Inaugural ASASâCAAV Asia Pacif ic Rim Conference Abstracts

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Growth and Development. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 76International Animal Agriculture . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 77Lactation Biology . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 79Physiology and Endocrinology . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 81Poultry Environment and Management. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 83Poultry Genetics. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 84Poultry Immunology . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 84Poultry Physiology, Endocrinology, and Reproduction. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 85Production, Management and the Environment . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 86Small Ruminants . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 88Swine Species . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 91Subject Index . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 93Author Index . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 974
1 Advanced needle-free injection technology. W. Shao* 1 ,C. Funk 2 , and J. Poiron 2 , 1 Sino Waypoint Consulting, Inc., Ottawa, Ontario,Canada, 2 AcuShot, Inc., Winnipeg, Manitoba, Canada.AcuShot patented technology is the next generation in needle-free liquidinjection devices specifically designed for agricultural use. This technologyprovides the veterinarian and animal health care industries with revolutionaryneedle-free device choices that enable spot treatment of single animals andmass vaccination of large herds of animals in a cost-effective, efficient, safe,and easy-to-use format. AcuShot technology has been adopted in the AcuShotneedle-free injection device for delivering liquid medication under pressureinto an animal. The AcuShot needle-free injection device is a battery-operated,high-workload, handheld, mass-vaccination injector. It can be used on ahands-free stand for smaller livestock, such as newborn piglets and poultry,or can be used with a remote injection handpiece for easy use with largerlivestock, such as the most seasoned sow or cow. The injector can administerany vaccine or medication intramuscularly, subcutaneously, or intradermally(transdermally). AcuShot needle-free injection devices come in two models:1) The AcuShot S provides extremely accurate doses at micro levels and candeliver traditional vaccines or supplements in doses ranging from 0.05 mL upto 1.35 mL in increments of 0.05 mL; and 2) the AcuShot A provides extremelyaccurate doses at levels ranging from 0.2 to 2.5 mL in increments of 0.1 mL.Both the AcuShot S and AcuShot A are electronically controlled and haveonboard electronics that monitor key components and injection characteristicsfor each shot and that provide accurate dosage selection and delivery. AcuShotinjectors are easily adapted to any supply container, regardless of size. TheAcuShot needle-free injection device is able to perform thousands of injectionsper battery charge, eliminating downtime and increasing efficiency. It is saferfor livestock, with a reduced chance of infection or disease transmission, andeliminates the ongoing cost and disposal of hazardous needles. It has been usedin Canada, the United States, Mexico, South America, Japan, Korea, and manycountries in the European Union. It has recently been introduced in China.Sunday, November 8, 2009SYMPOSIA AND ORAL SESSIONSAnimal Health, Growth, Physiology, Endocrinology3 Lipoic acid attenuates the anaphylactic reactions inducedby soybean β-conglycinin in a rat model. P. F. Han*, X. Ma, and J. D. Yin,State Key Laboratory of Animal Nutrition, College of Animal Science andTechnology, China Agricultural University, Beijing, China.The purpose of this study was to evaluate the effects of feeding a low dose oflipoic acid on attenuating soybean β-conglycinin-induced hypersensitivity byusing a rat model, with ovalbumin as the positive allergic control. Forty-eightrecently weaned, male, Sprague-Dawley rats were assigned to 1 of 4 treatmentsand fed a cornstarch- and casein-based diet either unsupplemented (groups I,II, and III) or supplemented with 25 mg/kg of lipoic acid (group IV). Rats ingroups III and IV were sensitized with 20 mg of β-conglycinin on d 1, 10, 17,and 24 by means of intragastric gavage, whereas rats in group II were sensitizedwith 20 mg of ovalbumin. The control group (group I) was gavaged with caseinby using the same treatment schedule. On d 31, rats received a double dose ofβ-conglycinin, ovalbumin, or casein, respectively. Blood was obtained from thetail vein of each rat 3 h after intragastric gavage. On d 32, all rats were slaughteredby cervical dislocation. The spleen and small intestine were removed, and thenthe intestinal tissues were stored in liquid nitrogen until analysis. Untreated,β-conglycinin-sensitized rats (group III) demonstrated an increase in serum IgEand histamine release, but had reduced growth performance and poorer feedconversion compared with the control rats (P < 0.05; group I), similar to theovalbumin-sensitized rats (group II). A low dose of lipoic acid significantly(P < 0.05) improved BW gain and feed conversion while reducing serumIgE and histamine release. Moreover, our research indicated that lipoic acidsupplementation increased interferon-γ but decreased IL-4 (P < 0.05), whichmeans that the Th1-type immune response was increased to prevent soybeanallergies. Taken together, a low dose of lipoic acid has the potential to be usedas an immunomodulator to prevent soybean β-conglycinin-induced allergies byamending the balance of cytokines.Key Words: α-lipoic acid, β-conglycinin, anaphylaxisKey Words: needle free, injection, vaccination2 Isolation of mink enteritis virus and application of immuneyolk antibody. T. Tingting* and Z. Yanlong, Northeast Forest University,Harbin, China.Mink enteritis virus (MEV) infection is a highly contagious disease with amortality of up to 90%. Since this disease was first recognized in Canada in1949 by Schofield, it has been prevalent all over the world, mainly because thevirus can survive in harsh environmental conditions for a long time and evolveby natural mutation. Mink enteritis is characterized by severe mucoenteritis.This paper deals with a case of MEV isolated in Jilin Province in 2007; ourpurpose was examine whether the immunoglobulin derived from chicken eggyolk (IgY) against infection by MEV could have any protective effect in mink.Mink enteritis virus samples were collected from infected mink intestines basedon clinical symptoms. The embryonic feline kidney cell line F81 was used toenrich the viruses. The viruses were then identified by plaque assay and culturedin a 96-well plate. Finally, we isolated the virus, which could agglutinate redblood cells of the pig, but not the human, chicken, or rabbit. This was confirmedby PCR amplification by a special primer of MEV. Moreover, the isolated viruscould infect mink. To prepare IgY samples, we vaccinated 14-wk-old layinghens with MEV samples and then extracted egg yolk antibodies with chloroformand DEAE. The hemagglutination-inhibition test, the agar spread method, andindirect ELISA were used to identify the IgY. The anti-MEV IgY were thenused to treat 2- to 3-mo-old mink that were challenged with the isolated MEV.The present research indicated that the cells showed a cytopathogenic effect.The diluter of hemagglutination test range was 26. The gene of the virus wasamplified by PCR reaction. Twenty mink displayed symptoms within 2.5 to4 d after being inoculated with MEV. The titer of IgY was tested as 27 byhemagglutination-inhibition test, was tested as 1:32 by the agar spread method,and was efficient 2 mo after the last immunization. The clinical analysis showedthat 16 mink in the IgY group were cured, 4 in the control group died, and theprotection rate reached 100%.Key Words: isolation, IgY, MEV4 Discrepancies between in vitro and in vivo aflatoxin binding.J. N. Broomhead* and F. Chi, Amlan International, Chicago, IL, USA.An in vitro binding study and an in vivo chicken study were conducted totest the efficacy and safety of organically modified clays (OMC) in bindingaflatoxin B 1(AFL). In vitro mycotoxin binding was conducted at physiologicalconditions of the stomach (pH 3.0), followed by physiological conditions theintestine (pH 6.5) at 50:1 binder-to-toxin ratio. The chick study consisted of250 one-day-old male broiler chicks assigned to 10 treatments, with 5 replicatepens of 5 chicks each. Four OMC were fed either alone (0.5% dietary inclusion)or in combination with 2 ppm of AFL. Chicks were placed in battery broodersand fed the experimental diets for 21 d. On d 21, three birds per replicate wereeuthanized with CO 2and weighed, and blood was drawn from 2 birds per penfor serum chemistry analysis; livers were removed from 3 birds per pen andweighed for determination of relative liver weight (RLW). In vitro AFL bindingresults were highly variable: OMC A, B, C, and D bound 44, 49, 63, and 81%AFL, respectively. Feeding AFL alone reduced (P < 0.05) BW gain (BWG),feed intake, and serum protein and increased RLW (P < 0.05). Contrary to the invitro results, OMC A and B improved (P < 0.05) BWG and OMC A improved (P< 0.05) RLW when added to the 2 ppm of AFL diet. No improvement in serumprotein (P < 0.05) was observed with the inclusion of any OMC to the AFL diet.A significant reduction (P < 0.05) in BWG was observed when feeding OMC Calone, and an increase (P < 0.05) in serum aspartate aminotransferase was seenwhen feeding OMC B or C alone, suggesting possible toxicity when feedingthese OMC. In conclusion, the in vitro AFL binding procedure used may notbe a good predictor of in vivo efficacy, and in vivo studies should always beconducted to validate in vitro results.Key Words: aflatoxin, in vivo, in vitro5
Page 1 and 2: Inaugural ASAS-CAAVAsia Pacif ic Ri
Page 3: Scientific ProgramTable of Contents
Page 7 and 8: 9 Pig personality, meat quality, an
Page 9 and 10: 17 The contamination and distributi
Page 11 and 12: 25 Genetic evaluations for measures
Page 13 and 14: of control and the lowest of SDAP g
Page 15 and 16: 39 Effects of bacterial protein and
Page 17 and 18: Advances in Digestive Physiology Me
Page 19 and 20: L-arginine increased (P < 0.05) the
Page 21 and 22: average final weight (AFW) and aver
Page 23 and 24: 71 Building a foundation: Cells, st
Page 25 and 26: 78 Effect of the level of vitamin A
Page 27 and 28: 86 Evaluation of phosphorus excreti
Page 29 and 30: 94 Responses of dairy cows to suppl
Page 31 and 32: 102 Construction and analysis of a
Page 33 and 34: M132 Study on the effects of pectin
Page 35 and 36: M140 Effect of Mintrex Zn on perfor
Page 37 and 38: M148 Effect of the hydrolyzed wheat
Page 39 and 40: treatment 1 was significantly lower
Page 41 and 42: M163 The main fatty acid contents i
Page 43 and 44: M170 Zinc requirements of yellow br
Page 45 and 46: M178 Influences of dietary riboflav
Page 47 and 48: M185 Application of an advanced syn
Page 49 and 50: M193 Studies on the effects of oreg
Page 51 and 52: M202 Plasma leucine turnover rate,
Page 53 and 54: 103 Use of natural antimicrobials t
Page 55 and 56:
111 The somatotropic axis in growth
Page 57 and 58:
Environmental Impacts of Cattle, Sw
Page 59 and 60:
128 Opportunities for international
Page 61 and 62:
Animal Health PostersT211 Locoweed
Page 63 and 64:
T219 Stabilization of roxarsone and
Page 65 and 66:
Beef Species PostersUrinary purine
Page 67 and 68:
T233 The effects of sire and breed
Page 69 and 70:
T242 Ultrastructure of oocyte and e
Page 71 and 72:
T249 Effect of different combinatio
Page 73 and 74:
Forages and Pastures PostersIn vitr
Page 75 and 76:
T263 Effects of leaf meal of Brouss
Page 77 and 78:
T271 The effects of feeding expandi
Page 79 and 80:
Lactation Biology PostersT278 Effec
Page 81 and 82:
Physiology and Endocrinology Poster
Page 83 and 84:
T288 Effect of Aspergillus meal pre
Page 85 and 86:
Poultry Physiology, Endocrinology,
Page 87 and 88:
T301 Observation of the feeding man
Page 89 and 90:
T307 Effect of levels of Yucca schi
Page 91:
T313 Study of lysine requirement of
Page 94 and 95:
energy, 5, 26energy and nutrient di
Page 96 and 97:
protein digestive enzyme, 44protein
Page 98 and 99:
HHai, Y., T222, T248Hai-Ying, Z., T
Page 100 and 101:
Song, X., T223Song, Z. G, M144, T20
Page 102:
102NOTES
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Inaugural ASASâCAAV Asia Pacif ic Rim Conference Abstracts