Inaugural ASAS–CAAV Asia Pacif ic Rim Conference Abstracts

0.05). However, diets with 100 or 200 mg/kg added XOS reduced (P < 0.05) thediarrhea rate compared to the control. Pigs fed a diet with 200 mg/kg XOS hadthe lowest diarrhea rate compared with other treatments (P < 0.05). However,pigs supplemented with XOS tended to have lower F:G and greater ADFI. InExperiment 2, a total of twenty 55-d-old pigs were randomly assigned to one of5 dietary treatments with 4 replicates of 1 piglet each. Dietary treatments werethe same as Experiment 1. The experiment consisted of a 7-d pre-adaptationand a 7-d collection of fecal samples. Crude protein, GE, NDF, and DM of feedand fecal samples were determined. Supplementation of XOS and aureomycinincreased (P < 0.05) the apparent digestibility of CP and NDF in contrast to thecontrol. The apparent digestibility of DM and GE were increased (P < 0.05) byaddition of 100 mg/kg XOS, but were reduced (P < 0.05) by supplementationof 100 mg/kg aureomycin, compared with other treatments. In conclusion,supplementation of XOS can improve energy and nutrient utilization of piglets,reduce the incidence of diarrhea, and tended to increase growth performance.Key Words: xylo-oligosaccharides, pig, apparent digestibilityM152 The study on lysine requirement of neonatal piglets.Zongyong Jiang*, Kun Huang, Chuntian Zheng, Yingcai Lin, and ShouqunJiang, The Key Laboratory of Animal Nutrition and Feed Science (South China)of Ministry of Agriculture, Guangdong Public Laboratory of Animal Breedingand Nutrition,Institute of Animal Science,Guangdong Academy of AgriculturalSciences, Guangzhou, Guangdong, P.R.China.This experiment investigated the effects of dietary lysine levels on the growthperformance, nitrogen utilization and hormone levels in plasma in the neonatalpiglets, and determined dietary lysine requirements of the neonatal piglets.According to single-factor test design, forty 4-d-old neonatal piglets (LargeWhite × (Yorkshire × Duroc), 1.90 ± 0.05 kg) were selected from litters of15 sows and randomly assigned to 5 dietary treatments according to litterand body weight, each treatment with 4 replicates, and the other six pigletswere slaughtered to determine the body composition. The dietary lysine levelswere designed as: 1.27% (the control), 1.37%, 1.47%, 1.57% and 1.67%,respectively. The results showed that with the increase of dietary lysine level,the weight of piglets increased continuously, the 1.47% and 1.67% lysinegroups were significantly higher than the control group (P < 0.05). There wereno significant differences in ADFI, ADG and feed:gain ratio (F/G) of 4- to14-d-old piglets among five groups (P > 0.05). From 15 to 21 d and from 4to 21 d, ADFI of the 1.57% and 1.67% groups were significantly higher thanthat of the control group (P < 0.05), and ADG of the groups from 1.47% to1.67% lysine levels were significantly higher than that of the control group (P< 0.05). F/G decreased significantly (P < 0.05), of which the treatment groupswere significantly lower than that of the control group (P < 0.05). BVN from1.47% group to 1.67% group was higher significantly than that of 1.27% groupand 1.37% group. Dietary Lysine levels had no influence on energy and fatdeposition rate in piglets (P > 0.05). With the increase of dietary lysine level,protein deposition rate ascended, and deposition rate in 1.47%–1.67% groupswere higher than that of 1.27%–1.37% group (P < 0.05), where the highestappeared in 1.67% group. From these results, it can be concluded that optimaldietary lysine level for neonatal piglet (4 to 21-d-old) is 1.57%, that is, 73.1 gLys/kg CP or 0.85 g Lys/MJ DE, or 2.43 g Lys/d.Key Words: lysine requirement, growth performance, neonatal pigletsM153 Effect of hydrolyzed wheat gluten on growthperformance, cell immunity and serum biochemical indexes of weanlingpiglets. X. Wang* 1 , Y. Feng 1 , G. Shu 1 , Q. Jiang 1 , J. Yang 1 , and Z. Zhang 2 ,1College of Animal Science, South China Agricultural University, Guangzhou, Guangdong Province, PR China, 2 Zhengzhou Newwill Nutrition TechnologyCo., Ltd., Zhengzhou, Henan Province, PR China.The experiments were conducted to investigate the effect of hydrolyzed wheatgluten on growth performance, cell immunity and serum biochemical Indexesof weaned piglets. 120 crossed Large White × Landrace weanling piglets withan average initial body weight of 10.49 ± 0.02 kg, were randomly allocated tofour dietary treatments. There were three replicate pens per treatment and pigswere grouped with 10 piglets per pen. The experiment lasted 28 d. The dietarytreatments were: (1) basal diet, (2) basal diet + 3% hydrolyzed wheat gluten, (3)basal diet + 5% hydrolyzed wheat gluten, and (4) basal diet + 0.25% Glycyl-Glutamine (0.25% Gly-Gln). All data were analyzed with SAS (SAS Institute,Cary, NC, USA) using ANOVA. The results showed that the supplement ofhydrolyzed wheat gluten in basal diets improved the average daily gain, G:Fratio, decreased diarrhea occurrence and the percentage of CD 3+T cells inblood, increased and CD 4+/CD 8+ratio. At the 14th and 28th day, pigs fed withhydrolyzed wheat gluten had significantly higher albumin/globulin (A:G) ratiothan those fed with the basal diet. At the 28th day, compared with the controlgroup, pigs fed with 3% wheat gluten gluten had increased T, B lymphocyteproliferation in blood. The supplement of 0.25% Glycyl-Glutamine in basaldiet could decrease diarrhea occurrence compared with the control group. Atthe 14th and 28th day, there was no significant difference in glucose, serumurea nitrogen, and triglyceride in serum among these treatments. These resultssuggested that hydrolyzed wheat gluten may stimulate growth performanceby strengthening cell immunity and decreasing diarrhea occurrence. Optimumsupplemental level of hydrolyzed wheat gluten in diet for weanling piglets was3%.The work was supported by National Natural Science Foundation of China(30671519); The Joint Funds of the National Natural Science Foundation ofChina (U0731004); National Science & Technology Pillar Program During the11th Five-year Plan of China (2006BAD12B06); and the Major Program ofGuangdong Science and Technology (2009A080303009).Key Words: hydrolyzed wheat gluten, weanling piglet, growth performanceM154 Effects of copper loaded chitosannanoparticles on growth, immunity and antioxidant activity inweaned pigs. M. Wang* 1,2 and X. Xie 1,2 , 1 Animal Science Collegeof Zhejiang University, Hangzhou, China, 2The Key Laboratory ofMolecular Animal Nutrition, Ministry of Education, Hangzhou, China.The objective of this study was to evaluate the effects of copper loadedchitosan nanoparticles (Cu-CNP) on growth, immunity and antioxidant activityin weaned pigs. One-hundred-eight weaned pigs with an initial average bodyweight 7.20 ± 0.81 kg were blocked by body weight and sex, and randomlyassigned to six treatments with three replicates. Pigs were offered one of sixdiets including a control diet or the control diet supplemented with 100 mg/kgchlorotetracycline (CTC), or 50, 100, 150 and 200 mg/kg Cu-CNP respectivelyfor 28 d after being pretested for 5 d. Upon termination of the feeding trial,blood was collected from the anterior vena cava for serum parameters analyses.The results showed that the CTC and 50 mg/kg Cu-CNP significantly improvedADFI and ADG (P < 0.05), and decreased the feed:gain ratio (F/G) (P < 0.05),but no significant difference was found in ADFI, ADG and F/G between the CTCand 50 mg/kg Cu-CNP treatment (P > 0.05). Compared with the control group,supplementation of 50 mg/kg Cu-CNP significantly increased the contents oftotal protein (TP), albumin and lysosome activity, and C3,GSH-Px level inserum (P < 0.05). And serum urea nitrogen (SUN) in the CTC and Cu-CNPtreated groups were significantly decreased (P < 0.05). Besides, the contentsof TP and SUN have significant difference between the CTC and 50 mg/kgCu-CNP treatment (P < 0.05). These results suggest that Cu-CNP has beneficialeffects on growth, immunity and antioxidant activity in weaned pigs.Key Words: copper loaded chitosan nanoparticles, weaned pig, growthM155 Effects of different dietary lysine levels on apparentnutrient digestibility and serum amino acid concentration and serumbiochemical indexes in growing pigs. P. Zeng, C. Zhang, X. Wang*, Y. Feng,and C. Zhu, College of Animal Science, South China Agricultural University,Guangzhou, Guangdong Province, PR China.This paper was conducted to study the effect of different dietary lysine levelson apparent digestibility of nutrients and serum amino acid concentration andserum biochemical indexes in the growing pigs. The trial was used the totalcollection method. Twelve cross-bred castrates (Large White × Landrace) atabout 20 kg BW were randomly allotted to three treatments on the basis of initialweight and size, 4 replicates of 1 pig each. One pig stayed in one metaboliccage, experiment consisted of a 4-d adjustment phase and a 6-d collecting phasewith experimental diets. Pigs could feed and water ad libitum. The dietarytreatments were: (1) basal diet containing 0.65% lysine (treatment 1); (2) basaldiet, adding Crystalline L-lysine-HCL, containing 0.95% lysine (treatment 2);(3) and diet containing 1.25 % lysine (treatment 3). The differences among thegroups were determined by Duncan's multiple-range test. The results indicatedthat the apparent digestibility of dietary nutrients of treatment 2 was best. Theapparent digestibility of energy, dry matter, crude protein and phosphorus of38