Inaugural ASAS–CAAV Asia Pacif ic Rim Conference Abstracts

M148 Effect of the hydrolyzed wheat gluten on growthperformance, digestive enzyme activity, and intestinal morphology ofweaning piglets. Y. Feng 1 , X. Wang* 1 , G. Shu 1 , Q. Jiang 1 , J. Yang 1 , and Z.Zhang 2 , 1 College of Animal Science, South China Agricultural University,Guangzhou, Guangdong Province, PR China, 2 Zhengzhou Newwill NutritionTechnology Co., LTD, Zhengzhou, Henan Province, PR China.A total of 120 weaning piglets (Large White × Landrace) at an average initialbody weight of 9.9 kg were used in a 10-day growth trial to investigate theeffect of the hydrolyzed wheat gluten on growth performance, activity ofdigestive enzymes, and intestinal morphology of weaning piglets. The pigletswere randomly allocated to 3 treatments, each of which had 4 pens of 10piglets per pen. The dietary treatments were: (1) basal diet, (2) basal diet +3% hydrolyzed wheat gluten, and (3) basal diet + 0.25% Glycyl-Glutamine(0.25%Gly-Gln). Eight randomly selected pigs from each treatment (two pigs/pen) were slaughtered to determine the activity of digestive enzymes, intestinalmorphology and gene expression at the end of the experiment. All data wereanalyzed with SAS (SAS Institute, Cary, NC, USA) using ANOVA. Theresult showed that the supplement of hydrolyzed wheat gluten in basic dietscan improved growth performance, increased the ADFI (P < 0.05), and ADG,decreased diarrhea occurrence and CD 4+/CD 8+ratio. The villus height wasincreased, and the crypt depth decreased in duodenum as compared with thecontrol group. The supplement of 0.25%Gly-Gln in basic diets also increasedADG and ADFI, decreased diarrhea occurrence. The supplement of 3%hydrolyzed wheat gluten or 0.25%Gly-Gln to the diet improved the activitiesof amylase, lipase, and trypsinase in duodenum digesta. The supplement of3% hydrolyzed wheat gluten or 0.25%Gly-Gln to the diet up-regulated mRNAexpression of PEPT1 and ASCT2 in duodenum and jejunum. These resultssuggested that hydrolyzed wheat gluten may stimulate the growth performanceby up-regulating gene mRNA expression, increasing the digestive enzymeactivities, and improving the intestinal morphology.The work was supported by NSFC(30671519); The Joint Funds of theNational Natural Science Foundation of China(U0731004); NationalScience & Technology Pillar Program During the 11th Five-year Plan ofChina(2006BAD12B06); and the Major Program of Guangdong Science andTechnology(2009A080303009).Key Words: hydrolyzed wheat gluten, weaning piglets, growth performanceM149 Effect of iron on the antioxidant system and bloodbiochemical indexes of piglet. D. Xiaowei*, W. Pengpeng, W. Ping, Z. Ruiyu,C. Juan, and Y. Qingqiang, College of Animal Science and Veterinary Medicine,Henan Agricultural University, Zhengzhou, Ahenau, China.In order to investigate the effect of different doses of iron on the antioxidantsystem and blood biochemical indexes of piglets, iron dextran (150 mg/ml) wasused by injection to induce iron-overloaded and iron-deficient. Fifteen 0-d-oldpiglets with the same body weight were randomly assigned to three groups.The piglets in the iron-overloaded, iron-deficient and the control groups wereinjected with 3 mL iron dextran at 450 mg/kg body weight, 3 mL iron dextranat 150 mg/kg body weight, and 3 mL sterile saline solution at the ages of 3and 7 d, respectively. At the age of 7 d, the piglets were killed. The serum,liver and spleen were collected for biochemical analysis. Iron contents in serumand tissues of liver and spleen were analyzed by Z-2000 atom absorptionspectrometer. The contents of CHO, TG, HDL-C, TB, CB, TP, ALB, Glu, SUN,ALT and AKP were determined by HITACHI 7020 automatic biochemicalanalyzer (Hitachi Ltd., Tokyo, Japan). The levels of MDA, GSH-Px, CAT, POD,SDH, XOD and SOD were measured by UNIC 7200 spectrophotometer (UNICApparatus Co., Ltd., Shanghai, China) using commercial reagents (NanjingJiancheng Biotechnology Institute, Nanjing, China). The results showed thatdifferent doses of iron significantly affected the antioxidant system and bloodbiochemical indexes of piglets. Compared with control group, the iron levelsin organs and serum were increased (P < 0.05) in the iron-overloaded group,while decreased (P < 0.05) in the iron-deficient group. The contents of TP, TB,HDL-C, MDA, GSH-Px and POD in serum were obviously increased (P < 0.05)in the iron-overloaded group, and decreased (P < 0.05) in the iron-deficientgroup. The levels of AKP and TG were decreased (P < 0.05), compared withthe control group. The result also indicated that iron had an effect on lipidperoxidation reaction in piglet.Key Words: piglet, iron, antioxidant systemM150 Effects of different antioxidants on retention rateof vitamin A and vitamin E in piglet premix. Z. B. Yang* 1 , W. R. Yang 1 ,S. Z. Jiang 1 , L. Li 1 , and H. Cao 2 , 1 Shandong Agricultural University, Taian,Shandong, PRC, 2 Novus International, Inc., St. Louis, MO, USA.Two experiments were conducted to evaluate retention rate of vitamin A andvitamin E in high Zn and Cu piglet premixes with or without addition ofantioxidants. In Experiment 1, A typical piglet premix was used, containing355,000 IU/kg vitamin A, 433 IU/kg vitamin E, 5200 mg/kg Cu, 4420 mg/kgZn. The premix was kept in a commercial farm for 54 d without any antioxidantsupplementation in winter in North China, and HPLC was used to detect thecontent of vitamins A and E every 9 d. The results showed that the contentsof vitamins A and E in the premix decreased linearly (P < 0.01) as increasedstorage time. The content of vitamins A and E in the premix was only 53.86%and 36.19% left respectively at d 54. In Experiment 2, two kinds of antioxidants,SQ Max and M6 (Novus International, Inc., St. Charles, MO, USA) were usedin the same typical piglet premix as Experiment 1. The treatments were: 1)the control diet (without addition of any antioxidant); 2) the control diet withaddition of SQ M6 (1200 mg/kg); 3) the control diet with addition of SQMax (2000 mg/kg). The premix of all three treatments was packed in blackaluminium foil bags, and kept in an oven at 40°C for 70 d to imitate summerweather conditions North China. The same testing method was used to detectthe content of vitamins A and E every 7 d. The results showed that vitamins Aand E contents in the premix of the three treatments were decreased linearly (P< 0.01) with increased storage time. Compared with the control, the retentionrate of vitamin A and vitamin E in the premix with SQ M6 and SQ Maxsupplementation had significantly improved (Table 1). In conclusion, vitaminsA and E in the premix can be oxidized rapidly with increased storage time, andaddition of single (SQ M6) or complex (SQ Max) antioxidants is beneficial inprotection of oxidation of vitamin A and vitamin E in the premix.Table 1. Retention rate of vitamins A and E in premix with differentantioxidants at 40°C (%) 1Vitamin AVitamin EWeek CTR SQMax M6 CTR SQMax M60 100 100 100 100 100 1001 97.8 97.1 98.4 78.6 83.9 84.12 95.1 91.7 99 79.7 86.0 86.13 92.3 90.7 99.8 66.1 79.8 79.94 91.3 89.8 93.7 57.1 71.6 71.65 81.7 87.8 87.7 62.7 63.8 63.86 65.8 84.0 79.2 63.8 56.5 56.57 71.1 80.6 84.9 54.0 54.6 49.28 76.4 87.3 90.6 41.5 45.2 42.19 69.7 73.9 77.9 33.9 39.5 41.510 49.3 60.5 67.1 32.0 42.3 43.01Retention rate (%) = the detection value at certain days/the initial content ×100Key Words: antioxidant, premix, retention rate of vitamin A and EM151 Effect of different levels of xylo-oligosaccharidessupplementation on growth performance and nutrients utilization ofpiglets. H. S. Huang 1 , S. Zhou* 1 , Z. B. Yang 2 , W. R. Yang 2 , L. Xiao 3 , andX. A. Zhang 3 , 1 Qinghai University, Xining, PRC, 2 Shandong AgriculturalUniversity, Taian, Shandong, PRC, 3 Shandong Longlive Bio-technology Co.,Ltd, Qingdao,Shandong, PRC.Two experiments were conducted to assess the effects of xylo-oligosaccharides(XOS) on nutrients utilization and growth performance of piglets. In Experiment1, a total of four hundred 28-d-old postweaning pigs were randomly assignedto one of 5 dietary treatments with 8 replicates of 10 piglets each. Treatmentsincluded: 1) basal diet (BD, control); 2) BD + 50 mg/kg XOS; 3) BD + 100mg/kg XOS; 4) BD + 200 mg/kg XOS; 5) BD + 100 mg/kg aureomycin(chloroteracycline). The control diet was formulated to meet all nutritionalrequirements based on NRC (1998). Pigs were fed a control diet for 7 d and thenfed the test diets for 35 d. Body weights and feed intakes were measured everyweek to determine the ADG, ADFI and feed:gain ratio (F:G). Mortalities andhealth status were visually observed and recorded daily throughout the entireexperimental period. All the piglets had the similar ADG, ADFI and F:G (P >37