74 The optimal culture conditions in vitro for bovine mammaryepithelial cells. X. Y. Li, J. Q. Wang*, H. Y. Wei, D. P. Bu, H. Hu, and L. Y.Zhou, State Key Laboratory of Animal Nutrition, Institute of Animal Science,Chinese Academy of Agr<strong>ic</strong>ultural Sciences, Beijing, China.The objective of this study was to optimize the in vitro culture conditions forbovine mammary epithelial cells (BMEC). The inoculation concentration,medium, and the freeze preservation of BMEC were studied in culture conditionby cell proliferation, wh<strong>ic</strong>h was evaluated by cell count trypan blue dying. TheBMEC were isolated from the mammary gland tissue of a 3-yr-old lactating (ca.100 d in milk) Chinese Holstein dairy cow. The epithelial cells were inoculatedin 24-well plates and incubated in the basal medium containing 10% fetalbovine serum (FBS), at 38°C in 5% CO 2incubator, each experiment with 8 dand each treatment with 3 repl<strong>ic</strong>ates. The results of inoculation concentration0.5 × 10 4 , 1 × 10 4 , 2 × 10 4 , and 4 × 10 4 /mL of the epithelial cells ind<strong>ic</strong>atedthat 1×10 4 cells status in each well was the best mode. The cell growth cycleexperienced latent phase, logarithm<strong>ic</strong> growth phase, stable phase, and declinegrowth stages, and the growth curve accorded with the S-type growth law.Media Dulbecco’s modified Eagle’s medium (DMEM), F12, RPMI-1640, andDMEM/F12 were used to culture BMEC to assess the effect of the medium onnormal growth of cells. The morphology of epithelial cells cultured in DMEM/F12 appears to be characterist<strong>ic</strong> cuboidal and cobblestone, the growth curveof cells was up to the general biolog<strong>ic</strong>al laws, and the cell growth was thebest. The growth of cells cultured in DMEM was slower than in DMEM/F12,followed by RPMI-1640 and then F12. The serum-freezing solution (90% FBS+ 10% dimethyl sulfoxide) and medium-freezing solution (70% medium +20%FBS +10% dimethyl sulfoxide) were used to preserve the bovine mammaryepithelial cells. The cell proliferation and morphology of serum-frozen cellswere better than the medium-frozen cells. The number of serum-frozen cells wassignif<strong>ic</strong>antly higher than medium-frozen cells from the third day (P < 0.05). Thepresent study supplies some data for the method of culturing BMEC in vitro.Key Words: bovine mammary epithelial cells, culture in vitro, growth curve75 Study on foundation and appl<strong>ic</strong>ation of system to preservemilk good flavor in raw cow’s milk production. Z. Jun* 1,2 , L. Dexun 1 , andG. Min 1 , 1 Animal Nutrition Institute, Inner Mongolia Academy of Agr<strong>ic</strong>ulturaland Animal Sciences, Huhhot, China, 2 College of Animal Science and AnimalMed<strong>ic</strong>ine, Inner Mongolia Agr<strong>ic</strong>ultural University, Huhhot, China.A trial was conducted to study foundation and appl<strong>ic</strong>ation of a HACCP systemto prevent bad milk flavor, and to preserve good milk flavor, in raw cow’s milk.Using lactating cows (n = 500) raised in a dairy farm in the suburb of Huhhot,this trial included 2 periods. In the first 30 d, all cows were raised accordingto the farm ordinary feeding and management regimen. In the remaining 60d, the feeding and management regimens were all changed to the new onesdesigned according to the HACCP system. At 10, 20, 30, 50, 70, and 90 d,milk samples were collected for the analysis of volatile flavor compounds usingpurge and trap-gas chromatography/mass spectrometry. Sensory and flavorintensity index (FII) evaluation in the milk samples taken at different samplingpoints was carried out. All data were statist<strong>ic</strong>ally analyzed by independentsample t-test using SPSS 11.5 for Windows. The results showed that, after theimplementation of the HACCP system, the degree of acidity reduced slightlyand antibiot<strong>ic</strong> index improved, but there were no signif<strong>ic</strong>ant differences (P >0.05). Total number of bacteria were signif<strong>ic</strong>antly lower (P < 0.05), and milkyields were signif<strong>ic</strong>antly higher (P < 0.05). At different sampling points,volatile flavor compound differences in terms of quantity, type, and contentwere found. Sensory evaluation scores increased slightly, but there were nosignif<strong>ic</strong>ant differences (P > 0.05). A new comprehensive evaluation systemwas established, at different sample points where FII was more than before theimplementation of the HACCP system. Flavor intensity index was calculatedas following: FII = Σ(FI1 × A1)/Σ(FI2 × A2), where FI1 = flavor intensity ofgood flavor compounds; A1 = proportion of good flavor compounds in the totalgood flavor compounds; FI2 = flavor intensity of bad flavor compounds; A2 =proportion of bad flavor compounds in the total bad flavor compounds (Lu et al.,2009). In conclusion, the HACCP system seemed to preserve good milk flavorin raw cow’s milk and improved not only good flavor but also milk quality.Key Words: raw milk, HACCP system, volatile flavor compound76 Effects of rumen-protected methionine on dairy performanceand amino acid metabolism in lactating cows. W. R. Yang*, H. Sun, Z. B.Yang, Q. Y. Wang, and F. X. Liu, Department of Animal Science and Technology,Shandong Agr<strong>ic</strong>ultural University, Tai-an, Shandong, P.R. China.An experiment was conducted to study the effect of different levels of rumenprotectedmethionine (RPMet) on dairy performance and serum amino acidmetabolism in lactating dairy cows. Thirty-six Holstein cows in similar conditionwere assigned randomly to 6 experimental treatments with 6 repl<strong>ic</strong>ates each.The adaptation period was 14 d followed by an experiment period of 35 d.Supplementation of rumen-protected methionine in the experimental treatmentswas 0 (control), 14, 28, 42, 56, and 70 g/d per cow. All the data were analyzedusing the GLM procedure of SAS. The results demonstrated that milk yieldof cows fed 42 g of RPMet was signif<strong>ic</strong>antly higher than that of the controlgroup; however, the other groups had no signif<strong>ic</strong>ant difference compared withthe control group in milk yield. Moreover, milk fat percentage was signif<strong>ic</strong>antlyincreased by 56 g of RPMet supplementation. However, the percentage ofmilk protein, lactose, and SNF had no signif<strong>ic</strong>ant difference among treatments.Rumen-protected methionine tended to increase the concentration of serummethionine. Serum EAA contents of the group supplemented with 42 g ofRPMet were lowest, although there was no signif<strong>ic</strong>ant difference among alltreatments. Serum BCAA concentrations of cows fed 28 g of RPMet weresignif<strong>ic</strong>antly lower than that of the control group. Supplementation of 42 gof RPMet could signif<strong>ic</strong>antly decrease the concentration of NEAA and TAAcompared to the control group. In conclusion, supplementation of RPMetimproved dairy performance and promoted amino acid utilization in lactatingcows and 42 g/d of RPMet per cow was suitable in the present experiment.Key Words: rumen-protected methionine, dairy performance, amino acidmetabolism77 Effects of supplementing rapeseed on milk performance andconjugated linole<strong>ic</strong> acid of milk in grazing yak (Bos grunniens). Z. Xiaoling1,5 , H. Li-zhuang 1,2 , H. Jin-suo 1,2 , W. Ke-xuan 1,2 , C. Sha-tuo 1,3 , L. Shu-jie* 1,2 ,W. Jia-qi 4 , and B. Deng-pan 4 , 1 The Academy of Animal and Veterinary Sciencesof Qinghai University, Xining, Qinghai, China, 2 Qinghai Plateau Yak ResearchCenter, Xining, Qinghai, China, 3 Key Laboratory of Plateau Grazing AnimalNutrition and Feed Science, Xining, Qinghai, China, 4 Institute of AnimalSciences, Chinese Academy of Agr<strong>ic</strong>ultural Sciences, Beijing, China, 5 Collegeof Animal Science of Tarim University, Alaer, Xinjiang, China.Effects of feeding rapeseed to grazing yak on milk performance and milk fattyacids, especially CLA, was studied at pasture. Fifteen healthy calving Datongyak cows were randomly blocked into 3 groups, each with 5 cows, and wereassigned into 1 of the following 3 treatments: (1) control: pasture, no supplement,N group; (2) low-level treatment: feeding 100 g/d of rapeseed per cow, L group;and (3) high-level treatment: feeding 200 g/d of rapeseed per cow, H group. Theexperiments were conducted in turning green period (2007/5Ã6), green grassperiod (2007/8Ã9), and grass yellowing period (2007/10Ã11) separately, andeach period lasted 5 wk. The rapeseed was prepared with heating and a highpressureprocess(127°C, steam pressure 117 kPa) and was used as the only dietto regulate the milk constituents. The results ind<strong>ic</strong>ated the following things.(1) In 3 periods, milk yields were not different among treatments, except theH group was enhanced signif<strong>ic</strong>antly (P < 0.05) in the turning green period.Feeding rapeseed showed no effect on the content of total milk constituentsexcept for milk fat content, wh<strong>ic</strong>h was signif<strong>ic</strong>antly lower in the H group thanin the control and L group (P < 0.05) in the green grass period and in the grassyellowing period. (2) Feeding rapeseed decreased the content of short- andmedium-chain fatty acids but improved the unsaturated fatty acid content insupplemented treatment groups. (3) C16:0, C18:0, and c-9,C18:1 were the mostabundant fatty acids of milk fat in grazing yak cows. The content of CLA inmilk fat in control, L, and H group was 12.27, 11.54, and 11.76; 1.53, 1.55, and1.67; and 1.03, 1.00, and 1.06 g/100 g of TFA in in the turning green period,green grass period, and grass yellowing period, respectively. (4) There were nodifferences in the CLA content and the Δ-9 desaturase index among the N, L,and H group, but the CLA content of milk fat was signif<strong>ic</strong>antly higher in thegreen grass period than the other 2 periods (P < 0.05). (5) The proportion ofc-9,t-11 CLA was highest, wh<strong>ic</strong>h comprised over 95% of the amount of totalCLA. In conclusion, feeding rapeseed 200 g/d did not signif<strong>ic</strong>antly enhance theCLA content of milk fat of grazing yak.Key Words: grazing yak, conjugated linole<strong>ic</strong> acid, rapeseed24
78 Effect of the level of vitamin A on growth and biochem<strong>ic</strong>alindexes of growing layer ducks. Y. D. Zhang*, J. L. Wu, and A. Wang, Instituteof Animal Nutrition, Northeast Agr<strong>ic</strong>ultural University, Harbin, China.This study was designed to evaluate the effect of vitamin A on the biochem<strong>ic</strong>alindexes of serum and organization in growing layer ducks. Two hundred sixtyJin-Ding ducks, with an average weight of (0.37 ± 0.01) kg, were chosenand randomly divided into 6 treatments, wh<strong>ic</strong>h were fed a basal diet withsupplements of 0, 2,250, 5,500, 8,250, 12,000, or 15,000 IU/kg of vitaminA, respectively. The trial lasted 7 wk. The results showed that GSH-Px andSOD activities of serum and liver were increased (P < 0.05) with increasingvitamin A levels. The T-AOC of serum and liver were increased signif<strong>ic</strong>antly (P< 0.05), and serum and liver MDA concentration were decreased signif<strong>ic</strong>antly(P < 0.05) when adding 5,500 and 8,250 IU/kg of vitamin A. When adding12,000 and 15,000 IU/kg of vitamin A, T-AOC of serum and liver and GSH-Px and SOD activities of serum and liver decreased compared with adding5,500 and 8,250 IU/kg of vitamin A. It is concluded that duck will evidentlyincrease antioxidant ability and decrease lipid peroxidation when adding 5,500and 8,250 IU/kg of vitamin A. Supplementing with 8,250 IU/kg of vitamin Asignif<strong>ic</strong>antly affects spleen (P < 0.05), ALB (P < 0.05), and GLB (P < 0.01).The content of ALB, GLB, and A/G were not signif<strong>ic</strong>antly different (P > 0.05)when adding 5,500, 8,250, 12,000, or 15,000 IU/kg of vitamin A. The contentof IgG, IgM, and IL-2 first increased then decreased as the level of vitaminA increased (P > 0.05). Vitamin A had no signif<strong>ic</strong>ant effect on the content ofT3, T4 (P > 0.05), but signif<strong>ic</strong>antly affected the content of GH (P > 0.05).It is concluded that the immune function of growing ducks will be improvedand thyrine, chondrotrop<strong>ic</strong> hormone will be regulated when adding 5,500 or8,250 IU/kg of vitamin A. It is suggested that the optimal supplement amountof vitamin A was from 5,500 to 8,250 IU/kg under the cage environment.Note: this research is supported by National Natural Science Foundation ofChina (NSFC, No. 30571345).Key Words: vitamin A, growing layer duck, biochem<strong>ic</strong>al index79 Effect of the level of vitamin E on growth and organismbiochem<strong>ic</strong>al indexes of growing ducks in cages. J. L. Wu*, Y. D. Zhang,and A. Wang, Institute of Animal Nutrition, Northeast Agr<strong>ic</strong>ultural University,Harbin, China.Nonruminant Nutrition III80 Effects of dietary vitamin level on the productive performanceof laying hens. H. Zang* 1 , K. Zhang 1 , X. Ding 1 , J. M. Hernández 2 , and D. Yao 3 ,1Institute of Animal Nutrition, Key Laboratory for Animal Disease-ResistanceNutrition of China Ministry of Education, S<strong>ic</strong>huan Agr<strong>ic</strong>ultural University,Yaan, S<strong>ic</strong>huan 625014, P. R. China, 2 DSM Nutritional Products Ltd., R&DAnimal Nutrition and Health, Wurmisweg 576, CH-4303 Kaiseraugst,Switzerland, 3 DSM (China) Limited, No. 476, LiBing Road, Zhangjiang High-Tech Park, PuDong Area, ShangHai 201203.The trial was conducted to study the effects of dietary vitamin level changeon the laying performance of commercial laying hens after 39 wk of feedingwith different vitamin levels. During 1 to 39 wk, a single factorial design wasused with 4 vitamin levels: treatment 1 was the current average industry levelin China (local); treatment 2 was optimum vitamin nutrition level (OVN);treatment 3 was NRC (1994) with Hy.D (25-hydroxy-cholecalciferol); andtreatment 4 was NRC. A total of 1,800 Lohmann Pink-shell commercial layersat 25 wk of age were assigned randomly into 4 treatments with 10 repl<strong>ic</strong>atesper treatment and 45 layers per repl<strong>ic</strong>ate. The hens were fed in commerciallaying cages with 3 birds per cage and free access to feed and water for 39 wk.Then from 40 to 45 wk, the dietary vitamin level was changed with the localtreatment changed to OVN, NRC with Hy.D changed to NRC, and the other 2treatments unchanged. Hen-day laying rate (HDLR), feed intake (ADFI), eggweight (EW), feed conversion rate (FCR), and hen mortality rate (HMR) weredetermined on the basis of weekly repl<strong>ic</strong>ates. The HDLR for treatments 1, 2,3, and 4 at 39 wk were 70.99, 88.02, 86.32, and 85.27%, respectively, withsignif<strong>ic</strong>ant difference from treatment 1 to the other 3 treatments (P < 0.05), and86.48, 83.95, 81.67, and 81.57% at 45 wk, respectively, and that for treatment 1was signif<strong>ic</strong>antly higher than treatment 4 (P < 0.05). The ADFI for treatments 1,2, 3, and 4 at 39 and 45 wk were 105.8 vs. 120.9 g, 120.3 vs. 118.2 g, 118.5 vs.115.3 g, and 120.1 vs. 115.3 g, respectively. After 1 wk later with the change ofvitamin level, the HDLR, ADFI, EW, and FCR for treatment 1 were improvedand then continued to be improved to the level of OVN, with no signif<strong>ic</strong>antinfluence on HMR. After the NRC with Hy.D was changed to NRC, the HDLRfor treatment 3 was trending to decrease to the level of treatment 4 with NRClevel, with no signif<strong>ic</strong>ant influence on ADFI, EW, FCR, and HMR. The resultsfurther showed that OVN was the best at supporting egg laying.Key Words: vitamin, laying hen, performanceThe objectives of this study were to investigate the effects of different levels ofvitamin E on biochem<strong>ic</strong>al indexes of serum and organization in growing layerducks. Four-week-old Jin-Ding growing ducks (n = 216) with similar weight(0.45 ± 0.01) kg were randomly allotted into 6 treatments with 6 repl<strong>ic</strong>ationsand 6 ducks each in a completely randomized design. The six treatments weresupplemented with 0, 10, 20, 40, or 100 IU/kg, respectively, relative to thebasal diet. The entire trial period was 7 wk. The results showed that vitamin Aincreased T-AOC and GSH-Px activity in serum and liver (P > 0.05), additionof 15, 20 IU/kg of vitamin E in diet signif<strong>ic</strong>antly increased SOD activity inserum compared with other groups. However, MDA in serum had a decreasingtendency (P > 0.05), but content of MDA in liver decreased signif<strong>ic</strong>antly (P 0.05). Immune organ indexes (thymus, bursa, and spleen)had a different degree increase compared with 0 IU/kg of vitamin E group;signif<strong>ic</strong>ant effects on immune organ index (thymus and bursa) were observedin growing ducks fed 15 or 20 IU/kg of supplemental vitamin E (P < 0.05). Theconcentration of Cort had the opposite tendency relative to the vitamin E levels.Concentrations of serum T3 and T4 were higher than no supplemented vitamin E(P > 0.05). It is suggested that the optimal supplement amount of vitamin E wasfrom 15 to 20 IU/kg under cage conditions at the stage of young laying ducks.Note: this research is supported by National Natural Science Foundation ofChina (NSFC, No. 30571345).Key Words: vitamin E, young cage-rearing laying ducks, organism biochem<strong>ic</strong>alindexes81 Zinc requirement of yellow broilers from one to twenty-onedays of age. Zongyong Jiang*, Xiaoyan Liu, Guilian Zhou, Shouqun Jiang,and Xianyong Ma, The Key Laboratory of Animal Nutrition and Feed Science(South China) of Ministry of Agr<strong>ic</strong>ulture, Guangdong Publ<strong>ic</strong> Laboratoryof Animal Breeding and Nutrition, Institute of Animal Science, GuangdongAcademy of Agr<strong>ic</strong>ultural Sciences, Guangzhou, Guangdong, P.R. China.This experiment was conducted to investigate dietary zinc level on growthperformance, antioxidant capacity, immune function, and zinc deposition inyellow broilers of 1 to 21 d of age and to estimate the optimal dietary zinc levelfor yellow broilers fed a cornstarch-corn-soybean meal diet. A total of 1,440one-day-old male broilers were randomly assigned to 6 dietary treatments with 6repl<strong>ic</strong>ates of 40 birds. The treatments received the same basal diet supplementedwith 0, 20, 40, 60, 80, and 120 mg/kg of zinc from zinc sulfate (ZnSO 4.H 2O),respectively. The feeding trial lasted for 21 d. The results showed that 1) addingzinc signif<strong>ic</strong>antly improved the growth of broilers from 1 to 21 d of age (P 0.05). Based on criteria includingADG, AKP activity in serum, and zinc concentration in tibia, the estimatedrequirements of zinc all were 85 mg/kg, but the optimal zinc level estimated byNLIN were 99, 96, and 93 mg/kg, respectively.Key Words: zinc deposition, zinc requirement, yellow broiler25
- Page 1 and 2: Inaugural ASAS-CAAVAsia Pacif ic Ri
- Page 3 and 4: Scientific ProgramTable of Contents
- Page 5 and 6: 1 Advanced needle-free injection te
- Page 7 and 8: 9 Pig personality, meat quality, an
- Page 9 and 10: 17 The contamination and distributi
- Page 11 and 12: 25 Genetic evaluations for measures
- Page 13 and 14: of control and the lowest of SDAP g
- Page 15 and 16: 39 Effects of bacterial protein and
- Page 17 and 18: Advances in Digestive Physiology Me
- Page 19 and 20: L-arginine increased (P < 0.05) the
- Page 21 and 22: average final weight (AFW) and aver
- Page 23: 71 Building a foundation: Cells, st
- Page 27 and 28: 86 Evaluation of phosphorus excreti
- Page 29 and 30: 94 Responses of dairy cows to suppl
- Page 31 and 32: 102 Construction and analysis of a
- Page 33 and 34: M132 Study on the effects of pectin
- Page 35 and 36: M140 Effect of Mintrex Zn on perfor
- Page 37 and 38: M148 Effect of the hydrolyzed wheat
- Page 39 and 40: treatment 1 was significantly lower
- Page 41 and 42: M163 The main fatty acid contents i
- Page 43 and 44: M170 Zinc requirements of yellow br
- Page 45 and 46: M178 Influences of dietary riboflav
- Page 47 and 48: M185 Application of an advanced syn
- Page 49 and 50: M193 Studies on the effects of oreg
- Page 51 and 52: M202 Plasma leucine turnover rate,
- Page 53 and 54: 103 Use of natural antimicrobials t
- Page 55 and 56: 111 The somatotropic axis in growth
- Page 57 and 58: Environmental Impacts of Cattle, Sw
- Page 59 and 60: 128 Opportunities for international
- Page 61 and 62: Animal Health PostersT211 Locoweed
- Page 63 and 64: T219 Stabilization of roxarsone and
- Page 65 and 66: Beef Species PostersUrinary purine
- Page 67 and 68: T233 The effects of sire and breed
- Page 69 and 70: T242 Ultrastructure of oocyte and e
- Page 71 and 72: T249 Effect of different combinatio
- Page 73 and 74: Forages and Pastures PostersIn vitr
- Page 75 and 76:
T263 Effects of leaf meal of Brouss
- Page 77 and 78:
T271 The effects of feeding expandi
- Page 79 and 80:
Lactation Biology PostersT278 Effec
- Page 81 and 82:
Physiology and Endocrinology Poster
- Page 83 and 84:
T288 Effect of Aspergillus meal pre
- Page 85 and 86:
Poultry Physiology, Endocrinology,
- Page 87 and 88:
T301 Observation of the feeding man
- Page 89 and 90:
T307 Effect of levels of Yucca schi
- Page 91:
T313 Study of lysine requirement of
- Page 94 and 95:
energy, 5, 26energy and nutrient di
- Page 96 and 97:
protein digestive enzyme, 44protein
- Page 98 and 99:
HHai, Y., T222, T248Hai-Ying, Z., T
- Page 100 and 101:
Song, X., T223Song, Z. G, M144, T20
- Page 102:
102NOTES