide an impressive benefit in these patients. 132 | Der Pathologe · Supplement 1 · 2012 FR-P-154 Tuberculous lymphadenitis and mycobacterium-negative granulomatous disease in patients receiving Imatinib mesylate (Glivec) treatment for gastrointestinal stromal tumors (GIST) A . Agaimy1 , A . Stegmann2 , A . Mackensen3 , N . Meidenbauer3 1Friedrich-Alexander University of Erlangen, Institute of Pathology, Erlangen, 2Friedrich-Alexander University of Erlangen, Department of otorhinolaryngology, head and neck surgery, Erlangen, 3Friedrich-Alexander University of Erlangen, Medical Clinic 5 , Erlangen Aims. Imatinib mesylate (IM) represents the standard treatment for patients with BCR-ABL-positive chronic myelogenous leukemia (CML) and is the first line adjuvant and palliative treatment modality for those with disseminated or inoperable gastrointestinal stromal tumor (GIST). IM is not known to be associated with an elevated risk for tuberculosis, since only five patients have been reported to date who developed tuberculosis during or after IM treatment for cmL (n=4) and GIST (n=1). Methods. We describe our experience with 3 patients (45–79 yrs of age) who developed necrotizing (caseating) granulomatous disease during IM treatment for GIST. Mean duration of treatment with Imatinib was 9.5 (range: 9–48) months. Results. In 3 patients, enlarged lymph nodes with increased metabolism in Fludoxyglucose-Positron emission tomography (FDG-PET)-computer tomography-examinations were detected and resected. Affected sites were subcarinal/mediastinal (1), mediastinal/supraclavicular (1) and periparotideal cervical (1) lymph nodes. Histologic examination revealed necrotizing granulomatous disease suggestive of infection with M. tuberculosis or sarcoidosis. Sputum examination was negative for acid fast bacilli in all patients and DNA was negative for M. tuberculosis and other mycobacteria in two cases. However, M. tuberculosis was detected by PCR in the lymph node of one patient who was then successfully treated by antituberculous agents. All other patients received no anti-tuberculous therapy and were on complete response or stable neoplastic disease without evidence of progressive lymphadenopathy or lung lesions suggestive of active tuberculosis. Leucocyte and lymphocyte counts have been within normal limits throughout treatment with IM. Conclusions. Our observations underline the necessity to sample enlarged or metabolic active lymph nodes developing during IM treatment for timely diagnosis and appropriate treatment of these rare complications. Follow up without treatment is safe for lesions without detection of M. tuberculosis by PCR. More studies are needed to clarify the potential causal relationship to IM treatment and to sufficiently explore the pathogenesis of lesions that were negative for M. tuberculosis. FR-P-155 Therapeutic Polo-like kinase 1 inhibition results in mitotic arrest and subsequent cell death of leukemic cells in acute myeloid leukemia C . Münch1 , A .M . May1 , A .V . Pfister1 , K . Thurig1 , M . Lübbert2 , R . Wäsch2 , T . Taube3 , S . Lassmann1 , M . Werner1 1 2 University Freiburg Medical Center, Institute of Pathology, Freiburg, University Freiburg Medical Center, Department of Hematology and Oncology, Freiburg, 3Boehringer Ingelheim Pharma GmbH & Co . KG, Clinical Research Aims. The mitotic kinase Polo-like kinase 1 (Plk1) is an important cell cycle regulator which is frequently overexpressed in acute myeloid leukaemia (AML). Our previous examination of AML blasts in pre- and posttreatment bone marrow biopsies of AML patients treated with the small molecule PLK1 inhibitor BI2536 revealed an increase of aberrant mitotic figures and apoptotic cells 24 hours after administration. The aim of this study was to extend this examination by investigating the effects of therapeutic PLK1 inhibition on AML cell lines and lymphoblastoid cell lines (LCLs) from healthy controls. Methods. Five AML cell lines (HL-60, KG1, OCIM2, NB4 and THP1) and two LCLs (LCL1 and LCL2) were cultured for 24 and 48 hours with
Page 2 and 3:
Inhalt Der Pathologe · Supplement
Page 4 and 5:
Inhalt Der Pathologe · Supplement
Page 6 and 7:
Editorial Liebe Kolleginnen und Kol
Page 8 and 9:
Kolorektales Karzinom 2 VO-005 Tran
Page 10 and 11:
Keynote Lecture VO-014 Genetic dete
Page 12 and 13:
(AMACR, FASN, GOLM1, GSP-pi, ERG) t
Page 14 and 15:
to assess the correct rate of R1 re
Page 16 and 17:
DNA damage, and cytotoxic drugs. Au
Page 18 and 19:
HCV-positive formalin-fixed and par
Page 20 and 21:
well as MET activation were examine
Page 22 and 23:
or BRAF mutation, c-MYC and SIRT1 e
Page 24 and 25:
AG Pneumopathologie III DO-032 Remo
Page 26 and 27:
immature granulopoiesis showed a st
Page 28 and 29:
ned, if well-defined mantle zones,
Page 30 and 31:
phomas). Most prominent gains or am
Page 32 and 33:
DO-062 Tumor-associated macrophages
Page 34 and 35:
DO-080 DOG1: an immunohistochemical
Page 36 and 37:
AG Oralpathologie DO-087 Detection
Page 38 and 39:
DO-094 Do activated fibroblasts inf
Page 40 and 41:
DO-101 Histopathological analysis o
Page 42 and 43:
DO-108 Identification of potential
Page 44 and 45:
Conclusions. In conclusion, 454 par
Page 46 and 47:
subgroup of patients with B-Raf mut
Page 48 and 49:
DO-002b Impact of terminologies in
Page 50 and 51:
Methods. We performed MCPyV-FISH of
Page 52 and 53:
FR-013 HPV-genotype distribution in
Page 54 and 55:
Methods. Three different techniques
Page 56 and 57:
(i.e. investment in equipment and e
Page 58 and 59:
FR-032 COLD-PCR: a powerful tool in
Page 60 and 61:
dissection (MBLND) technique to imp
Page 62 and 63:
SO-005 Prevalence of mutations in s
Page 64 and 65:
SO-011 Methylation profiling and in
Page 66 and 67:
more effective than SAHA alone and
Page 68 and 69:
SO-022 Specialized pathology review
Page 70 and 71:
SO-027 Ki-67 in mitotic score group
Page 72 and 73:
nificantly associated with advanced
Page 74 and 75:
liver did not correlate with the mu
Page 76 and 77:
AG Paidopathologie II SO-046 Overex
Page 78 and 79:
Results. Histomorphology of the ova
Page 80 and 81:
p42 and p27 have not yet been inves
Page 82 and 83: SO-068 Recent advances in understan
Page 84 and 85: SO-075 A novel, dual role of CCN3 i
Page 86 and 87: Pancreatic Adenocarcinoma SG-137 Se
Page 88 and 89: sease and 30 colon cancer serum sam
Page 90 and 91: Conclusions. Although CRC is charac
Page 92 and 93: differentiated and TNM stage III or
Page 94 and 95: pressed moderate levels of the prot
Page 96 and 97: FR-P-037 MALDI imaging reveals COX7
Page 98 and 99: in the context of therapy response
Page 100 and 101: on primary cells of wild-type and c
Page 102 and 103: chemotherapy was recommended and co
Page 104 and 105: aims are twofold: 1) to verify the
Page 106 and 107: Results. Her2/neu status in TMAs an
Page 108 and 109: prognostic impact was found in rect
Page 110 and 111: provided information on survival ti
Page 112 and 113: Methods. Biopsy specimens from 430
Page 114 and 115: the OS rate was 53% for patients wi
Page 116 and 117: FR-P-098 Immunohistological stainin
Page 118 and 119: FR-P-104 Histopathological evaluati
Page 120 and 121: within the earliest morphologic det
Page 122 and 123: Conclusions. In primary imaging a g
Page 124 and 125: fibrotic neointma development as we
Page 126 and 127: FR-P-129 Alpha-1-antitrypsin-PiZ-an
Page 128 and 129: FR-P-136 The expression of central
Page 130 and 131: cosa and in the periarterial tissue
Page 134 and 135: or without different concentrations
Page 136 and 137: Results. In 18 cases (79%) the caus
Page 138 and 139: FR-P-168 Autopsy findings in a 2-ye
Page 140 and 141: FR-P-174 MPGN-like glomerulonephrit
Page 142 and 143: Poster: Gynäkopathologie und Mamma
Page 144 and 145: SA-P-011 Genetic aberrations of pre
Page 146 and 147: Mechanismen, die eine Progression d
Page 148 and 149: internet server. A network of inter
Page 150 and 151: Methods. HE-gefärbte Schnittpräpa
Page 152 and 153: Conclusions. The newly released 450
Page 154 and 155: and desmoplastic reaction are diffe
Page 156 and 157: one patient revealed more than 9 mi
Page 158 and 159: situation, the V600E mutation in th
Page 160 and 161: SA-P-064 Evolution of molecular pat
Page 162 and 163: nic markers such as myf4 and MyoD1
Page 164 and 165: Conclusions. To our knowledge, this
Page 166 and 167: SA-P-085 Expression of the eukaryot
Page 168 and 169: Results. The papillary RCC type II
Page 170 and 171: SA-P-098 Male infertility: assessme
Page 172 and 173: SA-P-104 Process oriented scientifi
Page 174 and 175: Conclusions. The IBDW offers a uniq
Page 176 and 177: L Lasitschka F. DO-046 Lehmann A. F
show all

96. Jahrestagung der Deutschen Gesellschaft für Pathologie e. V ...

Create successful ePaper yourself

Delete template?

Save as template?