Inaugural ASAS–CAAV Asia Pacif ic Rim Conference Abstracts

M170 Zinc requirements of yellow broilers from twentytwoto forty-two days of age. Xiaoyan Liu, Zongyong Jiang*, ShouqunJiang, Guilian Zhou, and Fang Chen, The Key Laboratory of Animal Nutritionand Feed Science (South China), Ministry of Agriculture of P. R. China,Guangdong Public Laboratory of Animal Breeding and Nutrition, Institute ofAnimal Science,Guangdong Academy of Agricultural Sciences, Guangzhou,Guangdong, P.R. China.This experiment was conducted to investigate the effect of dietary zinc levelon growth performance, antioxidant capacity, immune function, and zincdeposition in yellow broilers from 22 to 42 d of age. We also estimated optimaldietary zinc concentration for yellow broilers fed a cornstarch-corn-soybeanmeal diet. A total of 1,080 22-d-old male broilers was randomly assigned to 6dietary treatments with 6 replicates and 40 birds within each pen. The groupsreceived the same basal diet supplemented with 0, 20, 40, 60, 80, or 120 mg/kg zinc from zinc sulfate (ZnSO 4•H 2O). The feeding trial lasted 21 d. Theresults showed that diets supplemented with zinc had no effect on the growthof broilers from 22 to 42 d of age (P > 0.05). It was also observed that zincsupplementation significantly increased the activities of GSH-Px, CuZn SOD,and AKP in serum (P < 0.05) and significantly raised GSH content in serum,zinc concentration in serum and tibia, and MT content in serum and liver (P 0.05). Based on AKP activity in serum and the zinc concentration in tibia, theoptimal supplemented zinc level estimated by variance analysis and multiplecomparisons for broiler chicks were 80 mg/kg, but the optimal supplementedzinc levels estimated by NLIN were 83 and 70 mg/kg.Key Words: zinc requirement, antioxidant capacity, yellow broilerM171 Zinc requirements of yellow broilers from fortythreeto sixty-three days of age. Zongyong Jiang*, Xiaoyan Liu, ShouqunJiang, Yingcai Lin, and Xianyong Ma, The Key Laboratory of AnimalNutrition and Feed Science (South China) of Ministry of Agriculture,Guangdong Public Laboratory of Animal Breeding and Nutrition, Institute ofAnimal Science,Guangdong Academy of Agricultural Sciences, Guangzhou,Guangdong, P.R. China.This experiment was conducted to investigate dietary zinc concentrationson growth performance, antioxidant capacity, immune function, and zincdeposition in yellow broilers from 43 to 63 d of age and to estimate the optimaldietary zinc level for yellow broilers fed a cornstarch-corn-soybean meal diet.A total of 1,080 43-d-old male broilers were randomly assigned to 6 dietarytreatments with 6 replicates and 30 birds within each pen. The groups receivedthe same basal diet supplemented with 0, 20, 40, 60, 80, or 120 mg/kg zinc fromzinc sulfate (ZnSO 4•H 2O). The feeding trial lasted 21 d. The results showed thatdiets with added zinc had no effect on the growth of broilers from 43 to 63 dof age (P > 0.05). Zinc supplementation significantly increased the activitiesof GSH-Px, CuZn SOD, and AKP in serum (P < 0.05) and significantly raisedGSH content in serum, zinc concentrations in serum and tibia, and MT contentin serum and the liver (P < 0.05). Supplementation with 40 to 60 mg/kg zincsignificantly elevated thymus index and index of Bursa of Fabricius of broilers at63 d (P < 0.05). Zinc addition had no effect on spleen index, CuZn SOD activityin liver, or tibia ash percentage (P > 0.05). Based on criteria including AKPactivity in serum and zinc concentration in the tibia, the optimal supplementedzinc level estimated by variance analysis and multiple comparisons for broilerchicks was 78 mg/kg, but the optimal supplemented zinc levels estimated byNLIN were 81 and 60 mg/kg, respectively.Key Words: zinc requirements, antioxidant capacity, yellow broilerM172 Effects of feeding solid-state fermented rapeseed mealon performance, nutrient digestibility, intestinal ecology, and intestinalmorphology of broilers. G. Chiang*, W. Lu, X. Piao, L. Gong, and P. A.Thacker, State Key Laboratory of Animal Nutrition, College of Animal Scienceand Technology, China Agricultural University, Beijing, China.This trial was conducted to determine the effects of feeding a diet containingsolid-state fermented rapeseed meal on performance, nutrient digestibility,intestinal ecology, and intestinal morphology of broilers. A liquid culture with5 log cfu/mL Lactobacillus fermentum, Enterococcus faecium, Saccharomycescerevisae, and Bacillus subtilis was mixed in a 1:1:1:1 ratio. A basal substratecontaining 75% rapeseed, 24% wheat bran, and 1% sugar was mixed with theculture in a ratio of 10:3. During a 30-d fermentation, isothiocyanates werereduced from 119.6 to 14.7 mmol/kg. A total of 168 1-d-old male chicks wereassigned to 3 treatments, including a corn-soybean meal control diet and 2experimental diets in which the control diet was supplemented with 10% of thebasal substrate containing unfermented rapeseed or 10% of the basal substratecontaining fermented rapeseed. From d 19 to 21 and d 40 to 42, excreta werecollected for determining digestibility. Digesta from the colon and ceca werecollected to determine the number of lactobacilli and enterobacteria. Duringwhole trial (42 d), the ADG and FCR of birds fed fermented rapeseed weresuperior (P < 0.05) to that of birds fed unfermented rapeseed and did not differfrom the control. On d 42, birds fed fermented rapeseed had higher (P < 0.05)apparent digestibility for DM, energy, and Ca than birds fed unfermentedrapeseed. Digesta from broilers fed the fermented feed had higher (P < 0.05)lactobacilli counts than birds fed the control and unfermented rapeseed on d21 and 42. Fermentation also improved (P < 0.05) villus height and the villusheight:crypt depth ratio in the ileum and jejunum on d 21 and 42. Solidstatefermentation of rapeseed meal improved performance and the intestinalmorphology of broilers and allowed more rapeseed to be fed to broilers,potentially reducing the cost of production.Key Words: broiler, rapeseed, solid-state fermentationM173 Digestibility of broiler feeds containing different levelsof powder and coated sodium butyrate. Y. Zou 1 , Z. B. Yang* 1 , W. R. Yang 1 ,S. Z. Jiang 1 , X. Zhao 1 , and R. Yu 2 , 1 Shandong Agricultural University, Taian,Shandong, P.R. China, 2 Kangdequan Feed Co., Ltd., Hangzhou, Zhejiang, P.R.China.A digestibility trial was conducted to investigate the effects of supplementationwith different sodium butyrate (SB; provided by Kangdequan Feed Co., Ltd.)levels and sources on nutrient utilization in broilers. Fifty-six 42-d-old ArborAcre broilers were randomly divided into 7 treatments. Each consisted of 4replicate cages of 2 chickens with 8 extra birds kept as negative controls. Thecontrol diet without SB and the tested diets were supplemented with powder orcoated SB (100, 200, and 300 mg/kg). Birds had free access to diets and waterduring the pre-experimental and experimental periods. Excreta were collectedover a period of 72 h, and digestibility coefficients for DM, CP, NDF, and GEwere determined using the equation of nutrient digestibility (%) = (NF - NE+ NENC) × 100/NF, where NF = nutrient in feed, NE = nutrient in excreta,and NENC = nutrient in excreta of negative control. Supplementation of SBpositively affected (P < 0.05) digestibility of DM (74.94 vs. 73.09%), CP(63.02 vs. 60.35%), NDF (67.17 vs. 66.02%), and GE (79.09 vs. 78.36%).Increased supply of SB levels increased nutrient digestibility (P < 0.05); highlevels of SB in broiler feed reflected relatively high digestibility, and PSBimproved digestibility of DM, CP, NDF, and GE by 0.52, 0.97, 0.22, and 0.18%,respectively, in broilers as compared with the coated-SB-supplemented group.In conclusion, SB could be used to improve digestibility of DM, CP, NDF, andGE in broilers, which increased with the supply level, and the effect of thecoated SB was more effective than that of powder (uncoated) ones.Table 1. Effects of sodium butyrate on nutrient digestibility in broilersPowder sodium butyrate Coated sodium butyrateSB (mg/kg) 0 100 200 300 100 200 300 SEMDM(%) 73.09 c 73.68 bc 74.95 ab 75.40 a 74.64 b 75.25 ab 75.71 a 0.226CP(%) 60.35 c 61.84 b 62.46 ab 63.31 ab 62.26 b 63.90 ab 64.37 a 0.306NDF(%) 66.02 c 66.30 bc 67.29 ab 67.60 a 66.62 bc 67.37 ab 67.85 a 0.163GE(%) 78.36 b 78.73 b 79.11 ab 79.14 ab 78.67 b 79.40 ab 79.46 a 0.150a-cDifferent superscripts within a row are significantly difference betweentreatments (P< 0.05).Key Words: sodium butyrate, nutrient digestibility, broiler43