The Toxicologist - Society of Toxicology
The Toxicologist - Society of Toxicology
The Toxicologist - Society of Toxicology
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1371 PON1 Q192R POLYMORPHISM ANALYSIS IN<br />
SOUTHERN CARDIAC PATIENTS.<br />
M. Dail, A. Crow, H. Coombes, E. Meek, H. Chambers and J. Chambers.<br />
Center for Environmental Health Sciences, Mississippi State University, MS State, MS.<br />
Paraoxonase (PON1) hydrolyzes paraoxon, the active metabolite <strong>of</strong> the insecticide<br />
parathion. <strong>The</strong> human PON1 gene has several single nucleotide polymorphisms<br />
(SNPs). <strong>The</strong> SNP involving a glutamine (Q) to arginine (R) substitution at codon<br />
192 may be associated with the ability <strong>of</strong> PON1 to hydrolyze oxidized lipids, which<br />
protects against atherosclerosis. <strong>The</strong> question <strong>of</strong> whether the Q192R polymorphism<br />
is associated with cardiac disease is still open. Since southerners have the<br />
country’s highest rate <strong>of</strong> cardiac disease, we collected blood and demographic data<br />
from 197 patients in a Mississippi cardiovascular clinic to compare the functional<br />
genotypes derived from serum PON1 hydrolytic activity to parameters <strong>of</strong> cardiac<br />
health. <strong>The</strong> multivariable model generated suggested that functional genotypic differences<br />
were insignificant compared to classic factors <strong>of</strong> atherosclerotic risk, but<br />
many <strong>of</strong> the patients were difficult to sort. To increase clarity, we sorted patients by<br />
their actual genotype and reexamined associations. Genomic DNA was isolated<br />
from blood and the 192 codon area was amplified by PCR. Since there is an AlwI<br />
restriction site at codon 192, the QQ, RR, and QR SNPs yield different digest patterns.<br />
<strong>The</strong> prior work found that Caucasians most <strong>of</strong>ten display the QQ functional<br />
genotype while African Americans have the RR functional genotype. This was confirmed.<br />
Other significant relationships: QQs are more <strong>of</strong>ten smokers whereas RRs<br />
are nonsmokers. QQs and RRs are more likely to have a family history <strong>of</strong> cardiac<br />
disease than QRs. HDL levels <strong>of</strong> RR patients were found to be significantly higher<br />
(P = 0.012) than those having a QQ genotype. RR patients were significantly<br />
younger (P = 0.02) than either QR or QQ patients. No significant relationships<br />
were seen between genotype and personal history <strong>of</strong> cardiac disease, statin use, or<br />
diabetes. No significant differences were seen among the three genotypes with regard<br />
to triglycerides, height, weight, waist size, LDL, or total cholesterol (Support<br />
NIHES015170)<br />
1372 ANALYSIS OF POLYMORPHISMS OF ASTHMATIC<br />
CHILDREN FROM COAHUILA, MEXICO EXPOSED TO<br />
ENVIRONMENTAL TOBACCO SMOKE (ETS).<br />
B. Muñoz-Soto 1 , B. S. Barrón-Vivanco 1 , A. López-Moya 1 , I. Romero-Toledo 1 ,<br />
C. López-Campos 2 , J. J. Magaña-Aguirre 3 and A. Albores 1 . 1 <strong>Toxicology</strong>, Cinvestav-<br />
IPN, México, Mexico, 2 Pediatrics, UMAE-IMSS 71, Torreon, Coahuila, Mexico and<br />
3 Human Genetics, INR, Mexico City, Mexico.<br />
Recent studies argue that asthma prevalence in Mexico has increased 15% in the<br />
last 10 years; the constant exposure to allergens and environmental pollutants such<br />
as tobacco smoke is considered as one <strong>of</strong> the principal etiological factors. <strong>The</strong> vast<br />
majority <strong>of</strong> asthmatics are children; therefore, the aim <strong>of</strong> this study was to investigate<br />
the association between ETS exposure and some genetic polymorphism in<br />
asthmatic children. Our pilot study includes a population from Torreon, Coahuila,<br />
located in Northeast Mexico. Study groups included asthmatic children diagnosed<br />
according to ISAAC parameters (n=114) and a control group (n=100). <strong>The</strong>se<br />
groups were subdivided in ETS-exposed children and non-exposed children, according<br />
to the parent’s smoking habits. Spirometric studies and urine cotinine<br />
analysis were also performed. Real time PCR assays were used for genotyping IL13<br />
(rs20541 and rs1800925), GSTP1 (rs1695), TNF (rs18000629) TSLP<br />
(rs1837253), ADBR2 (rs1042713) polymorphisms whereas conventional PCR was<br />
used for genotyping CYP1A1 (rs1048943). Our findings indicate that smoker’s<br />
children showed higher cotinine levels than controls. On the other hand, we found<br />
no association between variants <strong>of</strong> IL13, GSTP1, TNF or CYP1A1 with asthma<br />
and exposure to ETS, however, other studies have shown this association.<br />
Remarkably, girls seems to be more susceptible to develop asthma (p=0.057); a<br />
strong association with TSLP (rs1837253) (p=0.0271) and marginal associations <strong>of</strong><br />
ADBR2 (rs1042713) (p=0.099) with asthma was also observed. In addition, the<br />
TSLP mutated allele in asthmatic girls was also associated (p=0.072). This is the<br />
first study <strong>of</strong> asthma-related polymorphisms in Mexican children exposed to ETS.<br />
294 SOT 2011 ANNUAL MEETING<br />
1373 CYP1A1*2C, EPHX1T113H, EPHX1H139R, AND NQO1*2<br />
POLYMORPHISMS ARE INVOLVED IN THE GENETIC<br />
DAMAGE CAUSED IN CHILDREN EXPOSED TO PAHS<br />
AND BENZENE.<br />
M. Sánchez-Guerra 1 , N. A. Pelallo-Martínez 2 , F. Díaz-Barriga 2 , L. Carrizales-<br />
Yáñez 2 , L. Hernández-Cadena 3 , M. E. Gonsebatt 4 and B. Quintanilla-Vega 1 .<br />
1 <strong>Toxicology</strong> Department, Cinvestav-IPN, Mexico City, Mexico, 2 Faculty <strong>of</strong> Medicine,<br />
UASLP, San Luis Potosí, Mexico, 3 National Institute <strong>of</strong> Public Health, Cuernavaca,<br />
Morelos, Mexico and 4 IIB, UNAM, Mexico City, Mexico.<br />
Polycyclic aromatic hydrocarbons (PAHs) and benzene are pollutants <strong>of</strong> urban<br />
cities and are considered genotoxic. Both compounds are metabolized and some enzymes<br />
such as CYP1A1 and CYP1B1 (activation) and NQO1 and GSTM (deactivation)<br />
and EPHX1 with both activities are polymorphic. We performed a crosssectional<br />
study in children (6 to 10 years old, n=77) from primary schools located<br />
in the vicinities <strong>of</strong> the main industrial complex <strong>of</strong> Coatzacoalcos County, Veracruz,<br />
Mexico, an industrialized area with 80% <strong>of</strong> country’s petrochemical activity, to<br />
evaluate the genetic damage <strong>of</strong> PAHs and benzene exploring the role <strong>of</strong> some metabolizing<br />
enzyme polymorphisms. Urinary 1-OHP levels and trans, trans muconic<br />
acid (t,t MA) as biomarkers <strong>of</strong> PAHs and benzene exposure, respectively, were determined<br />
by HPLC and CYP1A1*2C, CYP1B1*3, EPHX1T113H, EPHX1H139R,<br />
NQO1*2 and GSTM1*0 genetic polymorphisms by real time or conventional<br />
PCR. DNA damage was evaluated in lymphocytes by the Comet assay (tail moment).<br />
Thirteen percent <strong>of</strong> children had 1-OHP levels higher than 1.4 μmol/mol<br />
creatinine (NOAEL) and 32% had higher t,t-MA levels than the biological exposure<br />
index in the workplace (500 μg/g creatinine). DNA damage was significantly<br />
associated only with 1-OHP levels: children with 1-OHP levels above 1.4<br />
μmol/mol creatinine showed a 23% increase <strong>of</strong> DNA damage, adjusted by t,t-MA<br />
(p=0.027). On the other hand, the DNA damage was higher in CYP1A1*2C homozygous<br />
children with 1-OHP levels above the NOAEL (54%, p=0.032); while<br />
children carrying one allele <strong>of</strong> NQO1*2 or <strong>of</strong> EPHX1H139R polymorphisms had<br />
lower DNA damage (-37%, p=0.039 and -49%, p=0.007, respectively). <strong>The</strong>se results<br />
suggest that polymorphisms <strong>of</strong> the studied metabolizing enzymes may have an<br />
important role in the development <strong>of</strong> adverse effects <strong>of</strong> urban environmental exposed<br />
children.<br />
1374 USING OF PCR-RFLP OF CYTOCHROME B GENE FOR<br />
HAIR IDENTIFICATION IN HUMAN AND SOME NON-<br />
DOMESTIC ANIMALS.<br />
M. H. Ghoneim, A. A. Abou-Hadeed, A. M. Alkelch and M. R. Awad-allah.<br />
<strong>Toxicology</strong>&Forensic Medicine, Zagazig University, Zagazig, Egypt.<br />
PCR and PCR-RFLP methods were used to differentiate between human and some<br />
non domestic animals. DNA from hair was extracted to amplify a mitochondrial<br />
cytochrome b (cyt b) gene segment (358 bp) using universal primer followed by<br />
agarose gel electrophoresis. <strong>The</strong> total cyt b segment was digested with 4 restriction<br />
endonucleases,( Alu I, HaeIII, HinfI and Taq I ) and the resulting fragments were<br />
resolved through electrophoresis. <strong>The</strong> different specific electrophoretic patterns and<br />
total restriction fragments were observed in each <strong>of</strong> the studied species. <strong>The</strong> restriction<br />
enzyme Alu I yielded 2 fragments <strong>of</strong> different sizes in all animal species. while<br />
no cleavage was observed in human hair. <strong>The</strong> digestion <strong>of</strong> PCR products with<br />
HaeIII restriction enzyme revealed restriction fragments patterns <strong>of</strong> different sizes<br />
in human and all species except in Bear. Using <strong>of</strong> HinfI restriction enzyme produced<br />
2 or 3 fragments <strong>of</strong> different sizes for human and all species except Lama.<br />
When the amplified cyt b segment was digested by Taq I restriction enzyme it gave<br />
2 bands <strong>of</strong> different sizes except Barbary sheep. <strong>The</strong>re was variation in size <strong>of</strong> segments<br />
in human and different species.<br />
1375 STEM CELL REPONSES OF MURINE HIPPOCAMPUS<br />
FOLLOWING TRIMETHYLTIN INTOXICATION.<br />
B. C. Weig, K. R. Reuhl and H. E. Lowndes. Pharmacology & <strong>Toxicology</strong>, Rutgers<br />
University, Piscataway, NJ.<br />
Stem cells from the developing mammalian brain persist in adults in the subventricular<br />
zone (SVZ) and the hippocampal subgranular zone (SGZ). <strong>The</strong> response <strong>of</strong><br />
SVZ and SGZ stem cells to hippocampal injury was studied in two-month old<br />
male C3H mice treated with trimethyltin (TMT). <strong>The</strong> fluorescent marker spDiI<br />
was injected unilaterally into the lateral ventricle <strong>of</strong> mice to label migrating SVZ<br />
cells. Bromodeoxyuridine (BrdU) was administered (80 mg/kg; ip) to label proliferating<br />
progenitor cells prior to treatment with TMT (2.7 mg/kg; ip). Sagittal brain<br />
sections taken 7 or 28d later revealed spDiI+ cells in the hippocampus <strong>of</strong> control<br />
mice at 7d, with a trend toward an increased number cells at 28d (p=0.0523). TMT<br />
injury had no effect on the number <strong>of</strong> BrdU+ or spDiI+ cells in the hippocampus at