AvaRaman Operating manual AvaSoft Raman 7.3 November 2008

The PTFE spectrum will be used to calculate and display the laserwavelength after each scan. By
clicking the “Save Calibration” button, this wavelength will be stored.
2.3.2.6.8 Setup Menu: Options – Use Non-Linearity Correction
This option will be available only if the Raman System has been calibrated to correct for nonlinearity
behaviour of the detector, and the resulting calibration file with the name .nl
has been installed in the folder from where AvaSoft-Raman is started. stands for the
serialnumber of the spectrometer, e.g. 0405009S1.nl.
Correcting for non-linearity can be useful if you are not only interested in the location of the peaks
in a raman spectrum, but also in the (relative) intensity, compared to other peaks in the spectrum.
To be able to compare the intensity between peaks, the software also needs to correct for detector
sensitivity, grating sensitivity etc.. This can be done by loading an irradiance calibration, as
described in section 3.3.1: View Normalized Counts.
2.3.2.6.9 Setup Menu: Options – Use Baseline Correction
Fluorescence signal often interferes with the
Raman signal of interest. In AvaSoft, the
fluorescense signal can be subtracted from
the measured spectrum by applying the
interactive baseline correction function.
Baseline correction can be applied on spectra
that were saved before, but also on spectra
that are measured online.
By clicking the “Use Baseline Correction”
menu option, the window at the right is
displayed. The dropdown box (default
“Master” ) shows all active spectra in the
main window, including the displayed spectra
(File – Display Saved Graph). Select the
spectrum for which the baseline correction needs to be applied in the dropdown box. In the
example below we selected an earlier saved graph called NAFTA2005.ROH.
40 AvaRAMAN manual.doc Nov-08
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