Inaugural ASAS–CAAV Asia Pacif ic Rim Conference Abstracts

M140 Effect of Mintrex Zn on performance and eggshellquality in laying hens. S. Qiujuan, W. Jinlei, Z. Tianguo, and G. Yuming*,College of Animal Science and Technology China Agricultural University,Beijing, China.The study was conducted to assess efficacy of Mintrex Zn (Novus International,Inc., St. Charles, MO, USA) on performance of laying hens in differentsupplemental ways. A total of one-hundred-eight 37-wk-old Hyline brownlayers were randomly allotted to 2 treatments with 6 replicates per treatment(3 pens per replicate with 3 layers each). The control is the basal diet withinorganic minerals (CuSO 4, ZnSO 4and MnSO 4separately, i.e., Cu 10 ppm,Zn 30 ppm and Mn 30 ppm). The second treatment was supplemented withcombination of Mintrex Zn at 20 ppm and sulfite Zn at 10 ppm. The diet wasformulated based on NRC (1994). The trace minerals in basal diets were Cu 6.5ppm, Mn 17 ppm and Zn 20 ppm, respectively, based on the actual analysis. Alldiets were isoenergetic, isonitrogenous and isomethoine, part of the Met sourcewas provided from Mintrex (Mintrex provides about 80% Met activity). Theexperiment lasted for ten weeks from 39- to 48-wk-old. The results showedthat supplementation of Mintex Zn to replace sulfite Zn 20 ppm of 30 ppm hasno significant effects on egg production and egg shell quality in laying hensduring the experimental period. However, egg weight, and egg mass tendedto be increased, and FCR tended to be improved numerically by Mintrex Znsupplementation. Zn levels in tissues (sera, liver, pancreas, spleen and phalanxand yolk) was not significantly affected. Hepatic enzyme activities weresignificantly enhanced (P < 0.05) by Mintrex Zn supplementation, howeverCuZn-SOD and AKP were not affeted. The blood lymphocyte proliferationexposed to ConA or LPS and BSA antibody production on day 10 post primaryand secondary injection of BSA was not influenced by Mintrex Zn. In brief,dietary Mintrex Zn to replace sulfite Zn at 20 ppm for the soybean meal typelayer’s diet improved FCR and egg weights but not egg production; Bothhumoral and cellular immunity, tissue Zn retention and CuZn-SOD and AKPactivities in hepatic tissues was not significantly influenced, however, Ca wassignificantly enhanced by Mintrex Zn.Key Words: Mintrex Zn, layers, performanceM141 Effect of Mintrex Cu on performance and eggshellquality in laying hens. S. Qiujuan, W. Jinlei, Z. Tianguo, and G. Yuming*,College of Animal Science and Technology China Agricultural University,Beijing, China.The study was conducted to assess efficacy of Mintrex Cu (Novus International,Inc., St. Charles, MO, USA) on performance of laying hens in differentsupplemental ways. A total of one-hundred-eight 37-wk-old Hyline brownlayers were randomly allotted to 2 treatments with 6 replicates per treatment(3 pens per replicate with 3 layers each). The control is the basal diet withinorganic minerals (CuSO 4, ZnSO 4and MnSO 4separately, i.e. Cu 10 ppm,Zn 30 ppm and Mn 30 ppm). The second treatment was supplemented withcombination of Mintrex Cu at 20 ppm and sulfite Cu at 10 ppm. The dietwas formulated based on NRC (1994). The trace minerals in basal diets wereCu 6.5 ppm, Mn 17 ppm and Zn 20 ppm respectively based on the actualanalysis. All diets were isoenergetic, isonitrogenous and isomethoine, partof the Met source was provided from Mintrex (Mintrex provides about 80%Met activity). The experiment lasted for ten weeks from 39- to 48-wk-old. Theresults showed Mintex Cu to replace sulfite Cu didn’t influence FI, BW, eggproduction and egg mass of layers. FCR tended to be improved numericallyby Mintrex Cu. The Cu concentration in tissues (sera, and pancreas andphalanx) and the enzyme activity of hepatic CuZn-SOD was not influenced,and Cu level in egg yolk at wk 4 and hepatic and spleen Cu concentrationswas significantly (P < 0.05) increased and ceruloplasmin activity (P = 0.068)at wk 9. Mintrex Cu didn’t influence the blood lymphocyte proliferationexposed to ConA or LPS. The BSA antibody production on d 10 post bothprimary and secondary injection of BSA was influenced by Mintrex Cu.Key Words: Mintrex Cu, layers, perofrmanceM142 Effect of phytase with different dose and dosage-formon the performance and utilization of calcium and phosphorus in layinghens. M. Qiu* 1 , X. Zhang 1 , L. Wang 1 , B. Guo 2 , J. Su 2 , and T. Wang 1 , 1 Collegeof Animal Science and Technology, Nanjing Agricultural University, Nanjing,Jiangsu, P.R. China, 2 Beijing Smile Feed Science and Technology CompanyLimited, Beijing, P.R. China.An experiment was conducted to evaluate the effects of phytase with differentdose and dosage-form on the performance and utilization of calcium (Ca) andphosphorus (P) in laying hens. A total of eight hundred and forty 18-week-oldlaying hens were randomly allocated into 7 groups with 6 replicates of 20 birdseach. Group I, as the positive control, was fed with the basal diet, Group II, asthe negative control, was fed with low-available phosphorus (AP) diet, whilegroup III, IV and V, as trial groups, were fed with low-AP diet adding coatingdosage-form phytase at three levels (300, 1000 and 10000U/kg, respectively),group VI and VII, as the same, were supplemented with 300U/kg phytase withpowdery and granular dosage-form, respectively. The results indicated that,compared with the negative control, the addition of phytase to the low-AP dietas the only variable did not affect egg gravity, broken-egg rate or ADFI (P >0.05), but enhanced the laying production, laying rate (P < 0.05) and reducedthe feed conversion ratio (FCR) significantly (P < 0.01), meanwhile, phytaseaddition did not affect eggshell hardness and thickness(P > 0.05), but both hadbeen improved slightly; Phytase supplementation increased the concentrationof P (P > 0.05) in blood and reduced the activity of serum alkaline phosphatase(AKP) (P < 0.05) significantly; The contents of tibia Ca and P of trial groupshad been increased to a greater extent(especially tibia P) (P > 0.05) and theapparent digestibility of Ca and P of the trial groups were improved (P < 0.05)significantly Compared with the negative control, there were no differencesamong the positive control and the trial groups. It was concluded that theaddition of phytase to low-AP diets did increase the performance and utilizationof Ca and P, and phytase addition with 300U/kg was enough to meet the nutrientrequirement of laying hens for growth and production. Our results appear toconfirm that coating would be a suitable dosage-form of phytase in laying hens,then was the granular, the powdery phytase was inferior to the formers.Key Words: phytase, performance, calcium, phosphorus, utilization, layinghensM143 The comparative analysis on egg quality, nutrientsand relative gene with gene expression profile of embryo by cDNAmicroarray from Lingshan Local breed and White Plymouth Rock breed.Y. Feng, X. Wang*, C. Zhang, P. Zeng, G. Shu, Q. Luo, and D. Zhang, College ofAnimal Science, South China Agricultural University, Guangzhou, GuangdongProvince, China.A experiment was conducted to study the comparative analysis on egg quality,nutrients and relative gene with gene expression profile of embryo by cDNAmicroarray from Lingshan Local breed and White Plymouth Rock breed. Eggswere obtained from two breeds of chickens(Lingshan Local breed and WhitePlymouth Rock) with similar age (33 wk) and received diets formulated tocontain the same dietary energy and protein levels within a farm. Sixty eggswere taken at random from each breed. Thirty eggs from each breed wereused to analyze egg quality, components of fatty acid, and amino acid. Thirtyeggs were used to evaluate the gene expression with gene expression profileby cDNA microarray at 4, 10, 20 embryonic age. All data were made usingindependent-samples T-Test by SAS (SAS Institute, Cary, NC, USA). Theresults showed that: (1) egg and albumen weight of White Plymouth Rock breedwere significantly higher than Lingshan Local breed. Lingshan Local breed hadsignificantly higher yolk weight and yolk/albumen ratio than White PlymouthRock breed. (2) The content of water in yolk and albumen of White PlymouthRock breed were significantly higher than Linshan Local breed. (3) The contentof total amino acids and necessary amino acids of Lingshan Local breed weresignificantly higher than White Plymouth Rock breed. (4) The content of fattyacids except r-linolenic acid and arachidonic acid of Lingshan Local breed werehigher than White Plymouth Rock breed. (5) The genes associated with aminometabolism (Cys, Phe, Try, Tyr, Glu and His) were significantly different in20 embryonic age between the two breeds. These results provided here mayhelp us to understand the mechanism of how maternal nutrition affected thepostnatal growth and be valuable for future metabolic programming studies.This work was supported by The Joint Funds of the National Natural ScienceFoundation of China (U0931004).Key Words: Lingshan Local breed, White Plymouth Rock breed, egg quality35