42 Eff<strong>ic</strong>iency of methods applied for goat estrous synchronizationin subtrop<strong>ic</strong>al climate zone. Y. Zhao* 1 , J. Zhang 1 , H. Wei 2 , X. Sun 3 , M. Yu 1 ,L. Wang 1 , B. Mu 1 , and C. Zhao 1 , 1 College of Animal Science and Technology,Chongqing Key Laboratory of Forage and Herbivore, Southwest University,Beibei, Chongqing, China, 2 Faculty of Laboratory Animals, Third MilitaryMed<strong>ic</strong>ine University, Shapingba, Chongqing, China, 3 Department of Anatomy,Med<strong>ic</strong>al College, Jinggangshan University, Jinggangshan, Jiangxi, China.The aim of this study was to select one eff<strong>ic</strong>ient procedure used for estroussynchronization in indigenous Chinese goats and then to evaluate its potentialwhen applied in goat production in a subtrop<strong>ic</strong>al climate zone. In a randomizedblock design, a total of 80 indigenous does were divided into 4 groups and thedoes in group A were treated estrous synchronization by intravaginal spongesimpregnated with 30 mg of levonorgestrel inserted for 10 d. Does in groups B, C,and D were treated with a further injection of 25 IU of FSH, 0.05 mg of PGF 2α,and 25 IU of FSH + 0.05 mg of PGF 2αat sponge withdrawal, respectively.The eff<strong>ic</strong>iency of estrus synchronization and reproductive performancewas evaluated. A total of 140 does on 5 goat farms in the breeding and thenonbreeding season in different counties of southwestern China were treatedwith the selective procedure. Results revealed that all the treatments used werecapable of inducing and synchronizing estrus in goats. The use of intravaginalsponges impregnated with 30 mg of levonorgestrel and 0.05 mg PGF 2αis thefirst cho<strong>ic</strong>e for estrous synchronization in indigenous goats in southwesternChina. Ninety-five percent of treated does in group C demonstrated estrus. Thiswas signif<strong>ic</strong>antly higher than in group A (P < 0.05). There was a signif<strong>ic</strong>antdelay in the onset of estrus (51.79 ± 6.99 h). The time to onset of estrus in thetreatment with FSH (groups B and D) was signif<strong>ic</strong>antly shorter (P < 0.01). Thepercentage of goats ovulating and the ovulation rates among different groupswere not signif<strong>ic</strong>ant (P > 0.05). When the selective procedure was applied on5 goat farms, 85.71% (120/140) of the does demonstrated estrus. The averagekidding percentage and litter size were 62.50% and 1.77, respectively. The doesin Dujiangyan, wh<strong>ic</strong>h were group-housed and had estrus induced in the breedingseason, showed the greatest estrous response (94.29%), kidding percentage(81.82%), and litter size (1.96 ± 0.44). Results ind<strong>ic</strong>ated that the treatment usedwas capable of inducing and synchronizing estrus in goats in this area.Key Words: goat, estrous synchronization, nonbreeding seasonSYMPOSIA AND ORAL SESSIONSSmall Ruminant, Forages and Pasturesmean was lower for KK kids (789 eggs/g) than for BK and KB kids (1,511,1,557 eggs/g). In conclusion, breed can signif<strong>ic</strong>antly affect the postweaningperformance of meat goat kids on pasture.Key Words: meat goat, breed, growth44 Effects of Chinese herbal med<strong>ic</strong>ine compoundsupplementation to the basal diet on the main protein digestive enzymes ingrowing beef cattle. H. F. Wang 1 , W. R. Yang* 1 , Z. B. Yang 1 , Y. H. Cui 1 , andY. Wang 2 , 1 Shandong Agr<strong>ic</strong>ultural University, Tai-an, Shandong, P. R. China,2Agr<strong>ic</strong>ulture and Agri-Food Canada, Lethbridge Research Centre, Lethbridge,Canada.An experiment was conducted to evaluate the effects of 3 kinds of Chineseherbal med<strong>ic</strong>ine compounds (CHMC) on the activity of the main proteindigestive enzymes in growing beef cattle. A total of 32 crossbred beef cattle(Limousin × Lu-xi, 199.37 ± 8.67 kg) were randomly divided into 4 treatments.The control group was fed a basal diet (no herbs added) and 3 tested groupswere fed the basal diet supplemented with 3 kinds of CHMC, respectively,A, B, and C. The addition level of herbals was 2% of concentrate feed. Thewhole experiment was divided into 2 stages: a preliminary test period (10 d)and a test period (90 d). All the data were analyzed using the GLM procedure ofSAS. Adding CHMC improved the activity of pepsin signif<strong>ic</strong>antly (P < 0.05).There was no signif<strong>ic</strong>ant variation in activity of trypsin in the duodenum intest groups (P > 0.05). However, CHMC improved the activity of trypsin inthe jejunum and ileum signif<strong>ic</strong>antly compared with the control group. In thejejunum, prescriptions A and C played the better role than prescription B (P< 0.05). In the ileum, none of the variation was statist<strong>ic</strong>ally signif<strong>ic</strong>ant amongthe 3 prescriptions (P > 0.05). Chymotrypsin activity was slightly higher in theduodenum and ileum in cattle fed CHMC; however, none of the increases werestatist<strong>ic</strong>ally signif<strong>ic</strong>ant (P > 0.05). Supplemental CHMC improved the activityof chymotrypsin in the jejunum signif<strong>ic</strong>antly (P < 0.05). There was no obviousdifference in the activity of chymotrypsin in the jejunum among the differentprescriptions (P > 0.05). It was concluded that CHMC can obviously improvethe activities of pepsin, the activity of trypsin in the jejunum and ileum, and theactivity of chymotrypsin in the jejunum.1643 Sire and dam breed effects on postweaning performance ofpasture-raised meat goat kids. R. Browning Jr.* 1 and M. L. Leite-Browning 2 ,1Tennessee State University, Nashville, TN, USA, 2 Alabama A&M University,Huntsville, AL, USA.Straightbred and reciprocal F 1kids (n = 449) from a complete 3-breed diallel ofBoer (B), Kiko (K), and Spanish (S) were managed from 3 to 6 mo of age across3 yr to assess breed effects on postweaning performance. Kids were weanedin June or August each year and raised on summer pasture with 0.34 kg/d of16% CP supplement. Buck kids remained intact. Growth rate was affected (P= 0.02) by dam breed but not by sire breed (P = 0.99). Kids from Boer damshad lower ADG (52.7 g/d) than those from Kiko and Spanish dams (60.9, 61.4± 3.2 g/d). Weaning class (P = 0.03), kid sex (P < 0.01), and month (P = 0.02)influenced postweaning ADG. Singles has lower ADG than twins (55 vs. 60.9± 2.1 g/d; triplets were intermediate), bucklings had higher ADG than doelings(65.5 vs. 51.4 ± 2.7 g/d), and ADG was higher for June kids than for Augustkids (60.9 vs. 55.5 ± 2.7 g/d). Adjusted 180-d weights were affected by sirebreed (P = 0.05) and dam breed (P < 0.01). Boer- and Kiko-sired kids wereheavier (22.6, 22.6) than Spanish-sired kids (21.6 ± 0.4 kg) and each dam breeddiffered for kid weight (Kiko = 24.1; Spanish = 22.4; Boer = 20.4 kg). Beforecorrection, each wean class differed (P < 0.01) for 180-d kid weight (singles =22.7; twins = 20.2; triplets = 16.9 kg). Kid sex and month each influenced (P
Advances in Digestive Physiology Metabolism and Health46 Maternal and offspring intestinal responses to gestationalnutrition in the ruminant: Growth, vascularity, and angiogen<strong>ic</strong> factorexpression. A. M. Meyer* 1 , D. A. Redmer 1 , J. M. Wallace 2 , K. A. Vonnahme 1 ,L. P. Reynolds 1 , B. W. Hess 3 , and J. S. Caton 1 , 1 Center for Nutrition andPregnancy, Department of Animal Sciences, North Dakota State University,Fargo, ND, USA, 2 Rowett Institute of Nutrition and Health, University ofAberdeen, Bucksburn, Aberdeen, UK, 3 Department of Animal Sciences,Laramie, WY, USA.Small intestinal growth, development, and vascularization are often-overlookedbut essential processes driving nutrient uptake and expenditure, immunolog<strong>ic</strong>alcompetence, neonatal survival, postnatal growth, and metabol<strong>ic</strong> regulationvia a cadre of hormones and growth factors. Tissue vascularization is crucialfor nutrient transport both to and from the intestine; thus, angiogenesis, or theformation of blood vessels, is crit<strong>ic</strong>al for proper intestinal function. Maternalintestinal mass, cellularity, proliferation, and vascularity are responsive tophysiolog<strong>ic</strong>al states such as pregnancy and lactation. Additionally, intestinaldevelopment during the perinatal period includes growth via cell proliferation,hypertrophy, and increased vascularization. Restr<strong>ic</strong>ted and excessive nutrientintake during gestation effect maternal intestinal mass, proliferation, cell size,and vascularity, although these responses are affected by diet composition,stage of gestation, age, and species of the dam. Changes in intestinal mass andblood flow alter both the nutrient needs and nutrient uptake potential by thedam, suggesting possible mechanisms by wh<strong>ic</strong>h the maternal system meets thedemands of gestation and lactation while also partially explaining differencesobserved in fetal development and postnatal performance. Fetal intestinal growthand vascularity have been decreased or increased by altered maternal nutritionin various studies. In some cases the effects of maternal gestational nutritionon the offspring small intestine appear to persist even when offspring receivesimilar postnatal management. Nutritional effects on intestinal vascularityhave been accompanied by changes in messenger RNA expression of vascularendothelial growth factor (VEGF), its receptors, endothelial nitr<strong>ic</strong> oxide (NO)synthesis, and soluble guanylate cyclase (NO receptor), implying possibleregulatory roles of VEGF and NO in these responses. Nutritional modulationor other therapeut<strong>ic</strong>s may provide means to stimulate intestinal blood flow orangiogenesis or both, affording potential opportunity to overcome growth orhealth challenges in ruminants.Key Words: angiogenesis, intestine, ruminant47 Appl<strong>ic</strong>ation of new advances in digestive physiology as theyapply to swine nutrition. J. S. Radcliffe*, Purdue University, West Lafayette,IN.Advances in our knowledge of nutrition and digestive physiology are beingaided by an increasing array of molecular biology techniques. This allowsfor a fundamental understanding of various processes that may impact ourunderstanding of how pigs should be fed. However, at times there is a largedisconnect between bas<strong>ic</strong> research findings and their appl<strong>ic</strong>ation on the farm.This talk will focus on recent, bas<strong>ic</strong> research findings and their potentialappl<strong>ic</strong>ation to swine nutrition. In part<strong>ic</strong>ular the talk will focus on recentdevelopments in our understanding of nutrient absorption and regulation, andhow this information may be used in the future to more accurately feed swine.Key Words: digestive physiology, nutrition48 In vivo and in vitro immunomodulation by mannanoligosaccharides in pigs. T. M. Che* and J. E. Pettigrew, Department ofAnimal Sciences, University of Illinois, Urbana-Champaign, IL, USA.Mannan oligosaccharides (MOS), derived from the cell wall of yeastSaccharomyces cerevisiae, has been shown to affect innate and humoralimmunity in animals. The MOS enhances phagocytosis by murine phagocytesand reduces the intensity of the wattle hypersensitivity reaction in pullets.Feeding MOS to animals increases the immunoglobulin levels in plasma, bile,and colostrum. These findings suggest that MOS potentially have the abilityto affect cytokine secretion by stimulated macrophages and to modulate theimmune response of animals against surrounding immunolog<strong>ic</strong>al challenges.Recent discovery reveals that MOS may enhance the immune response viaactivation of cytokine release but prevent overstimulation of the pig’s immunesystem. The MOS can activate porcine alveolar macrophages to secrete tumornecrosis factor-α (TNF-α). However, MOS, when fed to pigs or appliedin vitro, alleviates the inflammatory response of alveolar macrophages byreducing TNF-α and increasing IL-10. Further, immunomodulatory functionsof MOS have been proved in a porcine reproductive and respiratory syndromevirus (PRRSV) challenge study. As we know, PRRSV strongly modulates thehost's immune responses through reducing the total and differential leukocytecounts, inducing a variety of cytokines and delaying cell-mediated immunity.Dietary MOS is associated with rapidly increased numbers of total white bloodcells, lymphocytes, and neutrophils in PRRSV-infected pigs at the early stage ofinfection. Infected pigs fed MOS have a lowered fever, reduced serum TNF-α,and increased serum IL-10. Additionally, the data analysis from the AffymetrixGeneChip Porcine Genome Array ind<strong>ic</strong>ates that MOS changes the expressionof thousands of genes caused by PRRSV in peripheral blood mononuclear cellsand bronchoalveolar lavage fluid cells. Briefly, modulation of the immunestatus of pigs via dietary MOS may promisingly bring favorable effects andprovide a novel prospect in improving pig health and production, but furtherresearch is still needed.Key Words: immunomodulation, mannan oligosaccharides, pigs49 A comparative study of three diagnost<strong>ic</strong> techniques(FIRSTtest, polymerase chain reaction, and enzyme-linked immunosorbentassay) for detecting porcine ileitis on a pig farm. D. J. Yu 2 , C. K. Mah* 1 ,X. H. Li 1 , and B. Yin 2 , 1 Elanco Animal Health, Shanghai, China, 2 College ofAnimal Science, Fujian Agr<strong>ic</strong>ulture and Forestry University, Fuzhou, Fujian,China.The main objective of this cross-sectional study was to compare and evaluatethree different diagnost<strong>ic</strong> techniques, namely FIRSTtest, PCR, and ELISA,used in detecting ileitis infection status in a pig farm. A total of 120 pigs with15 pigs in each different age group (3, 6, 9, 12, 15, 18, 21, and 24 wk of age)were randomly tested. The results showed that tool pigs from 9 to 12 wk of agehad a higher positive rate of ileitis tested with FIRSTtest and PCR diagnost<strong>ic</strong>techniques. In this study, FIRSTtest was found to be more sensitive thanPCR in detecting Lawsonia intracellularis antigen in porcine fecal samples.The positive samples tested with FIRSTtest and PCR showed a signif<strong>ic</strong>antcorrelation with the soft tool samples from the ileitis-suspected pigs. FIRSTtesttechnique was also found to be valid because it had good correlation with theserology findings. Trends of positive rate for porcine ileitis tested with ELISAmethod lagged behind those tested by FIRSTtest and PCR by as much as 4wk. The study findings ind<strong>ic</strong>ated that FIRSTtest, being a recently developedqualitative test, is a qu<strong>ic</strong>k, reliable, and easy-to-use test kit to detect porcineileitis on the farm site.Key Words: porcine ileitis, FIRSTtest, comparative study50 Pig personality, obesity and metabol<strong>ic</strong> programming. R.Zhao*, X. Yang, D. Xia, L. Li, S. Wei, and Q. Sun, Nanjing Agr<strong>ic</strong>ulturalUniversity, Nanjing, China.Different breed of pigs differ in personality, growth rate and carcass quality,yetthe mechanism underlying the formation and integration of the breed-specif<strong>ic</strong>traits is elusive. In the present study, Chinese Erhualian (EHL) and EuropeanPietrain (PIE) pigs were subjected to a coping characterist<strong>ic</strong> test, Backtest, at3, 10 and 17 days of age and a 2 hrs transport trial at the body weight of 20kg. We observed distinct breed differences not only in growth and obesity, butalso in stress-coping styles. EHL pigs demonstrated higher stress resistancecompared to PIE pigs, wh<strong>ic</strong>h was associated with 2-fold higher basal plasmacortisol concentration yet lower cortisol increase responding to stress. Higheradrenocort<strong>ic</strong>al steroidogen<strong>ic</strong> capacity, characterised by enhanced ACTHsignaling and augmented expression of StAR and steroidogen<strong>ic</strong> enzymes, mayresult in higher basal cortisol level, whereas higher expression of GR, MR,11βHSD1, but lower 11β-HSD2 in hippocampus may account for higher stresstolerance in EHL pigs owing to a more effective negative feedback regulationof HPA axis activity. GR expression was found to be breed-specif<strong>ic</strong> in otherperipheral tissues including liver, muscle and fat, wh<strong>ic</strong>h is associated withbreed-specif<strong>ic</strong> properties in hepat<strong>ic</strong> gluconeogenesis, muscle energy metabolisnand fat deposition. To test the hypothesis that the breed-specif<strong>ic</strong> phenotypes andpattern of GR expression in EHL pigs is a result of natural selection under the17
- Page 1 and 2: Inaugural ASAS-CAAVAsia Pacif ic Ri
- Page 3 and 4: Scientific ProgramTable of Contents
- Page 5 and 6: 1 Advanced needle-free injection te
- Page 7 and 8: 9 Pig personality, meat quality, an
- Page 9 and 10: 17 The contamination and distributi
- Page 11 and 12: 25 Genetic evaluations for measures
- Page 13 and 14: of control and the lowest of SDAP g
- Page 15: 39 Effects of bacterial protein and
- Page 19 and 20: L-arginine increased (P < 0.05) the
- Page 21 and 22: average final weight (AFW) and aver
- Page 23 and 24: 71 Building a foundation: Cells, st
- Page 25 and 26: 78 Effect of the level of vitamin A
- Page 27 and 28: 86 Evaluation of phosphorus excreti
- Page 29 and 30: 94 Responses of dairy cows to suppl
- Page 31 and 32: 102 Construction and analysis of a
- Page 33 and 34: M132 Study on the effects of pectin
- Page 35 and 36: M140 Effect of Mintrex Zn on perfor
- Page 37 and 38: M148 Effect of the hydrolyzed wheat
- Page 39 and 40: treatment 1 was significantly lower
- Page 41 and 42: M163 The main fatty acid contents i
- Page 43 and 44: M170 Zinc requirements of yellow br
- Page 45 and 46: M178 Influences of dietary riboflav
- Page 47 and 48: M185 Application of an advanced syn
- Page 49 and 50: M193 Studies on the effects of oreg
- Page 51 and 52: M202 Plasma leucine turnover rate,
- Page 53 and 54: 103 Use of natural antimicrobials t
- Page 55 and 56: 111 The somatotropic axis in growth
- Page 57 and 58: Environmental Impacts of Cattle, Sw
- Page 59 and 60: 128 Opportunities for international
- Page 61 and 62: Animal Health PostersT211 Locoweed
- Page 63 and 64: T219 Stabilization of roxarsone and
- Page 65 and 66: Beef Species PostersUrinary purine
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T233 The effects of sire and breed
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T242 Ultrastructure of oocyte and e
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T249 Effect of different combinatio
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Forages and Pastures PostersIn vitr
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T263 Effects of leaf meal of Brouss
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T271 The effects of feeding expandi
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Lactation Biology PostersT278 Effec
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Physiology and Endocrinology Poster
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T288 Effect of Aspergillus meal pre
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Poultry Physiology, Endocrinology,
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T301 Observation of the feeding man
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T307 Effect of levels of Yucca schi
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T313 Study of lysine requirement of
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energy, 5, 26energy and nutrient di
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protein digestive enzyme, 44protein
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HHai, Y., T222, T248Hai-Ying, Z., T
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Song, X., T223Song, Z. G, M144, T20
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102NOTES