Inaugural ASASâCAAV Asia Pacif ic Rim Conference Abstracts

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T280 Developmental changes of the milk protein fromcolostrum to milk in the transition dairy cow. S. S. Li, J. Q. Wang*, H. Y.Wei, Y. X. Yang, L. Y. Zhang, C. L. Zhang, and D. P. Bu, State Key Laboratory ofAnimal Nutrition, Institute of Animal Science, Chinese Academy of AgriculturalSciences, Beijing, China.Bovine colostrum, compared to milk, contains bioactive substances that canenhance immune function and promote growth of neonatal calves. The purposeof this study was to analyze the changes of milk protein from colostrum to milk.Milk proteins of dairy cows from the first, seventh, and 21st day after calvingwere separated and identified by 2-dimensional electrophoresis in combinationwith HPLC tandem ion trap spectrometry. The results showed that 6 proteinspots were more abundant in first day samples, compared to the spots of theseventh and 21st day, and that expression of the proteins did not significantlychange between the later samples. The proteins, including the immune functionproteins IgM and IgG and transport proteins transferrin and albumin, wereidentified. The findings may provide valuable information for exploring theimmune function decrease in periparturient dairy cattle.Key Words: 2-dimensional electrophoresis, milk, spectrometryT281 Effect of daidzein and genistein on proliferation andantioxidation of mammary epithelial cell of dairy cow in vitro. C. Liu 1 ,Z. Li 2 , and A. Shan* 3 , 1 Heilongjiang Key Laboratory of Blacksoil Ecology,Northeast Institute of Geography and Agroecology, Chinese Academy ofSciences, Harbin Heilongjiang Province, P.R. China, 2 Animal Science ResearchCentre, Hei Long Jiang Academy of Agricultural Research, Harbin HarbinHeilongjiang Province, P.R. China, 3 Animal Nutrition Institute, NortheastAgricultural University, Harbin Heilongjiang Province, P.R. China.Daidzein and genistein glucuronides, major isoflavone metabolites, may bepartly responsible for biological effects of isoflavones, such as estrogen receptorbinding and natural killer cell activation or inhibition. This study was carriedout to investigate the effect of daidzein and genistein at 1, 10, 100, or 1,000ng/mL on the proliferation and antioxidation of mammary epithelial cells ofdairy cow in vitro. The proliferation experiment results showed that 10 to 100ng/mL of genistein and 100 to 1,000 ng/mL of daidzein improved dairy cowmammary epithelial cell proliferation compared to the blank control group (P
Physiology and Endocrinology PostersT282 The effect of melatonin on chicken immune functionunder different illuminations. S.-Y. Liu*, D.-Y. Chen, and J.-W. Qi, InnerMongolian Agricultural University, Huhhot, Inner Mongolia Region, China.To study the effect of different levels of melatonin on the immune function ofchicken, 3 groups of 4-wk-old chickens were divided among 6-h short lighting,18-h long lighting, and 12-h control groups. Three tests were conducted after 3 wkof control lighting. Contents of CD3+, CD4+, CD8+T cells and Bu-1a+ B cellswere examined in peripheral blood using flow cytometry. Changes in monocytemacrophage phagocytic reaction were examined by a carbon particles clearancetest. Thymus, spleen, and bursa of Fabricius were stained with hematoxylineosin,and a stage micrometer scale was used to measure thymic corpusclediameter, bursa of Fabricius nodule area, and splenic corpuscle diameter. Theresults showed that contents of CD3+, CD4+, CD8+T cells and Bu-1a+ B cellsincreased significantly under shortened lighting, and the level of melatonin wasinhibited during long lighting. Monocytes macrophage function phagocytic indexwas increased under the shortened lighting time. Differences were significant(P < 0.05) or very significant (P < 0.01) in comparison with the control group.Furthermore, with the lighting time lengthened, thymic corpuscle diameter, bursaof Fabricius nodule area, and splenic corpuscle diameter index were decreasedwithin the same group of birds. Differences were significant (P < 0.05) or verysignificant (P < 0.01) compared with the control group. The observation showedthat the diameter and number of thymic corpuscle in the 6-h lighting groupincreased, and the lymphocytes became denser; the bursa of Fabricius nodulearea enlarged, and lymphocytes became denser. The diameter of the spleniccorpuscle increased, the germinal center of splenic corpuscle became obvious,and the periarterial lymphatic sheaths thickened. The above results indicated thatthe level of melatonin significantly affected quantities of T- and B-lymphocytes,monocyte phagocytic capacity, and the tissue structure of lymphoid organs. Thusmelatonin could enhance a chicken’s immune function.Key Words: melatonin, immune function, chickenT283 Anatomical research on the brain of African ostriches.K. Peng*, Y. Feng, G. Zhang, H. Liu, and H. Song, College of Animal Scienceand Veterinary Medicine, Huazhong Agricultural University, Wuhan, P.R.China.The African ostrich is a unique, 2-toed, extant bird species. There are manyreports about bioecology and breeding of ostriches; however, there are fewreports about morphology. And there is no anatomical report about the centralnervous system of African ostriches. This research provides morphological dataof the African ostrich brain for physiology, thremmatology, and clinical veterinarystudy. A neuroanatomical method was used in this study. Six adult Africanostriches, 6 Pekin ducks, 6 gray geese, and 6 Oriental white storks were studied,each consisting of 3 couples of both sexes. The morphological characteristics ofAfrican ostrich brain are described in this report. The average weight, length, andwidth of the total brain is 26.34 g, 59.26 mm, and 42.30 mm, respectively. Thecerebellum appeared to be relatively well-developed, which obviously protrudesdorsally. The posterior superior part of the cerebellum vermis almost forms anangle of 130°. The number of transverse fissures of the cerebellum vermis wasmuch more than that of the domestic fowls. Therefore, the surface area of Africanostrich cerebellum was relatively larger. The formation of the cerebrum was anobtuse triangle. Its surface was smooth, and there were no gyrus and sulcus on it.The gray matter was very thin. There was an arcuated telencephalic vallicule onthe dorsa1 surface, and the sagittal eminence was an ellipse. The olfactory bulbswere quite smal1. The hypophysis was spherical. The total brain only represented0.015% of the whole body weight, and it was 17 times lighter than the brains ofthe domestic fowls. Statistical analysis showed that the brain weight relative tobody weight is significantly lower (P < 0.01) in African ostriches than in 3 otherbird species investigated. The present study suggests that the brain of Africanostrich is underdeveloped.Key Words: African ostrich, brain, anatomyT284 Maternal protein restriction throughout gestationand lactation affects offspring skeletal muscle characteristics in weaningand finishing Meishan pigs. J. Wang*, X. Li, and R. Zhao, Key Laboratory ofAnimal Physiology & Biochemistry, Nanjing Agricultural University, Nanjing,Jiangsu, China.Maternal protein restriction (MPR) affects postnatal health of offspring, includingpredisposition to obesity and diabetes. The aim of this study was to investigatewhether maternal protein restriction throughout gestation and lactation affectsproperties of skeletal muscle of offspring at weaning and finishing stages. Sixteenprimiparous purebred Meishan sows were assigned randomly into control andprotein restriction (PR) groups. The control sows were fed diets containing12 and 14% CP during gestation and lactation, respectively, whereas PR sowswere subjected to 50% of dietary PR restriction. The offspring were killed atweaning (35 d old) and finishing (8 mo old) stages. The longissimus dorsi andpsoas major muscles were sampled to determine the morphological features,meat quality traits, and myosin heavy chain (MyHC) type composition. The MPRsignificantly reduced body weight and longissimus dorsi and psoas major muscleweight at weaning with decreased myofiber cross-sectional area (CSA). TheMPR significantly down-regulated MyHC IIb mRNA in the longissimus dorsiand MyHC IIx mRNA in the psoas major. After being weaned, pigs in the PRgroup demonstrated growth compensation. The differences in body and muscleweights at weaning disappeared at finishing stage. No significant differenceswere detected for meat quality traits, including pH 24 h, shear force, water-holdingcapacity, cooking loss, drip loss, or meat color, regardless of muscle type.However, a reversed change in MyHC mRNA composition was detected. TheMPR significantly increased CSA, associated with augmented MyHC IIb mRNAin longissimus dorsi, and MyHC IIb and IIx mRNA in psoas major at finishingstage. These results suggest that despite a complete compensation in body weightand muscle weight, MPR causes long-term alteration in MyHC type compositionof skeletal muscles in the pig. It merits further investigation whether such changesmay contribute to metabolic dysfunction and insulin resistance later in life.Key Words: maternal protein restriction, porcine skeletal muscle, myosin heavychainT285 Effect of in ovo leptin administration on posthatchmuscle growth, myofiber characteristics and gene expression in thechicken. P. Liu*, Y. Hu, and R. Zhao, Key Laboratory of Animal Physiology &Biochemistry, Nanjing Agricultural University, Nanjing, Jiangsu, China.A previous study indicated that leptin deposited in the egg may serve as a maternalsignal for programming of offspring growth. To investigate the effect of maternalleptin on muscle growth, we injected 0 μg (control), 0.5 μg (low dose of leptin,LL), or 5.0 μg (high dose of leptin, HL) of recombinant mice leptin in 100 μL ofPBS, respectively, into albumen of SanHuang breeder eggs prior to incubation.The newly hatched chicks were raised under the same conditions until 21 d ofage when samples of serum, liver, and gastrocnemius muscle were collected foranalyses. Myosin ATPase staining was applied to identify myofiber types and tomeasure cross-sectional area (CSA) and myofiber density (MFD) of myofibers.Real-time PCR was performed to quantitate IGF-1, myostatin (MSTN), andleptin receptor (LEPR) mRNA expression. Western blot analysis was used todetermine hepatic leptin and muscle LEPR protein contents. A RIA was used todetect serum leptin concentration. Male chickens treated with high and low dosesof leptin demonstrated significantly greater (P < 0.05) body weights than thosein the control group. A high dose of leptin significantly augmented CSA (P
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Inaugural ASAS-CAAVAsia Pacif ic Ri
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Scientific ProgramTable of Contents
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1 Advanced needle-free injection te
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9 Pig personality, meat quality, an
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17 The contamination and distributi
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25 Genetic evaluations for measures
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of control and the lowest of SDAP g
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39 Effects of bacterial protein and
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Advances in Digestive Physiology Me
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L-arginine increased (P < 0.05) the
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average final weight (AFW) and aver
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71 Building a foundation: Cells, st
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78 Effect of the level of vitamin A
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86 Evaluation of phosphorus excreti
Page 29 and 30: 94 Responses of dairy cows to suppl
Page 31 and 32: 102 Construction and analysis of a
Page 33 and 34: M132 Study on the effects of pectin
Page 35 and 36: M140 Effect of Mintrex Zn on perfor
Page 37 and 38: M148 Effect of the hydrolyzed wheat
Page 39 and 40: treatment 1 was significantly lower
Page 41 and 42: M163 The main fatty acid contents i
Page 43 and 44: M170 Zinc requirements of yellow br
Page 45 and 46: M178 Influences of dietary riboflav
Page 47 and 48: M185 Application of an advanced syn
Page 49 and 50: M193 Studies on the effects of oreg
Page 51 and 52: M202 Plasma leucine turnover rate,
Page 53 and 54: 103 Use of natural antimicrobials t
Page 55 and 56: 111 The somatotropic axis in growth
Page 57 and 58: Environmental Impacts of Cattle, Sw
Page 59 and 60: 128 Opportunities for international
Page 61 and 62: Animal Health PostersT211 Locoweed
Page 63 and 64: T219 Stabilization of roxarsone and
Page 65 and 66: Beef Species PostersUrinary purine
Page 67 and 68: T233 The effects of sire and breed
Page 69 and 70: T242 Ultrastructure of oocyte and e
Page 71 and 72: T249 Effect of different combinatio
Page 73 and 74: Forages and Pastures PostersIn vitr
Page 75 and 76: T263 Effects of leaf meal of Brouss
Page 77 and 78: T271 The effects of feeding expandi
Page 79: Lactation Biology PostersT278 Effec
Page 83 and 84: T288 Effect of Aspergillus meal pre
Page 85 and 86: Poultry Physiology, Endocrinology,
Page 87 and 88: T301 Observation of the feeding man
Page 89 and 90: T307 Effect of levels of Yucca schi
Page 91: T313 Study of lysine requirement of
Page 94 and 95: energy, 5, 26energy and nutrient di
Page 96 and 97: protein digestive enzyme, 44protein
Page 98 and 99: HHai, Y., T222, T248Hai-Ying, Z., T
Page 100 and 101: Song, X., T223Song, Z. G, M144, T20
Page 102: 102NOTES
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Inaugural ASASâCAAV Asia Pacif ic Rim Conference Abstracts