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Name (Title):
Xiaofei Yuan(Dr.), Keitaro Yoshimoto(Lecturer), Yukio Nagasaki(Prof.)
Affiliation:
1
Graduate School of Pure and Applied Sciences, University of Tsukuba,
2
Tsukuba Research Center for Interdisciplinary Materials Science (TIMS),
3
Satellite Laboratory of International Center for Materials Nanoarchitechtonics
(MANA) in National Institute for Materials Science (NIMS)
Address: Ten-noudai 1-1-1, Tsukuba, Ibaraki 305-8573, Japan
Email: yuan@ims.tsukuba.ac.jp
Home Page:
Presentation Title:
Construction of a antiferritin/mixed-PEG coimmobilized soft surface onto polystyrene
nanospheres to improve its immunodiagnosis performance
Abstract:
Hydrophilic, flexible and neutral poly(ethylene glycol) (PEG) has been widely used to
stabilize various nano- and micro-scale particles. In addition, since a PEG chain possesses low
affinity for various biomacromolecules, a PEGylated surface improves the performance of
biodiagnosis, especially in immunodiagnostic systems. In this case, the long chain length and
high chain density of the PEG layer were both required, but increased polymer chain length is
always accompanied by decreased polymer chain density,
owing to the steric exclusion of the tethered PEG chains. 1
According to our previous investigations of PEGylated
surfaces, a mixed-PEG chain layer with both long-chain
PEGs and short-chain ones resolved this trade-off relation,
in that the long PEG chains retained the chain length of the
layer, while the short ones easily occupied the inter-space
between two long chains to remarkably increase the total
chain density of the layer. 2 (Scheme 1) Besides this efficient
suppression of various nonspecific interactions, a PEG layer
may also create a suitable environment for specific types of
bio-recognition. 3 Consequently, a biomacromolecule/PEG,
especially a biomacromolecule/mixed-PEG coimmobilized
soft surface is considered to be an ideal surfacemodification
technique for various biosensors, biodiagnosis
systems, and so on.
In this study, an antiferritin/mixed-PEG coimmobilized soft surface was constructed onto
polystyrene nanospheres, in order to solve the problems on conditional BSA blocking treatment.
Though a nanosphere/antiferritin/mixed-PEG (LAmP) complex possessed the size and
distribution similar with those of a nanosphere/antiferritin/BSA (LAB) complex, it showed a
significantly higher immune response yield and a lower detection limit than the LAB complex in
100 % fetal bovine serum (FBS) solution, clearly demonstrating the usefulness of this
PEGylation treatment in construction of efficient biodiagnosis systems.
* Yuan xiaofei, Yoshimoto Keitaro and Nagasaki Yukio, Anal. Chem. in press.
References :
[1] Otsuka, H.; Nagasaki, Y.; Kataoka, K. Biomacromolecules 2000, 1, 39.
[2] Uchida, K.; Otsuka, H.; Kaneko, M.; Kataoka, K.; Nagasaki, Y. Anal. Chem. 2005, 77, 1075.
[3] Yuan, X.; Iijima, M.; Oishi, M.; Nagasaki, Y. Langmuir 2008, 24, 6903.
102
Short
PEG chain
Poster Session PB-8
Nonspecific
adsorption Denaturation
Antibody/PEG coimmobilized soft surface
Nonspecific
adsorption
inhibition
Antibody/mixed-PEG coimmobilized soft surface
Long
PEG chain
Scheme 1. Antibody/PEG (top) and
antibody/mixed-PEG (bottom) soft
surfaces.