Inaugural ASASâCAAV Asia Pacif ic Rim Conference Abstracts

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pressure of low protein diet because they have been traditionally raised underlow protein diet, we investigated the effect of maternal protein restriction duringpregnancy and lactation on offspring performance and GR expression in varioustissues. Indeed, maternal protein restriction modified offspring GR expression inhippocampus, liver and muscle, which is accompanied by alterations in growthand metabolism. Moreover, both mRNA and protein contents of DNMT1 inhippocampus and liver were found to be different between breeds and alsoaffected by maternal protein restriction, implying that epigenetic mechanismmay be involved in the formation of breeds and metabolic programming.51 Temporal proteomics analysis reveals continuous impairmentof intestinal development in neonatal piglets with intrauterine growthrestriction. X. Wang* 1 , W. Wu 1 , G. Lin 1 , D. Li 1 , G. Wu 1,2 , and J. Wang 1 , 1 StateKey Laboratory of Animal Nutrition, China Agricultural University, Beijing,China, 2 Department of Animal Science, Texas A&M University, College Station,Texas, USA.Efficiency of nutrient utilization is reduced in neonates with intrauterinegrowth restriction (IUGR) compared with those with a normal birth weight(NBW). However, the underlying mechanisms are largely unknown. In thisstudy, we applied temporal proteomic approach, coupled with histological andbiochemical analyses, to study dynamic changes of the proteome in the smallintestinal mucosa of IUGR piglets during the nursing period (d 1, 7, and 21).Eighteen litters of piglets were spontaneously delivered from first-parity sows.At birth, 1 IUGR piglet (≈0.7 kg) and 1 NBW piglet (≈1.3 kg) were obtainedfrom each of 18 litters. The selected piglets (n = 36; 18 IUGR vs. 18 NBW)were positioned in the second teat pairs sucking milk from their own motherfor 21 days. On d 1, 7, and 21, neonatal piglets (6 IUGR and 6 NBW) fromeach of 6 litters were weighed and then killed for weight and length tests ofsmall intestine, coupled with histological, biochemical and proteomic analysesof small intestinal mucosa. During the nursing period, the weight and lengthof IUGR small intestine were continuously lighter and short respectively,whereas the small intestinal mucosa of IUGR suffered a severe impairment.Furthermore, we identified 56 differentially expressed protein spots betweenIUGR and NBW piglets. These proteins participate in key biological processes,including 1) absorption, digestion, and transport of nutrients; 2) cell structureand motility; 3) glucose and energy metabolism; 4) lipid metabolism; 5) AAmetabolism; 6) mineral and vitamin metabolism; 7) cellular redox homeostasis;8) stress response; and 9) apoptosis. The results of our temporal proteomicsanalysis revealed continuous impairment of intestinal development in neonatalpiglets with IUGR.Key Words: intrauterine growth restriction, piglets, intestinal development52 Effects of L-arginine on growth performance, nutrientdigestibility and absorptivity, and blood biochemical parameters inartificially reared neonatal piglets. Lin Huang* 1,2 , Zongyong Jiang 1 , ChuntianZheng 1 , Shouqun Jiang 1 , and Xianyong Ma 1 , 1 Key Laboratory of AnimalNutrition and Feed (South China), Ministry of Agriculture of P. R. China,Guangdong Public Laboratory of Animal Breeding and Nutrition, Institute ofAnimal Science ,Guangdong Academy of Agricultural Sciences, Guangzhou,Guangdong, P.R.China, 2 College of Animal Science, South China AgriculturalUniversity, Guangzhou, Guangdong, P.R.China.This study investigated the effects of dietary L-arginine supplementation onthe growth performance, the ability of digestion and absorption, and bloodbiochemical parameters of artificially reared neonatal piglets. Thirty-six neonatalpiglets at 4 d of age were randomly divided into 3 groups supplemented with0% (control), 0.4, and 0.8% L-arginine, respectively (12 pigs per group). At d11 and 18 of the experiment, 4 piglets from each group were randomly selectedand slaughtered for blood sample collection. The feces and urine samples werecollected to determine the nutrient digestibility at d 15 to 17. At d 19, bloodsamples were collected after D-xylose was orally administrated to the animalsfor 2 h at the dose of 1 mL/kg of BW. Compared with the control group, dietarysupplementation with L-arginine, especially the 0.4% L-arginine treatment (totalarginine 1.12%), tended to elevate BW and ADG. Dietary supplementation with0.4 and 0.8% L-arginine increased (P < 0.05) serum T3 content at d 11 and 18.Dietary L-arginine supplementation at the 0.4% level decreased (P < 0.05) ureanitrogen concentration at d 11. Plasma arginine concerntrations of piglets with0.4 and 0.8% L-arginine at d 18 increased (P < 0.05) by 77.32 and 89.24%,respectively. The 0.4 and 0.8% L-arginine groups had 2 and 1.4 times highercontents of xylose (P < 0.05) than the control group, respectively. The activitiesof maltose and lactose in jejunum mucosa were elevated (P < 0.05) by 0.8%L-arginine supplementation at d 18. The addition of 0.4 and 0.8% L-arginineincreased (P < 0.05) the activity of lactose in duodenum and jejunum mucosaat d 11. Collectively, these results suggest that dietary arginine supplementationimproved the protein synthesis as well as intestinal digestibility and absorptivityof neonatal piglets.Key Words: blood biochemical parameter, L-arginine, artificially rearedneonatal pigletNonruminant Nutrition II53 Effects of L-arginine and arginineactivator additive on heat shock protein 70 expressionin liver of weanling piglets. X. Wu* 1 , Y. Gao 1 , L. Wang 2 , X. Zhou 1 ,and Y. Yin 1,2 , 1Key Laboratory for Agro-ecological Processes inSubtropical Region, Institute of Subtropical Agriculture, the ChineseAcademy of Sciences, Changsha, Hunan, China, 2 Hubei Key Laboratoryof Animal Nutrition and Feed Science, Wuhan, Hubei, China.As the most important metabolic organ, the main detoxification site, and theprimary defense barrier, the liver is liable to be injured by weaning stress. Theexperiment was conducted to evaluate the effects of arginine activator additive(AAA) and L-arginine (Arg) on serum amino acids, and heat shock protein 70(HSP70) expression in liver in weaned piglets. Eighty-nine healthy Landrace ×Yorkshire piglets from 12 pens weaned at 21 d (average pen weight 5.56 ± 0.51kg) were randomly divided into 3 treatment groups, supplied with diets witha standard diet (control group), or diets supplemented with AAA at 0.8 g/kg,or Arg at 6 g/kg of feed, respectively. At the age of 28 d, six piglets identifiedas closest in BW to the average within each pen were selected randomly fromeach treatment. Selected piglets were slaughtered for HSP70 expression inliver by real-time PCR, western blot, and immunohistochemistry. Both AAAand Arg decreased liver GSH (P < 0.05). Compared with the control group,HSP70 mRNA in liver was significantly increased in the Arg group (P < 0.05).Also, HSP70 expression increased in liver in the Arg group, compared with thecontrol and AAA groups. These findings suggested that dietary supplementationof AAA could maintain liver health in weanling piglets, and Arg had adverseeffects on liver.Key Words: L-arginine, arginine activator additive, heat shock protein 7054 Effects of dietary L-arginine supplementation on endogenousarginine-synthesizing enzymes and intestinal histomorphology in artificiallyreared neonatal pigs. Lin Huang* 1,2 , Zongyong Jiang 1 , Yingcai Lin 1 , ChuntianZheng 1 , and Xianyong Ma 1 , 1 Key Laboratory of Animal Nutrition and Feed(South China), Ministry of Agriculture of P. R. China, Guangdong PublicLaboratory of Animal Breeding and Nutrition, Institute of Animal Science,Guangdong Academy of Agricultural Sciences, Guangzhou, Guangdong,P.R.China, 2 College of Animal Science, South China Agricultural University,Guangzhou, Guangdong, P.R.China.This study was conducted to investigate the effects of dietary L-argininesupplementation on intestinal mucosa enzyme activities and intestinalhistomorphology in artificial rearing of neonatal pigs. Thirty-six neonatal pigsat 4 d of age were divided into 3 groups supplemented with 0 (control), 0.4,and 0.8% L-arginine, respectively (12 pigs per group with 4 replicates). Fourpiglets from each group were randomly selected and slaughtered for blood andsmall intestine samples at d 11 and 18. Dietary supplementation with 0.8%18
L-arginine increased (P < 0.05) the activity of nitric oxide synthetase in bloodand jejunum mucosa at d 18. The duodenum concentration of nitric oxide inpiglets supplemented with 0.4% L-arginine was generally higher (P < 0.05) thanthat of the other 2 groups at d 11. Dietary supplementation with 0.4% and 0.8%L-arginine increased (P < 0.05) the activities of inducible nitric oxide synthetase,glutamine synthetase, and ornithine decarboxylase in jejunum mucosa at d 18.Dietary supplementation with 0.4 and 0.8% L-arginine decreased (P < 0.05)arginase activity in ileum mucosa and putrescine production in jejunum mucosaat d 11. The concentrations of spermine in jejunum mucosa were increased (P< 0.05) by 0.4% L-arginine supplementation at d 18. At d 11, the villus heightand villus area in duodenum and jejunum were higher (P < 0.05) in pigletssupplemented with 0.8% L-arginine than in unsupplemented piglets but not inileum, respectively. At d 18, the villus height and villus area in ileum were atelevated levels by supplementation with 0.8% L-arginine (P < 0.05). Theseresults suggested that dietary arginine supplementation improved growth andintestine development in piglets by regulating the production of intestinal nitricoxide and polyamine in jejunum, increasing the villus height in intestine ofneonatal piglets and stimulating enzyme synthesis of intestinal mucosa.Key Words: intestinal histomorphology, L-arginine, artificially reared neonatalpig55 Effects of dietary glutamine on growth performance, serumhormone, and intestinal mucosa enzyme activities in artificially rearedneonatal pigs. Zongyong Jiang* 1 , Weichuan Zheng 1,2 , Yingcai Lin 1 , ChuntianZheng 1 , and Lin Huang 1,2 , 1 Key Laboratory of Animal Nutrition and Feed (SouthChina), Ministry of Agriculture of P. R. China, Guangdong Public Laboratoryof Animal Breeding and Nutrition, Institute of Animal Science, GuangdongAcademy of Agricultural Sciences, Guangzhou, Guangdong, P.R. China,2College of Animal Science, South China Agricultural University, Guangzhou,Guangdong, P.R. China.This study was conducted to determine the effects of dietary glutaminesupplementation on growth performance, serum hormone status, and intestinalmucosa enzyme activities in artificially reared neonatal pigs. The neonatal pigsat 4 d of age were selected and divided into 3 groups according to parity andBW. The control group was fed a basal diet, and the other 2 groups were fed thesame basal diet with supplementation of 0.5 and 1.0% glutamine, respectively.At the end of 14 and 21 d, one piglet per replicate was slaughtered; blood andjejunal mucosa were sampled. Dietary supplementation with 0.5 and 1.0%glutamine had no significant influence on serum IGF-1 and INS concentrations,BW at 14 and 21 d of age, ADG at 4 to 14 d of age, and 4 to 21 d of age (P >0.05). The use of 1.0% glutamine increased ADFI at 4 to 14 d of age (P < 0.05).Adding 0.5% glutamine increased the concentrations of gastrin in serum at 14d of age (P < 0.05). Supplemental 1.0% glutamine significantly increased theconcentrations of gastrin in serum at 21 d of age (P < 0.05). Adding 0.5 and1.0% glutamine significantly increased the activity of ornithine decarboxylase injejunal mucosal at 21 d of age (P < 0.05). Supplementation with 1.0% glutaminedecreased the activities of glutamine synthetase and ornithine aminotransferase(P < 0.05) and increased the activities of Ca/Mg-ATPase and Na/K-ATPasein jejunal mucosa at 21 d of age significantly (P < 0.05). Therefore, dietaryglutamine supplementation could improve growth performance and intestinaldevelopment in artificially reared neonatal pigs.Key Words: intestinal mucosa, glutamine, neonatal piglet56 Estimation of the true ileal digestible threonine:lysine ratiofor nursery pigs fed diets containing distillers dried grain with solubles.Z. P. Zhu* 1,2 , R. B. Hinson 1 , L. Ma 1 , D. F. Li 2 , and G. L. Allee 1 , 1 University ofMissouri, Columbia, MO, USA, 2 China Agricultural University, Beijing, P. R.China.Two experiments with 2,011 nursery pigs were conducted to estimate the trueileal digestible threonine:lysine (TID Thr:Lys) ratio for 12 to 24 kg nurserypigs fed diets containing 30% distillers dried grain with solubles (DDGS).In Exp. 1, a total of 1,005 pigs (TR-4 × C22; 16.7 ± 0.2 kg) were allottedto 1 of 6 treatments in a randomized complete block design with 7 replicatepens (20 to 25 pigs/pen) per treatment. Dietary treatments included one cornsoybeanmeal control diet and 5 diets containing 30% DDGS with levels ofTID Thr:Lys ratios of 53, 57, 61, 65, and 69%, respectively. In Exp. 2, a totalof 1,006 pigs (PIC22 × 327; 12.6 ± 0.2 kg) were allotted to 1 of 7 treatmentswith 6 replicate pens (22 to 25 pigs/pen) per treatment. Dietary treatment wasthe same to Exp. 1, except with a lower TID Thr:Lys ratio of 49%. All the dietswere formulated to contain 1.15% TID lysine. Dietary threonine content wasincreased by adding L-threonine. Growth data were collected for 14 and 19 din Exp. 1 and 2, respectively. In Exp. 1, increasing TID Thr:Lys ratio had noeffect (P > 0.05) on ADG and ADFI, but improved (linear, P < 0.05; quadratic,P < 0.05) G:F (0.597, 0.623, 0.624, 0.621, and 0.621, respectively). The DDGSdecreased (P < 0.01) pig ADG (0.671 vs. 0.636 kg/d), with no effect (P > 0.05)on ADFI and G:F. In Exp. 2, increasing TID Thr:Lys ratio improved (linear,P < 0.01; quadratic, P < 0.01) G:F (0.585, 0.623, 0.628, 0.632, 0.618, and0.632, respectively) with no effect (P > 0.05) on ADG and ADFI. The DDGSdecreased (P < 0.01) pig ADG, ADFI, and G:F. In conclusion, a TID Thr:Lysratio of 61% appears optimal for feed efficiency in 12- to 24-kg nursery pigs feddiets containing 30% DDGS.Key Words: nursery pig, distillers dried grain with solubles, threonine57 Effects of lysine and protein intake during two consecutivelactations on subsequent reproductive performance in multiparous sows.F. R. Huang, H. B. Liu, F. Zhang, and J. Peng*, Huazhong AgriculturalUniversity, Wuhan, Hubei, China.The aim of the study was to investigate the effect of lysine and protein intakeduring two consecutive lactations on subsequent reproductive performance inmultiparous sows. Experiment was designed in a 2 × 2 factorial arrangementwith 2 levels of total lysine (0.95 and 1.10%) and 2 levels of CP (17.5 and19.0%). The experimental period are composed of two consecutive lactationsand mediate gestation. The treatment diets were the same for all sows during2 consecutive lactations. The first lactating sows were housed in the halfopenfarrowing room with an average ambient temperature of 26.30 ± 1.68°Cat 0800 h and 30.35 ± 2.76°C at 1400 h, respectively. The average ambienttemperature of the second lactating farrowing room was 24.42 ± 2.18°C at 0800h and 27.33 ± 3.67°C at 1400 h, respectively. Treatment diets did not affectADFI of the first lactating sows (4.71, 5.01, 5.23, and 4.90 kg/d, respectively),and the second lactating sows (6.02, 5.52, 5.95, and 5.65 kg/d, respectively;P > 0.05). Compared with the 17.5% CP groups, 19.0% CP groups increasedthe first and second lactating sow CP intake (P < 0.05). Likewise, the 1.10%lysine diets increased the first and second lactating sow lysine intake than the0.95% lysine diets (P < 0.05). Furthermore, the 17.5% CP and 1.10% lysinediet decreased the first lactating sow BW loss than the 17.5% CP and 0.95%lysine diet (P < 0.05). However, the 1.10% lysine diet increased the secondlactating sow BW loss than the 0.95% lysine diet (P < 0.01). Remarkably, the19.0% CP diets decreased the first lactating sows BW loss than the 17.5% CPdiets (P < 0.05). Over 2 consecutive lactations, the 19.0% CP diets decreasedthe subsequent culling rate compared with the 17.5% CP diets (P < 0.05). Theseresults indicated increasing lysine intake under the low feed intake conditionwas beneficial for reducing lactation weight loss, whereas increasing lysineintake under normal feed intake condition induced lactation weight loss.Key Words: lysine, protein, subsequent reproduction performance58 The difference in distribution of activity and mRNAabundance of oxidation enzymes for conversion of DL-2-hydroxy-4-methylthiobutyrate and D-methionine resulted in similar systemicavailability of L-methionine in piglets. Z. Fang* 1,2 , H. Luo 1 , H. Wei 1 ,F. Huang 1 , Z. Qi 1 , S. Jiang 1 , and J. Peng 1 , 1 College of Animal Science andTechnology, Huazhong Agricultural University, Wuhan, Hubei, China, 2 AnimalNutrition Institute, Sichuan Agricultural University, Ya’an, Sichuan, China.This study was conducted to determine whether the extraintestinal availabilityof amino acid (AA) could be improved by supplying the animal with AAsource, more of which can bypass the intestine. Six barrows (35 d old, 8.6± 1.4 kg), implanted with arterial, portal, and mesenteric catheters, were feda DL-methionine (DL-Met) or DL-2-hydroxy-4-methylthiobutyrate (DL-HMTB)diet once hourly and infused intramesenterically with 1% p-amino hippurate.Arterial and portal blood samples were taken at hourly intervals until 6 h of tracerinfusion, and pigs were then killed for collection of muscle, intestine, liver, andkidney samples. The AA concentration in plasma samples was determined byion-exchange chromatography. The mRNA abundance of D-amino acid oxidase(D-AAOX), D-2-hydroxy acid dehydrogenase (D-HADH), and L-2-hydroxy acidoxidase (L-HAOX) in tissue samples was determined using semiquantitative19
Page 1 and 2: Inaugural ASAS-CAAVAsia Pacif ic Ri
Page 3 and 4: Scientific ProgramTable of Contents
Page 5 and 6: 1 Advanced needle-free injection te
Page 7 and 8: 9 Pig personality, meat quality, an
Page 9 and 10: 17 The contamination and distributi
Page 11 and 12: 25 Genetic evaluations for measures
Page 13 and 14: of control and the lowest of SDAP g
Page 15 and 16: 39 Effects of bacterial protein and
Page 17: Advances in Digestive Physiology Me
Page 21 and 22: average final weight (AFW) and aver
Page 23 and 24: 71 Building a foundation: Cells, st
Page 25 and 26: 78 Effect of the level of vitamin A
Page 27 and 28: 86 Evaluation of phosphorus excreti
Page 29 and 30: 94 Responses of dairy cows to suppl
Page 31 and 32: 102 Construction and analysis of a
Page 33 and 34: M132 Study on the effects of pectin
Page 35 and 36: M140 Effect of Mintrex Zn on perfor
Page 37 and 38: M148 Effect of the hydrolyzed wheat
Page 39 and 40: treatment 1 was significantly lower
Page 41 and 42: M163 The main fatty acid contents i
Page 43 and 44: M170 Zinc requirements of yellow br
Page 45 and 46: M178 Influences of dietary riboflav
Page 47 and 48: M185 Application of an advanced syn
Page 49 and 50: M193 Studies on the effects of oreg
Page 51 and 52: M202 Plasma leucine turnover rate,
Page 53 and 54: 103 Use of natural antimicrobials t
Page 55 and 56: 111 The somatotropic axis in growth
Page 57 and 58: Environmental Impacts of Cattle, Sw
Page 59 and 60: 128 Opportunities for international
Page 61 and 62: Animal Health PostersT211 Locoweed
Page 63 and 64: T219 Stabilization of roxarsone and
Page 65 and 66: Beef Species PostersUrinary purine
Page 67 and 68: T233 The effects of sire and breed
Page 69 and 70:
T242 Ultrastructure of oocyte and e
Page 71 and 72:
T249 Effect of different combinatio
Page 73 and 74:
Forages and Pastures PostersIn vitr
Page 75 and 76:
T263 Effects of leaf meal of Brouss
Page 77 and 78:
T271 The effects of feeding expandi
Page 79 and 80:
Lactation Biology PostersT278 Effec
Page 81 and 82:
Physiology and Endocrinology Poster
Page 83 and 84:
T288 Effect of Aspergillus meal pre
Page 85 and 86:
Poultry Physiology, Endocrinology,
Page 87 and 88:
T301 Observation of the feeding man
Page 89 and 90:
T307 Effect of levels of Yucca schi
Page 91:
T313 Study of lysine requirement of
Page 94 and 95:
energy, 5, 26energy and nutrient di
Page 96 and 97:
protein digestive enzyme, 44protein
Page 98 and 99:
HHai, Y., T222, T248Hai-Ying, Z., T
Page 100 and 101:
Song, X., T223Song, Z. G, M144, T20
Page 102:
102NOTES
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Inaugural ASASâCAAV Asia Pacif ic Rim Conference Abstracts