Inaugural ASAS–CAAV Asia Pacif ic Rim Conference Abstracts

M136 Effects of zinc glycine chelate on antioxidation,contents of trace elements and intestinal morphology in broilers. H. Niu,Y. Wang, W. Ma, J. Xiong, and J. Feng*, College of Animal Science, ZhejiangUniversity, Hangzhou, China.A total 360 Ross × Ross 1-day old broilers were used to study the effects ofzinc glycine chelate (Zn-Gly) on antioxidation, contents of trace elements andintestinal morphology. All broilers were randomly assigned to 6 treatmentgroups consisting of 3 replicates. Treatments consisted of 0, 30, 60, 90, and120 mg/kg zinc glycine chelate groups and 120 mg/kg zinc sulphate group.On day 21 and 42, 36 birds were slaughtered. Blood, liver, feces and smallintestine were collected. Liver homogenates and serum were analyzed forantioxidant enzyme activity. Serum and feces analyzed for mineral (iron,copper, zinc and manganum) with flame atomic absorption spectrophotometry.Intestinal morphology was observed by light microscopes Data were analyzedby ANOVA as a randomized complete block design using the GLM proceduresof SAS (version 6.02, SAS Institute, Cary, NC, USA). The addition of 90–120mg/kg Zn-Gly led to an improvement of activity of superoxide dismutase andglutathione peroxidase and a reduction of malondialdehyde content in liversfor 0–21 d. The minimum value of malondialdehyde in 22–42 d broilers werefound in the 90 mg/kg Zn-Gly group. Zinc supplementation, as either ZnSO 4orZn-Gly had no effect on the contents of copper, manganese and iron in serumand feces. For 3 wk broilers, supplement of 90–120 mg/kg Zn-Gly significantlyimproved the concentrations of zinc compared to the control. During 22–42 d,adding 120 mg/kg zinc can significantly increase the concentrations of zinc (P< 0.05). Supplementation with 90 mg/kg Zn-Gly increased villus height anddecreased crypt depth and intestinal wall thickness of jejunum. Ileum cryptdepth of the broilers supplemented with 90 mg/kg Zn-Gly showed significantdecrease compared to that of supplemented ZnSO 4(P < 0.05). In conclusion,Zn-Gly could improve intestinal morphology, serum zinc and antioxidantenzyme activities in chicks.Key Words: Zn-Gly, antioxidation, intestinal morphologyM137 Effects of Mintrex Cu on growth performance inbroilers. W. Jinlei, Z. Tianguo, S. Qiujuan, and G. Yuming*, College of AnimalScience and Technology, China Agricultural University, Beijing, China.The objective of this study was to investigate effects of Mintrex Cu (NovusInternational, Inc., St. Charles, MO, USA) on growth performance of broilers indifferent supplemental way. This study was designed to investigate the impactof Mintrex Cu on broiler performance and the experiment lasted for 42 d. Atotal of 168 one-day-old male AA broiler chicks were allotted to 3 treatmentsin a completely randomized design with 8 replicates per treatment and 7 birdsper replicate pen. The control is supplemented with inorganic minerals fromsulfate source, i.e. Cu 10 ppm, Zn 50 ppm and Mn 60 ppm separately. Group2 was basal feed supplemented with Cu 5 ppm from sulfate and 5 ppm fromMintrex Cu, and Group 3 was basal diet supplemented with Cu 10 ppm fromMintrex Cu. The diet was corn and soybean-based diet and formulated basedon NRC (1994). The trace mineral elements were Cu 8 ppm, Mn 17.5 ppm, andZn 22.5 ppm, respectively, based on the actual analysis of the minerals in thefeed ingredients. All diets were isoenergetic, isonitrogenous and isomethoine,part of the Met source was provided from Mintrex for Mintrex treatments(Mintrex provide about 80% Met activity). The results showed that MintrexCu supplementation did not influence FI, BW gain, FCR and mortality of thebroiler chickens. Highest phalanx and tibia Cu concentrations were observedin Group 2 (P < 0.05), and those birds in the group also had high hepaticCuZn-SOD activity. There was no significant difference between the controland Group 3 fed with Cu at 10 ppm. Replacement of Mintrex Cu for sulfiteCu didn’t influence the blood lymphocyte proliferation exposed to ConA orLPS. The BSA antibody production 10 d postprimary injection of BSA wasnumerically increased in the chickens fed with Mintrex Cu (0.05 < P < 0.10),however no difference was observed among the groups on day 10 post-secondinjection of BSA.M138 Effects of Mintrex Zn on growth performance inbroilers. W. Jinlei, Z. Tianguo, S. Qiujuan, and G. Yuming*, College of AnimalScience and Technology, China Agricultural University, Beijing, China.The objective of this study was to investigate effects of Mintrex Zn (NovusInternational, Inc., St. Charles, MO, USA) on growth performance in broilers indifferent supplemental ways. This study was designed to investigate the impactof Mintrex Zn on broiler performance. The experiment lasted for 42 d. A totalof 168 one-day-old male AA broiler chicks were allotted to 3 treatments in acompletely randomized design with 8 replicates/treatment and 7 birds/replicatepen. The control group is the inorganic mineral supplemented group (sulfitesource, i.e. Cu 10 ppm, Zn 50 ppm and Mn 60 ppm separately), and Group 2 wasbasal diet supplemented with Zn 30 ppm (ZnSO 4) plus 20 ppm Zn from MintrexZn, and Group 3 was Mintrex Zn (Zn 20 ppm) on the top of basal diet. The dietwas corn-soybean based diet and formulated based on NRC (1994). The tracemineral elements from the basal diet were Cu 8 ppm, Mn 17.5 ppm and Zn22.5 ppm respectively, based on the actual analysis of the minerals in the feedingredients. All diets were isoenergetic, isonitrogenous and isomethoine, partof the Met source was provided from Mintrex for Mintrex treatments (Mintrexprovide about 80% Met activity). The results showed that supplementation ofMintrex Zn at 20 ppm to replace ZnSO 4or add on top didn’t influence FCRof the chickens. Supplemental Mintrex Zn at 20 mg/kg increased FI and BWgain and the beneficial effects of Mintrex Zn were mainly observed during theperiod of 22–42 d. The Zn levels in the sera and liver tissues, Mintreax Zn addon the top resulted in significantly increased high Zn retention in the phalanxand tibia of chickens. The activity of hepatic CuZ SOD was higher in MintrexZn and AKP supplemented groups. Mintrex Zn didn’t significantly affect theblood lymphocyte proliferation exposed to ConA or LPS. The BSA antibodyproduction 10 d post-primary injection of BSA was increased in the chickensfed with Mintrex Zn (P < 0.01).Key Words: Mintrex Zn, broiler, growthM139 Effect of Mintrex Mn on performance and eggshellquality in laying hens. S. Qiujuan, W. Jinlei, Z. Tianguo, and G. Yuming*,College of Animal Science and Technology China Agricultural University,Beijing, China.This study was conducted to assess efficacy of Mintrex Mn (NovusInternational, Inc., St. Charles, MO, USA) on performance of laying hens indifferent supplemental ways. A total of one-hundred-eight 37-wk-old Hylinebrown layers were randomly allotted to 2 treatments with 6 replicates pertreatment (3 pens per replicate with 3 layers each). The control is the basaldiet with inorganic minerals (CuSO 4, ZnSO 4and MnSO 4separately, i.e. Cu 10ppm, Zn 30 ppm and Mn 30 ppm). The second treatment was supplementedwith a combination of Mintrex Mn at 20 ppm and sulfite Mn at 10 ppm. Thediet was formulated based on NRC (1994). The trace minerals in basal dietswere Cu 6.5 ppm, Mn 17 ppm and Zn 20 ppm, respectively, based on the actualanalysis. All diets were isoenergetic, isonitrogenous and isomethoine, part ofthe Met source was provided from Mintrex (Mintrex provides about 80% Metactivity). The experiment lasted for ten weeks from 39- to 48-wk-old. Theresults showed that supplementation of Mintex Mn to replace sulfite Mn 20ppm of 30 ppm didn’t significantly affect FI, BW growth, egg production, orthe egg mass of the laying hens. Egg weight and the eggshell thickness wereincreased by Mintrex Mn and FCR tended to be improved numerically. MintexMn supplementation didn’t influence Mn level in the tissues of sera, phalanxand yolk, but significantly increased the hepatic and pancreas Mn levels at wk4 and spleen Mn at wk 9 post-experiment. The enzyme activity of hepatic Mn-SOD was significantly enhanced by Mintrex Mn. Mintrex Mn has no significantinfluence on humoral and cellular immunity.Key Words: Mintrex Mn, layers, performanceKey Words: Mintrex Cu, broiler, growth performance34