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Hindi - Himachal Pradesh

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– Enumeration of bacteria responsible for certain primary diseases.3. BACTERIAL STAINING AND CULTIVATION– Microscopy : types and principles– Staining : principles– Media for growth / bacterial colony– Familiarization with materials used4. COMMON TESTS FOR BACTERIAL IDENTIFICATION– Various types of staining such as simple, differential staining; different procedures ofstaining and their principles– Motility testing– Common sugar fermentation and other biochemical tests such as Catalase / Coagulase/citrate utilization/ nitrate reduction / urease/ PPA/ OF/ Indole etc.– Sensitivity testing5. INTRODUCTION TO PARASITOLOGY– Biology of protozoa– Protozoan parasites causing human infection– Medically important helminths– Ectoparasites6. INTRODUCTION TO VIROLOGY– The nature and properties of viruses– Classification of viruses– Morphology7. LABORATORY DIAGNOSIS OF VIRAL INFECTION– Brief appraisal of pathogenicity of viruses– Culture methods– Cytopathic effects– Inclusion bodies– Animal inoculation– Serological test (CFT, HAI, neutralisation)8. INTRODUCTION TO MYCOLOGY– Nature of fungi: basic structures and classification– Superficial mycoses– Subcutaneous mycosis– Systemic fungal infections with opportunistic mycosis9. COMMON LABORATORY METHODS FOR DIAGNOSIS OF FUNGALINFECTIONS(i) KOH preparation with principles(ii) Lactophenal cotton blue preparation(iii) Negative staining and procedures(iv) Special staining and procedures(v) Culture of fungi(vi) Serodiagnosis10. COLLECTION & TRANSPORT OF SAMPLES– Collection of clinical samples– Transport of various appropriate clinical samples.

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