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Name (Title):
Akiyoshi Taniguchi (Group Leader)
Satoshi Migita (NIMS Post doc.)
Affiliation:
International Center for Materials Manoarchitectonics (MANA),
Biomaterials Center, NIMS
Address:
1-1 Namiki, Tsukuba, Ibaraki 305-0044, Japan
Email: TANIGUCHI.Akiyoshi@nims.go,jp
Home Page:
Presentation Title:
Live cell-based sensor devices
Abstract:
Living cells maintain life functions by responding quickly and with great sensitivity to
changes in the external environment. Consequently, sensors using cells as active elements are
thought to be able to perform analyses faster and with more sensitivity than previously possible.
We report here a live cells-based sensorchip fabricated in microfluidic channels in which
several laminar flows were achieved. In addition, we established a cytotoxic sensor cell line,
which was transfected with a green fluorescence protein (GFP) plasmid derived from the heat
shock protein 70B’ (HSP70B’) promoter, which is induced by cytotoxic reagents [1-4]. The
fluorescence in the sensor cells increased in a CdCl2 dose-dependent manner in the microfluidic
channels [5,6]. In this system, cytotoxic reagents can be quantitatively detected in a quick,
sensitive and high- throughput manner. The combination of sensor cells and microfluidic systems
will provide an important basis for the development of micro-total analysis systems (micro-TAS)
technology, and can be applied to toxicology, environmental assessment and drug screening.
98
Fig.1 Cells-based sensorchip fabricated in microfluidic channels.
References:
[1] Okuda-Shimazaki J, Taniguchi A. et al., Open Biotechnol J 2007; 1:1-4.
[2] Wada KI, Taniguchi A, Xu LM, Okano T. Biotechnol Bioeng 2005; 92: 410-5.
[3] Wada KI, Taniguchi A, Okano T. Biotechnol Bioeng 2007; 97,871-876.
[4] Wada KI, Okuda J, Taniguchi A. Open Biotechnol J 2007; 1, 21-25
[5] Okuda-Shimazaki J, Wada KI, Taniguchi A. Open Biotechnol J 2007; 1, 31-33
[6] Wada KI, Taniguchi A, Okano T. Biotechnol Bioeng 2008; 99, 1513-1517.
Poster Session PB-4