Inaugural ASAS–CAAV Asia Pacif ic Rim Conference Abstracts

82 Dietary zinc glycine chelate on growth performance andhematological and immunological characteristics in weanling piglets.Y. Wang, W. Ma, H. Niu, Y. Zhou, and J. Feng*, College of Animal Science,Zhejiang University, Hangzhou, Zhejiang Province, China.The purpose of the study was to find out the effects of dietary zinc glycinechelate on growth, hematological, and immunological characteristics inweanling piglets. A total of one hundred twenty 21-d-old crossbred piglets(Duroc × Landrace × Yorkshire) were randomly allotted to 4 treatments with 3replicate pen of 10 piglets for 35 d. Treatments consisted of 0, 50, and 100 mg/kg of Zn as zinc glycine chelate or 3,000 mg/kg of Zn as zinc oxide(calculatedwith Zn). On d 35 of the feeding trial, 12 pigs (one pig per pen) were humanelykilled. Serum and spleen samples were collected and immediately stored at–70°C until analysis. Small intestine was rinsed with saline to remove thedigesta and then stored in plastic jars containing 100 mL of formalin and thenanalyzed for cell apoptosis and IgA in intestinal mucosa with the TUNEL andABC-ELISA. Spleen was analyzed for expression level of IL-2 mRNA withfluorescence quantitative PCR. All data were analyzed by ANOVA using theGLM procedures of SAS (6.02) for a randomized complete block design.Results of the study showed that, compared with the control, average daily gainand average daily intake were improved (P < 0.05) for pigs fed 100 mg/kg of Znfrom zinc glycine chelate or 3,000 mg/kg of Zn from ZnO. Serum total proteinand albumin increased with the increasing dietary Zn-Gly levels and reacheda peak in 100 mg/kg of Zn as Zn-Gly group, and urea nitrogen decreased (P< 0.05). There was continuous positive staining of the control, and the Zn-Glyand ZnO groups had no obvious cell apoptosis in small intestine. Comparedwith the control, the content of IgA in intestinal mucosa and the expressionlevel of IL-2 mRNA in spleen were significantly increased in dietary Zn-Glygroups (P < 0.05). This study indicates that addition with zinc glycine chelatecould improve growth performance and immunological characteristics in pigs.Key Words: zinc glycine chelate, growth performance, immunologicalcharacteristic83 Effects of iron glycine chelate on tissue mineralconcentrations, feces mineral exertion, and liver antioxidant enzymeactivity in broiler chickens. W. Ma, H. Niu, Y. Wang, and J. Feng*, Collegeof Animal Science, Zhejiang University, Hangzhou, Zhejiang Province, China.The purpose of the study was to find out the effects of iron glycine chelateon tissue mineral concentrations, feces mineral excretion, and liver antioxidantenzyme activities of broiler chicks. A total 360 1-d-old commercial broilerchicks (Ross × Ross) were randomly allotted to 6 dietary treatments with 6replicate pen of 10 chicks for 42 d. Treatments consisted of 0, 40, 80, 120,and 160 mg/kg of iron glycine chelate groups (calculated with Fe) and 160mg/kg of iron sulfate group (calculated with Fe). On d 21, d 42 of the feedingtrial, 72 chicks (2 chicks per pen) were humanely killed by cervical dislocation,respectively. Serum, liver, left breast muscle, left tibia, and feces samples werecollected and immediately stored at –70°C until analysis. Samples (liver, tibia,breast muscle, and feces) were ashed, diluted with deionized-distilled water,and then analyzed for minerals (iron, copper, zinc, and manganum) with flameatomic absorption spectrophotometry. Liver homogenates were analyzed forCu/Zn superoxide dismutase and catalase activities. All data were analyzed byANOVA using the GLM procedures of SAS (6.02) for a randomized completeblock design. Addition with 120, 160 mg/kg of Fe as iron glycine chelate or160 mg/kg Fe as iron sulfate enhanced Fe concentration in serum (P < 0.05),liver (P < 0.05), breast (P < 0.05), tibia (P < 0.05), and feces (P < 0.01) at 21and 42 d. There were linear responses to the addition of iron glycine chelatefrom 0 to 160 mg/kg of Fe on Fe concentration in serum (21 d, P = 0.005; 42d, P = 0.001), liver (P = 0.001), breast (P = 0.001), tibia (P = 0.001), and feces(21 d, P = 0.011; 42 d, P = 0.032). Liver Cu/Zn superoxide dismutase activitiesof chicks were increased by addition of 80, 120, and 160 mg/kg of Fe as ironglycine chelate to diets at 42 d. There were no significant differences in livercatalase activities of chicks among the treatments at 21 and 42 d (P > 0.05).This study indicates that addition with iron glycine chelate could improve irontissue storage and antioxidant enzyme activities in chicks.84 Effects of dietary selenomethionine supplementation ongrowth performance, meat quality, and antioxidant properties in yellowbroilers. Zongyong Jiang*, Lihuan Luo, Yingcai Lin, Shouqun Jiang, andGuilian Zhou, Key Laboratory of Animal Nutrition and Feed(South China),Ministry of Agriculture of P. R. China, Institute of Animal Science, GuangdongAcademy of Agricultural Sciences, Guangzhou, Guangdong, P.R. China.To investigate the effects of dietary selenomethionine (Se-Met) supplementationon growth performance, meat quality, and antioxidant property in broilers, eighthundred 42-d-old Lingnan Yellow male broilers were randomly allotted to 5dietary treatments with 4 replicates per treatment (40 birds per replicate) fora period of 3 wk ad libitum. The control group was fed the basal diet withoutselenium supplementation. The basal diet was supplemented with 0 mg/kg(control), 0.075 mg/kg, 0.15 mg/kg, and 0.225 mg/kg Se from selenomethionine(Se-Met), or 0.15 mg/kg of Se from sodium selenite (SS), respectively. FinalBW and BW gain of birds significantly increased by Se-Met supplementationat the 0.225 mg/kg level (P < 0.05). The addition of Se-Met significantlydecreased drip loss, L* value, and elevated pH value of meat (P < 0.05). Addingsodium selenite (SS) only increased pH value of meat (P < 0.05). In plasma,supplemental Se-Met at 0.225 mg/kg level increased total antioxidant capability(T-AOC), glutathione peroxidase (GPX), total superoxide dismutase (T-SOD),catalase (CAT) activities, and glutathione (GSH) concentration (P < 0.05)and decreased malondialdehyde production (P < 0.05). In breast muscle, theaddition of Se-Met significantly elevated T-AOC, GPX, T-SOD, CAT activities,and contents of metallothionein and GSH (P < 0.05) and reduced carbonylprotein content (P < 0.05). When compared with SS diet, supplemental 0.225mg/kg of Se-Met increased T-AOC, GPX, CAT activities, and GSH content(P < 0.05). Therefore, dietary Se-Met supplementation could improve growthperformance and meat quality by enhancing antioxidative capacity in broilerscompared with SS.Key Words: antioxidation, selenomethionine, yellow broiler85 Long-term performance of commercial laying hens fed dietsdeficient in available phosphorus supplemented with different amounts ofinorganic phosphate or an Escherichia coli-derived phytase (OptiPhos). C.D. Mateo 1 , S. Y. Shen* 2 , N. R. Augspurger 2 , and S. D. Frankenbach 3 , 1 Animal& Dairy Sciences Cluster, University of the Philippines, Los Banos, College,Laguna, Philippines, 2 JBS United, Sheridan, Indiana, USA, 3 Enzyvia, LLC,Sheridan, Indiana, USA.A long-term (40 wk) laying hen feeding trial was performed to study theefficacy of an Escherichia coli-derived phytase (OptiPhos, Enzyvia LLC) forameliorating dietary phosphorus (P) deficiency. Hens were fed diets deficient inavailable phosphorus (0.12% aP) but supplemented with 100, 150, or 250 FTU/kg of OptiPhos, or 0.10 or 0.33% aP from monocalcium phosphate. Experimentaldiets were formulated to meet published requirements for all nutrients except aP.Hens were provided ad libitum access to experimental diets from 20 to 60 wkof age. Hens were individually caged, and 4 consecutive cages were consideredone replicate; each treatment was replicated 8 times. Hens fed the aP-deficientdiet (0.12% aP) without phytase or inorganic P (iP) supplementation had thelowest hen-day egg production (P < 0.05) and the lowest feed intake (P < 0.05).Overall, OptiPhos supplementation at all inclusion rates improved hen-day eggproduction (P = 0.06), feed intake (P < 0.05), and feed conversion ratio (P< 0.05). No further positive impact was observed at OptiPhos inclusion rateshigher than 100 FTU/kg. Addition of 0.10 or 0.33% aP to the aP-deficient dietimproved feed intake (P < 0.05) and tended to improve feed conversion ratio (P= 0.08). A significant improvement in hen-day egg production with additional iPwas observed only in the early stage (P < 0.05), but not in the overall period (P= 0.11). Based on the current trial, supplementing a laying hen diet (0.12% aP)with OptiPhos at 100 to 150 FTU/kg, or 50 to 75 g/t ameliorated the negativeimpact of aP deficiency in commercial laying hens.Key Words: available phosphorus, phytase, laying henKey Words: iron glycine chelate, mineral content, antioxidant enzyme26