NSERC grants at Laurentian University Subventions du CRSNG `a l ...
NSERC grants at Laurentian University Subventions du CRSNG `a l ...
NSERC grants at Laurentian University Subventions du CRSNG `a l ...
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61Sabine Montaut with/avec Nelson Belzile & Yuwei ChenHigh Performance Liquid Chrom<strong>at</strong>ograph for the separ<strong>at</strong>ionof n<strong>at</strong>ural pro<strong>du</strong>ctsA grant of $75,989.Une subvention de 75 989 $.Research Tools and Instruments – <strong>Subventions</strong> d’outils etd’instruments de rechercheThe High Performance Liquid Chrom<strong>at</strong>ograph is a key instrument th<strong>at</strong>will allow us to analyze and separ<strong>at</strong>e small and large amounts of a wide varietyof n<strong>at</strong>ural pro<strong>du</strong>cts and mixtures of compounds. The mixture travelsthrough a column under pressure containing a st<strong>at</strong>ionary phase. For a givenmobile phase, the pro<strong>du</strong>cts are eluted according to their polarities. Thediode-array detector <strong>at</strong>tached to the instrument visualizes the effectivenessof the separ<strong>at</strong>ion and provides structural inform<strong>at</strong>ion. After cre<strong>at</strong>ing our ownpro<strong>du</strong>ct-separ<strong>at</strong>ion protocols, this autom<strong>at</strong>ed instrument will acquire, integr<strong>at</strong>eand store all the d<strong>at</strong>a. The High Performance Liquid Chrom<strong>at</strong>ographis essential for plant extract analysis, n<strong>at</strong>ural pro<strong>du</strong>ct separ<strong>at</strong>ion and quantific<strong>at</strong>ionof n<strong>at</strong>ural pro<strong>du</strong>cts and new biologically active metabolites in plantextracts. A library of known compounds will be cre<strong>at</strong>ed so th<strong>at</strong> they can beeasily identified in complex mixtures.We will use this instrument to investig<strong>at</strong>e the phytochemical compositionof wild cruciferous plants th<strong>at</strong> have not been screened for potential cancerchemoprotection or antioxidant properties. In fact, cancer chemopreventiveactivity of cruciferous vegetables (broccoli, cabbage, cauliflower, kale, Brusselssprouts) is believed to be <strong>du</strong>e to sulfur-containing metabolites (glucosino1<strong>at</strong>es,isothiocyan<strong>at</strong>es), flavonoids and other phenolic compounds. TheHigh Performance Liquid Chrom<strong>at</strong>ograph will also be used to screen endemicplants of Canada, plants used in folk medicine, plant families alreadyknown to possess n<strong>at</strong>ural cancer chemopreventive agents or very promisingnew candid<strong>at</strong>es.The research is expected to give a better understanding of the rel<strong>at</strong>ivecontributions of various components of these plants to cancer risk re<strong>du</strong>ction,and provide fresh avenues for developing novel anti-cancer drugs. Finally, our
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