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Biology - HOT Science Lab

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Teacher<br />

Investigating Bacterial Growth<br />

NGSSS:<br />

SC.912.L.14.52 Explain the basic functions of the human immune system, including specific and<br />

nonspecific immune response, vaccines, and antibiotics. (AA)<br />

Purpose of <strong>Lab</strong>/Activity: The purpose of this activity is to observe how bacteria grow in a<br />

culture medium through time, and observe if antiseptics, antibiotics, and disinfectants will inhibit<br />

bacterial growth. The lab will take place over 2 days. After preparing for day 1, you will need to<br />

prepare for the second day, 5 days later.<br />

Prerequisites:<br />

The students will know the differences between prokaryotic cells and eukaryotic cells<br />

The students will understand how under certain conditions populations could grow<br />

exponentially.<br />

The students will have prior knowledge of characteristic structures and functions that<br />

make cells distinctive.<br />

The students will know processes in a cell classified broadly as growth, maintenance,<br />

reproduction, and homeostasis.<br />

Materials (per group):<br />

1 agar plate<br />

Sample of bacteria<br />

Inoculating loop or cotton swab<br />

Forceps<br />

Tape<br />

1 wax pencil/Sharpie<br />

1 metric ruler<br />

Distilled water<br />

Blank paper disk<br />

3 disks immersed in either antibiotic,<br />

antiseptic, or disinfectant solutions<br />

1 paper disk not immersed with any<br />

fluid<br />

Penicillin or similar antibiotic<br />

Bleach<br />

Mouthwash<br />

Procedures: Day of Activity:<br />

Before<br />

activity:<br />

During<br />

activity:<br />

What the teacher will do:<br />

a. Prepare 1 clean Petri dish with agar for each group along with the<br />

remaining materials needed for the lab.<br />

b. Introduce the activity by asking the following questions:<br />

1. What effect will a microbe inhibitor have on the growth of bacteria<br />

colonies<br />

2. Are there differences in the efficacy of the different microbial inhibitors<br />

used in the lab<br />

3. What are some ways that you can measure the differences of the<br />

efficacy of each solution<br />

What the teacher will do:<br />

a. Engage student thinking during activity by asking the following questions:<br />

1. What is the role of agar<br />

2. Why do you need to label the Petri dish so carefully<br />

3. Why do you need to keep the Petri dish covered<br />

4. Why do you need to control the temperature of the bacteria culture so<br />

<strong>Biology</strong> HSL Page 287<br />

Curriculum and Instruction

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