Inaugural ASAS–CAAV Asia Pacif ic Rim Conference Abstracts

25 Genetic evaluations for measures of the milk flow curve in theItalian Brown Swiss. K. A. Gray* 1 , F. Vacirca 2 , A. Bagnato 2 , A. Rossoni 3 , A.B. Samoré 2 , J. P. Cassady 1 , and C. Maltecca 1 , 1 North Carolina State University,Raleigh, NC, USA, 2 Università degli studi di Milano, Milano, Italy, 3 ItalianBrown Swiss Breeders Association, Bussolengo, Italy.The objective was to estimate (co)variance components among milk release,somatic cell score, milk yield, and udder traits. Single milking flow records fromcows (n = 37,511) representing 1,592 herds were used. Three statistical modelswere used: Model 1 included test-day data [milk yield (TD-MY), somatic cellscore (TD-SCS), maximum milk flow (MMF), average flow (AVGF), plateautime (TP), descending time (DT), and total milking time (TMT)]; Model2 included milk release parameters adjusted to the sixth month in lactation(A-TMT, A-MMF, A-AVGF, A-TP and A-DT) as well as total udder score(TUS), udder depth (UD), 305-d milk yield (305-MY), and somatic cell score(305-SCS) as the dependent variables; Model 3 included A-TMT, 305-MY,305-SCS, and ratios of MMF over TMT (R1), TP (R2), and DT (R3) to estimatethe relationship between the shape of the milk release curves and milking traits.Models 1 and 2 resulted in similar heritabilities for milkability traits rangingfrom 0.06 to 0.43 with genetic correlations between production traits and flowtraits ranging from low to moderate values. Positive genetic correlations werefound among production, SCS, and milkability traits. In Model 3, R1 had thehighest heritability of the ratio traits (0.35) with high genetic correlations withR2 and R3 and a low correlation with 305-SCS and no correlation with 305-MY.Estimated responses to selection over 5 generations were also calculated usingA-AVGF and 2 different indexes, which included either flow or ratio traits. Inconclusion, data collected from portable flowmeters may be used to select forimproved milkability traits. Complete data to describe overall release of milkmay be used to reduce management costs while maintaining milk production.Key Words: milk release, Brown Swiss, genetic parameters26 Effects of dietary energy density on plasma glucose andlipid profile, morphofunctional aspects, and chemical characteristics inadipose tissue of finishing pigs. Y. Liu* 1 , J. Chao 1 , Y. Yin 1,2 , Y. Hou 1 , H. Zhu 1 ,and X. Kong 2 , 1 Hubei Key Laboratory of Animal Nutrition and Feed Science,Wuhan Polytechnic University, China, 2 Institute of Subtropical Agriculture, theChinese Academy of Sciences, China.This study was conducted to investigate the effects of dietary energy densityon plasma glucose and lipid profile, morphofunctional aspects, and chemicalcharacteristics in adipose tissue of finishing pigs. Thirty finishing pigs (initialBW 41.3 ± 4.0 kg) were given free access to low-energy density [LD, 12.82MJ of digestible energy (DE)/kg] diet, medium-energy density (MD, 14.24MJ of DE/kg) diet, or high-energy density (HD, 15.66 MJ of DE/kg) diet. Thediets had equal concentrations of crude protein. On d 53, blood samples werecollected, and then pigs were killed to collect the dorsal subcutaneous (ST),abdominal (AT), and mesenteric (MT) adipose tissues. The LD pigs had lowerplasma glucose and higher triglyceride (TG) than MD and HD pigs, and higherratio of low density lipoprotein cholesterol/high density lipoprotein cholesterol(LDL/HDL) than HD pigs (P < 0.05). Compared with the MD group, malatedehydrogenase activity was significantly increased in ST and MT of HD group(P < 0.05) and was significantly decreased in MT of LD group (P < 0.05).Compared with MD pigs, glucose-6-phosphate dehydrogenase activity wasincreased in all three depots of HD pigs (P < 0.05). Both HD and LD dietsincreased the adipocyte size (adipocyte diameter, area, and volume) in AT andMT (P < 0.05). Pigs fed HD diet had greater cell proliferation index in STcompared to the other two feeding groups (P < 0.05). Compared with MD pigs,a decreased apoptosis index was seen in ST of HD pigs, and in AT of LD pigs (P< 0.05), and in MT of HD pigs and LD pigs (P < 0.05). Peroxisome proliferatoractivatedreceptor γ (PPARγ)-positive percentage was elevated in ST and MTof HD pigs compared with MD pigs and LD pigs (P < 0.05) but decreasedin ST of LD pigs compared with MD pigs (P < 0.05). These results suggestthat dietary energy density could regulate fat deposition in finishing pigs. Itis possible that high energy diets may induce fat deposition via up-regulatingPPARγ expression.Key Words: energy, finishing pig, adipose tissueNonruminant Nutrition I27 Effect of dietary supplementation of fish oil for lactating sowsand weaned piglets on piglet T helper cell polarization. J. Luo 1 , F. Huang 1 ,C. Xiao 1 , W. Chen 1 , S. Jiang 2 , and J. Peng* 1 , 1 Department of Animal Nutritionand Feed Science, College of Animal Science and Technology, HuazhongAgricultural University, Wuhan, Hubei, P. R. China, 2 Key Laboratory of SwineBreeding and Genetics of Agricultural Ministry, College of Animal Science andTechnology, Huazhong Agricultural University, Wuhan, Hubei, P. R. China.The present study was designed to investigate the effect of dietary fish oilsupplementation on piglet T helper cell (Th) polarization in relation to its impacton piglet serum interferon γ (IFN-γ) and interleukin 10 (IL-10) concentrationsand splenic expression of Th1/Th2 characteristic genes. The diets of 18gestating sows containing 7% lard (LD; n = 10) or 7% fish oil (FO; n = 8) werefed from 10 d before parturition to weaning on d 28. At weaning, a split plotexperiment was designed; 56 piglets, 28 (half female and half castrated male)each from sows fed FO diet or LD diet, were subdivided into 4 groups of 14piglets (one female and one castrated male per pen) based on both sow dietduring lactation and postweaning piglet diet (L had 7% LD and F had 7% FO):LL, LF, FL, FF and were fed the 7% FO or LD diet from d 35 to 70. Serumconcentrations of IFN-γ and IL-10 were measured by ELISA, and Th1/Th2related genes mRNA abundances in spleen were analyzed by relative reversetranscription-PCR. The results showed that piglets fed with fish oil diet duringpostweaning tended to have higher serum IFN-γ/IL-10 ratio (P = 0.09) thanlard diet-fed piglets. Late gestation-lactating fish oil feeding increased splenicIL-12b, IL-12 receptor β2 (IL-12Rβ2), IL-2, and IFN-γ gene expression (P