Inaugural ASAS–CAAV Asia Pacif ic Rim Conference Abstracts

center, National Agriculture Cooperative Federation, and 103 blood samplesfrom progeny of 12 progeny-test bulls collected from farms around Kyongbukprovince area. Each data group was divided into 2 subgroups, on whichthe missing genotypes were included or excluded, to confirm the influences ofmissing genotypes to the accuracy of relationship estimation. Estimations ofrelationships using genomic information were carried out by each chromosomaland whole genomic SNP marker based on the method of VanRaden (2007).The average correlation coefficient between relationships using pedigreeinformation and chromosomal genomic information on data with verifiedparentage test and eliminated missing genotypes was 0.81 ± 0.04 and that whenusing whole genomic information was high of 0.98. Variation of relationshipsbetween noninbred half sibs was 0.22 ± 0.17 on chromosomal and 0.22 ± 0.04on whole genomic SNP markers. The variations were larger and unusual valueswere shown when non-parentage test data were included. Therefore, it wasconsidered that wrong pedigree information was included.Key Words: genomic relationship, genomic selection, single nucleotidepolymorphismT238 The genetic effects of a rare male immigrant to smallpopulations: A laboratory study using Tribolium castaneum. J. M. Liu,Z. Li*, and J. H. Sun, Qingdao Agricultural University, Qingdao, ShandongProvince, China.This study examined the genetic effects of a rare male immigrant to smalllaboratory populations of Tribolium castaneum. The rare males were froma Giant line of Tribolium castaneum, which was established by individualscollected in a farmer wheat storage of Rizhao countryside of Shandong provinceof China and up-selected for body weight for 10 generations, achieving asignificant genetic progress. Each of 10 base small populations was formedby 4 virgin males and 5 virgin females and the control with 5 virgin malesand 5 virgin females randomly selected from a Pygmy line introduced fromChina Agricultural University. The rare male individuals showed a significantlylarger body size than the Pygmy males (P < 0.01). Beetles were raised in 20-mm diameter × 55 mm shell vials containing 10 g of standard medium (95%by weight of fine-sifted whole wheat flour and 5% of dried powdered yeast).Vials were maintained in a dark incubator regulated at 32°C and 70% relativehumidity. After 72 h following immigration, all 10 individuals were removedfrom each vial, which would be kept for the following 23 d to collect pupae,larvae, and possible adults. The genetic effects of immigrants were assessedby comparing the progeny number, pupa weight, and sex ratio in the first andsecond generations following immigration. The results suggested that in thefirst and second generations, average value of pupa weight of both sexes wassignificantly improved for populations with immigrants (P < 0.01); populationswith immigrants produced a significantly higher number of larvae and pupae(P< 0.05) than the control; and there was no significant difference for sexratiobetween populations with immigrants and the control. This study couldhave implications for conservation biology in that those rare males could getadvantages over the resident males in competing for successful mating, andan occational arrival of rare male immigrants may rescue populations fromextinction.Key Words: Tribolium castaneum, immigration, rare male effectT239 Genetic variation of 3′ untranslated region of Mxgenes in Langya chicken breeds by polymerase chain reaction-restrictionfragment length polymorphism. Z. G. Liu, Z. Li*, B. W. Wang, B. Yue, andW. H. Ge, High Quality Waterfowl Research Institute, Qingdao AgriculturalUniversity, Qingdao, Shandong Province, China.Langya chicken is one of the Chinese indigenous chicken breeds, native to theRizhao District of Shandong Province of China. The Mx gene confers resistanceactivity to orthomyxovirus infection and has been found in many organisms,including yeast and vertebrates ranging from fish to humans. In order to explorethe genetic variation of 3′ untranslated region of Mx (myxovirus resistant) genes,141 Langya chickens were detected by PCR-RFLP, which showed 2 genotypesof AA and AB with frequencies of 0.124 and 0.876 (Table 1), respectively, afterPCR products had been cut by Hae III. The observed genotype distributionwas not in Hardy-Weinberg equilibrium for the Mx allele (chi-squared =75.9772, P < 0.01). Besides, the polymorphic fragments were cloned throughEscherichia coli DH5 αand sequenced by TaKaRa company. The sequencingresults revealed a 31-bp deletion at the position of 20771-20802 of Mx genereference sequence (GenBank accession number: NC_006088, DQ788615,DQ788613), which was TTTTAATGATGTATGGGAGACAAAGTTTATA.The experimental results indicated that the breeds were polymorphic and laid afoundation for investigating the associations between genetic variations of Mxgene and disease-resistance in Langya chicken breeds.Table 1. Population genetics of PCR-RFLP for 3′ sequence of Mx genes inLangya chicken breedGenotype NumberGenotypefrequencyAlleleGenefrequencyChisquaredPolymorphisminformationcontentAA 16 0.124 A 0.562 75.9772 0.3711 0.4923 1.6997AB 113 0.876 B 0.438Chi-squared 0.05 = 3.84, Chi-squared 0.01 = 6.63.Key Words: Langya chicken breed, Mx gene, polymerase chain reactionrestrictionfragment length polymorphismT240 The expression characteristic of the MTP gene andthe effect of overfeeding on the expression of MTP in the various tissuesand different developmental stages in Landes geese. Y. Jian-qiang 1,2 andW. Ji-wen* 1 , 1 Key Lab of Animal Genetic Resources, Sichuan, China, 2 SichuanAnimal Science Academy, Sichuan, China.With Landes geese as experimental materials, we adopted the RT-PCR methodto research the expression characteristic of the MTP gene in liver, intestine,brain, pectoralis muscle, hamstring muscles, skin plus subcutaneous adiposetissue, abdominal adipose tissue, and heart at 5 growth points, including 1, 10,13, 14, and 16 wk, and the effect of overfeeding on MTP gene expression inliver and intestine was researched. The results indicated the expression of theMTP gene was detected only in the small intestine at 1 wk; except in the liverand intestinal tissues, the MTP gene was firstly discovered in the brain tissues inthe 10-wk-old geese. The expression level of the MTP gene in liver and intesineincreased dependent on the increase of week of age (P < 0.05). The expressionof the MTP gene was intestine > liver at 1 to 13 wk of age, intestine < liver at13 to 16 wk of age reversely. Overfeeding induced a decrease of expressionof the MTP gene in the liver and an increase in the intestine. Therefore, weinferred that the MTP gene was very important in the fat deposition and transferin Landes geese.Key Words: MTP gene, developmental stages, expressionT241 Study on the molecular evolution and phylogeny ofmitochondrial d-loop sepuence polymorphic in three domestic donkeybreeds of Henan. T. Liu* 1 , L. Zheng 1 , Y. Liu 2 , and S. Zhao 1 , 1 ZhengzhouCollege of Animal Husbandry Engineering, China, 2 Nanjing AgriculturalUniversity, China.Mitochondrial DNA (mtDNA) play an important role in phyletic evolutionand relationship analysis. The mtDNA d-loops were cloned in 49 donkeys of 3breeds: Changhe donkey, Biyang donkey, and Henan small donkey. Nucleotidediversity of the 3 breeds of donkeys is 2.799, 2.295, and 2.163%, respectively.The mtDNA d-loops had high polymorphism in the 3 breeds: the highest was inHenan small donkey, the second was Changhe donkey, and the last was Biyangdonkey. The Biyang donkey had more remote than the Changhe donkey andhad a closer relationship with the Henan small donkey.Key Words: molecular evolution, mitochondrial DNA, donkeyHNe68