96. Jahrestagung der Deutschen Gesellschaft für Pathologie e. V ...

Abstracts
is known about the functionality of SFRP1. In this project we have analyzed
the functional consequences of a SFRP1 re-expression in human breast
cancer cell lines. We also conducted a systematic expression analysis
to determine possible links to other pathways after SFRP1 re-expression.
Methods. Using standardized methods SFRP1 overexpressing clones of
two human breast cancer cell lines, BT20 (basal-like) and SKBR3 (luminal-like),
were generated and analyzed in cell-culture based assays. The
ability of SFRP1 expressing BT20 clones to grow in nude mice will be
also tested. Using DNA array expression profiling, we searched for genes
activated or repressed after forced re-expression of SFRP1 in these two
tumour cell lines (“SFRP1 target genes”). Validation of differential gene
expression was performed by real-time PCR comparing stable SFRP1
and control clones.
Results. SFRP1 re-expression in stable clones of two breast cancer cell
lines (BT20 and SKBR3) was validated on mRNA and protein level. We
found a correlation between SFRP1 expression and the growth behaviour
of both breast cancer cell lines in functional assays. SFRP1 re-expression
resulted in a significant reduced proliferation (p