Inaugural ASAS–CAAV Asia Pacif ic Rim Conference Abstracts

Small Ruminant PostersEffect of rearing system on rumen development inT305T303lambs. M. A. Norouzian*, R. Valizadeh, A. Nabipour, A. A. Naserian, and A.M. Tahmasbi, Ferdowsi University of Mashhad, Mashhad, Khorasan Razavi,Iran.Study of the effects of leucine on protein synthesis insheep. S. Dan*, S. Hai-Zhou, Z. Cun-Fa, Z. Chun-Hua, L. Sheng-Li, S. Yan,R. Xiao-Pin, and Z. Hai-Ying, Inner Mongolia Academy of Agriculture andAnimal Husbandry, Huhhot, China.The effect of rearing system on rumen development and noncarcasscharacteristics of Balouchi lambs was studied. Twenty-four male lambs wereused in a completely randomized design. Eight lambs remained with theirmothers throughout the experiment (natural rearing; NR), and 16 lambs weredivided into 2 individually housed groups. The group ARWF (artificial rearingwithout forage) was fed a starter without alfalfa and group ARF (arterial rearingwith forage) was fed a starter containing 15% alfalfa. Blood levels of glucose andblood urea nitrogen were not affected by the applied treatments. Concentrationof β-hydroxybutyrate was higher in the artificial rearing systems, whereasNEFA were higher in naturally reared lambs. Neither DNA content and nor cellsize was affected by rearing method, but RNA content and ribosomal capacitydiffered significantly (P < 0.05) between groups. The ARWF lambs had a thickerkeratinized layer than NR lambs, and the NR lambs had a thicker keratinizedlayer than ARF lambs, but other rumen morphological characteristics were notaffect by the groups. Differences between NR and artificial rearing methods(ARWF and ARF) in empty BW and noncarcass organ weight, except stomachweight (% empty BW) and omasum and stomach capacity, were similar. Theresults of this study showed that the NR system caused minimal developmentof the rumen in comparison with the ARWF and ARF methods.Key Words: rearing method, rumen development, lambT304 Effects of chrysanthemum stem-leaf feeding on thegrowth performance and antioxidant ability of growing Hu lambs. H. L.Mao*, J. K. Wang, P. P. An, J. Lin, and J. X. Liu, College of Animal Sciences,Zhejiang University, Hangzhou 310029, Zhejiang, P. R. China.This study was conducted to determine the effect of feeding chrysanthemumstem-leaf (CSL) on the growth performance and antioxidant ability of Hu lambs.Thirty-six growing lambs were assigned to 4 groups of 9 lambs each, and allottedto 3 units of 3 lambs of similar BW. The ratio of roughage to concentrate in allthe diets was 70:30. The proportions of rice straw to CSL in roughage were70:0, 25:45, 45:25, and 0:70 in diets I to IV, respectively. The trial lasted for 72d, with the first 12 d for adaptation. Feeds ingested and refused were recordedevery week, and BW was measured every 2 wk. At the end of the trial, serumbiochemical and antioxidant parameters were determined. With increasing CSLlevel, ADG and serum urea N increased, with a significant difference betweendiets I and IV (P < 0.05; Table 1). Serum catalase and total antioxidant capacitywere significantly higher (P < 0.05) in lambs on diets III and IV than in those ondiet I. Serum glutathione peroxidase activity was highest in animals on diet III(P < 0.05). Compared with that in diet I, serum malondialdehyde was decreasedin diet IV (P < 0.05). In conclusion, inclusion of CSL in the diets could improvegrowth performance and increase the antioxidant ability in growing lambs.Table 1. Effects of chrysanthemum stem-leaf on growth performance andserum biochemical parameters in growing lambsItem I II III IV SEMGrowth performance (g/d)ADG 96 b 115 ab 132 ab 137 a 15.7Feed intake 1481 1617 1547 1549 37.2Serum biochemical parameterTotal protein, g/L 58 59 61 61 1.5Urea N, mmol/L 6.7 b 6.8 b 7.1 b 8.6 a 0.40SOD, U/mL 140 140 145 145 3.9CAT, U/mL 2.7 a 3.2 ab 4.5 a 4.6 a 0.40GSH-Px, U 63 b 64 b 93 b 66 b 3.8T-AOC, U/mL 5.8 b 7.5 ab 7.8 a 7.9 a 0.60MAD, nmol/mL 2.8 a 2.3 ab 2.3 ab 1.8 b 0.19The purpose of this research was to study the effects of leucine on proteinsynthesis in skeletal muscle in sheep through the Western blot technique andthe isotope tracer technique. We added different levels of rumen-protectedleucine to the diet. Twelve sheep were allotted to receive 0 (control), 0.5, 1.0, or1.5 g/d of rumen-protected leucine every day for 15 d. Until d 15, sheep wereadministered a flooding dose of d5-phenylalanine in a jugular vein and werekilled at 90 min to measure the phosphorylation of two key factors (4E-BP1 andp70S6K) in mTOR signaling pathways, rates of skeletal protein synthesis, andmeat quality traits. Sheep treated with the rumen-protected leucine had greaterphosphorylation of 4E-BP1 and p70S6K compared with the control group (P< 0.05). Absolute gray-scale ratios of phosphorylation on 4E-BP1 and p70S6Kwere 0.40 and 0.85% greater, respectively, in leucine-treated (1.0 g/d) comparedwith the control sheep. Sheep treated with the rumen-protected leucine hadgreater rates of protein synthesis in the LM and biceps femoris compared withthe control group (P < 0.05). Absolute protein synthesis rates in the LM andbiceps femoris were 1.38 and 1.12%/d greater, respectively, in leucine-treated(1.0 g/d) compared with control sheep. The results suggest that adding therumen-protected leucine to the diet increased muscle skeletal protein synthesisand was associated with changes in initiating the regulation of biomarkers ofmessenger RNA translation, as evidenced by upregulated phosphorylation ofthe translational repressor eukaryotic initiation factor (eIF)4E-binding protein1 (4E-BP1) and the phosphorylation of the 70-kDa ribosomal protein S6 kinase(p70S6K).The meat was safe because the meat pH, water loss rate, cook rate,meat color, and tenderness were not significantly difference among the groups.Key Words: rumen-protected leucine, protein synthesis, mTORT306 Study of the ideal pattern of absorbable amino acidsin the small intestine of Aohan fine wool sheep. L. Zhi-You* 1,3 , S. Hai-Zhou 2 ,Z. Cun-Fa 2 , L. Zhi-Ming 3 , Z. Ze-Jun 3 , L. Sheng-Li 2 , S. Yan 2 , Z. Chun-Hua 2 , andL. Shu-Li 2 , 1 College of Animal Science and Animal Medicine, Huhhot, China,2Inner Mongolia Academy of Agriculture and Animal Husbandry, Huhhot,China, 3 Chifeng Institute of Animal Science, Chifeng, China.The objective was to obtain an ideal absorbable AA pattern in the small intestineof Aohan fine wool sheep that would be conducive to the growth of wool. Ninewethers of Aohan fine wool sheep (35 to 45 kg) with the same father, fittedwith permanent ruminal cannulas at the proximal duodenum and terminalileum, were used in the experiment. Three intestinal absorbable AA patterns(M100, M85+H15, M70+H30, where M represents muscle and H representshair), which were established according the AA profiles of the muscle and hairprotein, were analyzed. The AA flow and digestibility in different sites weredetermined by the continuous perfusion technique. A dynamic addition modelwas then used to calculate the infusion doses of AA for different patterns, andthe concentrations of free AA in plasma were measured. Wool growth rate andfineness were measured in sheep on d 28 of the basal diet, under 3 patterns.The sulfur-containing AA flow and the disappearance of AA in the smallintestine were smaller. Among the 3 patterns, the ratio of Gly and total AA(except Gly) of M70+H30, and the concentrations of ALT and plasma ureanitrogen of M70+H30 were better than for the other 2 groups. The infusionof AA significantly increased the growth rate of wool. The wool growth rateof M70+H30 was 871.76 μm/d and was 3.5 times the growth rate of sheep onthe basal diet. We conclude that improving the ideal absorbable AA patterncould significantly increase the wool production performance of sheep. Theideal absorbable AA pattern of Aohan fine wool sheep was close to M70+H30,which is conducive to the growth of wool.Key Words: Aohan fine wool sheep, ideal amino acid pattern, capability ofwool productiona,bSignificant at P < 0.05.Key Words: chrysanthemum stem-leaf, lamb, antioxidant ability88