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4A<br />

Investigation<br />

The Human Karyotype<br />

The genetic material in human cells is contained in<br />

46 chromosomes, arranged in 23 pairs. A karyotype<br />

is used to organize and analyze the genetic material<br />

of individuals.<br />

Question<br />

What information can you obtain from a human<br />

karyotype?<br />

Experimental Design<br />

In this investigation, you will use an image of human<br />

chromosomes to construct a karyotype.<br />

Materials<br />

• image of human chromosomes<br />

• scissors<br />

• glue<br />

• paper<br />

• marker<br />

Procedure<br />

1. Cut out all the individual chromosomes.<br />

2. Arrange the chromosomes in homologous<br />

pairs. What features can you use to arrange<br />

them into pairs?<br />

3. Once you have matched the chromosomes, arrange<br />

them in ordered pairs, as shown in Figure 1.<br />

INQUIRY SKILLS<br />

Questioning<br />

Hypothesizing<br />

Predicting<br />

Planning<br />

Conducting<br />

Recording<br />

Analyzing<br />

Evaluating<br />

Synthesizing<br />

Communicating<br />

4. Paste your chromosome pairs, in order, on a<br />

blank sheet of paper.<br />

5. Number your chromosome pairs from 1 to 23.<br />

6. Compare your karyotype with your classmates’<br />

karyotypes. Indicate the gender of each of your<br />

classmates’ karyotypes.<br />

Analysis<br />

(a) How many pairs of chromosomes have you<br />

matched?<br />

(b) Did all the chromosomes have a matching<br />

partner? If not, explain why.<br />

(c) Is your karyotype male or female? Explain how<br />

you know.<br />

Evaluation<br />

(d) What features of the chromosomes did you use<br />

to match them?<br />

(e) Are there other features you could have used?<br />

(f) Were there any chromosomes that were difficult<br />

to match?<br />

Synthesis<br />

(g) What type of information can be gained from<br />

a karyotype?<br />

(h) Would karyotypes from the same family look<br />

the same? Explain.<br />

(i) Why would it be useful to compare karyotypes<br />

of family members?<br />

2000X<br />

Figure 1 A human karyotype. The homologous pairs are numbered.<br />

NEL<br />

Chapter 4 Investigation 139

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