Inaugural ASAS–CAAV Asia Pacif ic Rim Conference Abstracts

M144 The mechanisms of lipid deposition in dexamethasoneexposed broiler chickens (Gallus gallus domesticus) in the late growingstage. Y. L. Cai* 1,2 , Z. G Song 2 , X. H. Zhang 2 , X. J. Wang 2 , H. C. Jiao 2 , andH. Lin 2 , 1 College of Biological Science, Jinan, Shandong, China, 2 College ofAnimal Science, Taian, Shandong , China.Male Arbor Acres chickens (35 d of age, n = 30) were injected with dexamethasone(DEX) or saline for 3 d, and a pair-fed group was included. DEX administrationresulted in enhanced lipid deposition in cervical fat; abdominal fat and thighfat also had an increased trend. DEX injection increased plasma triglyceride(TG), very-low-density lipoprotein (VLDL) and insulin concentration not onlyin fasted status but also in fed status. DEX administration led to higher postheparinlipoprotein lipase (LPL) activity and plasma glucose level in fastedstatus. In fasted status, DEX administration resulted in increased ME activityin liver, liver FAS activity tended to increase in DEX-injected chicken. Butthe FAS and ME activity in DEX chickens had no significant change in fedstatus. DEX injection resulted in enhanced ACC and FAS mRNA levels inliver in fasted status. Compared to pair-fed, ME mRNA level in liver tendedto increase in DEX chickens in fasted status. In fed status, DEX administrationled to enhanced liver ACC and ME mRNA expressions compared to pairfedchickens. DEX administration up-regulated FAS mRNA expression inabdominal fat in fasted status, but the ME and FAS mRNA levels of abdominalfat in fed status were not altered by DEX injection. DEX injection resultedin LPL mRNA expression of abdominal fat in fed status, and in fasted statusLPL mRNA level trended to increase by DEX injection. We also measured themRNA levels of PPARγ and ATGL in abdominal fat in fasted and fed status.Neither of these two genes' mRNA expression was altered by DEX injection.The results suggested that the increased hepatic de novo lipogenesis and in turn,the increased circulating lipid flux contributes to the augmented fat depositionin adipose tissues in DEX-challenged chickens. Up-regulation of LPL mRNAlevel in abdominal fat and increased plasma LPL activity also contribute tothe enhanced fat deposits. The results indicated that glucocorticoids togetherwith the induced hyperinsulinemia should be responsible for the up-regulatedhepatic lipogenesis.Key Words: dexamethasone, fat deposition, de novo lipogenesisM145 Effect of RRR-α-tocopherol succinate on immunityand meat quality in broilers. Z. Xuhui*, Z. Xiang, Z. Yanmin, and W. Tian,Nanjing Agricultural University, Nanjing, Jiangsu, China.The objective of this study was to compare the effect of two esters ofα-tocopherol, all-rac-α-tocopherol acetate (DL-α-TOA) and RRR-αtocopherolsuccinate (D-α-TOS) on immunity and meat quality in broilerchicks. Three-hundred-twenty day-old Arbor Acres broiler were randomlydistributed to 4 treatments, each of which had 8 pens of 10 chicks per pen.Birds in the control group were fed with the diets supplemented with 30 mg/kgDL-α-TOA (control), or basal diet with D-α-TOS supplementation of 10, 30,50 mg/kg (TOS1, TOS2, and TOS3 group), respectively for 42 d. The resultsshowed that, significant positive correlations were observed between dietarysupplemental α-TOS levels and plasma (R 2 = 0.9831, P = 0.2097) or hepatic (R 2= 0.9336, P = 0.3503) α-tocopherol concentrations, and a negative correlationwith plasma (R 2 = –0.9511, P = 0.0207) or hepatic (R 2 = –0.9903, P = 0.0135)MDA levels. The concentrations of IgA for TOS3 (21 d) or IgG for TOS2 (42d) were significantly increased (P < 0.05) by 25.15% and 30.77%, or 18.49%and 19.15%, respectively, as compared with the control and TOS2. Markedenhancement of splenic T and B lymphocyte proliferation occurred in TOS3as compared to the other groups. Furthermore, 30–50 mg/kg dietary α-TOSsupplementation resulted in an increase in the activities of serum T-SOD, GSH-Px, T-AOC and hepatic T-SOD, T-AOC (P < 0.05 or P < 0.01). Furthermore,hepatic ROS levels were decreased significantly (P < 0.05). As for the meatquality, 48-h drip loss and shear force of breast and leg muscle was significantlydecreased in broilers of 30–50 mg/kg dietary α-TOS supplementation group,and also the cooking loss of leg muscle. These results indicated that, 30–50mg/kg dietary D-α-TOS could enhance the immune functions and antioxidantcapacity of broilers, and further the water holding capacity and tenderness,which might result from increased retention of serum and hepatic α-tocopheroland reduction in lipid peroxidation, as evidenced by the decrease in MDA andROS.M146 Effect of different selenium sources on anti-oxidationfunction in geese. B. W. Wang*, N. Wang, X. X. Jiang, P. Sun, and B. Yue,High Quality Waterfowl Research Institute, Qingdao Agricultural University,Qingdao, Shandong Province, China.To explore the effect of selenium yeast on anti-oxidation function in geese, 96one-day-old geese of similar body weight were selected and randomly dividedinto four groups, with three replicates in each group and eight in each replicate(half male, half female). The proportion of selenium in the same basal dietswas 0.30 mg/kg respectively. Group 1 was treated as control and to the dietof the other three groups was added sodium selenite (SS), Se-enriched yeast(SY) and Nano-Se respectively. The whole experiment lasted 9 wk and after9 wk all geese underwent blood and liver sampling. The main results wereas follows: the activity of GSH-PX, T-SOD and T-AOC among three formsof selenium sources both at 4 and 9 w serum and liver were significantlyhigher than the control group (P < 0.05); the content of MDA and H 2O 2wasreduced significantly (P < 0.05) both in 4 and 9 w serum and liver. There wasno difference in the activity of GSH-Px among three forms of selenium sourcesin both 4 w serum and liver (P > 0.05). SY had more significant ability toincrease GSH-Px than SS in both 4 w serum and liver (P < 0.05). SY had moresignificant ability to increase T-SOD than SS in both 4 and 9 w serum and liver(P < 0.05). SY had more significant ability to increase T-AOC than SS in 4 and9 w serum and 4 w liver (P < 0.05). Nano-Se had more significant ability toincrease T-AOC than SS in 9 w liver (P < 0.05). SY had more significant abilityto reduce the content of MDA than SS in both 4 and 9 w serum and liver (P< 0.05). There was no difference in the content of H 2O 2among three forms ofselenium sources in both 4 and 9 w serum and liver (P > 0.05). The main resultsshowed that under the conditions of the experiment, three forms of seleniumcan promote the anti-oxidation function and SY was a better source of seleniumfor geese.Key Words: goose, different selenium sources, anti-oxidation functionM147 Effect of different selenium yeast addition level onanti-oxidation function and fatty-liver production of liver-breeding geese.P. Sun, B. W. Wang*, Z. G. Liu, B. Yue, X. X. Jiang, and N. Wang, High QualityWaterfowl Research Institute, Qingdao Agricultural University, Qingdao,Shandong Province, China.To explore the effect of different selenium yeast addition level on anti-oxidationfunction and fatty-liver production of liver-breeding geese, one hundred 13-wkoldliver-breeding geese were selected and divided into four groups randomly,25 in each group. The first, second and third group were test group and thefourth group was control group. The addition level of selenium yeast in the dietwas 0.25, 0.15, 0.05, 0.0 mg/kg respectively. The trial lasted 35 d and whenthe trial was ended, the anti-oxidation indicators were measured. The resultsshowed that when 0.25 mg/kg selenium yeast was added, the T-AOC in serumwas significantly higher than that of the control group(P < 0.01); the T-SODactivity in serum and liver was higher than that of the 0.05 mg/kg group(P