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Name (Title):
Junko Okuda-Shimazaki
Affiliation:
International Center for Materials Nanoarchitectonics
(MANA), Biomaterials center, NIMS
Address:
1-1 Namiki, Tsukuba. Ibaraki 305-0044, Japan
Email: OKUDA.junko@nims.go.jp
Home Page:
Presentation Title:
Evaluation of nanomaterials based on molecular biology
Abstract:
The safety and risk evaluation of nanomaterials has gotten a lot
attention recently. Nanomaterials are expected to have the novel
physicochemical properties because of their size, chemical
composition, surface structure, shape, or aggregation. The novel
properties of nanomaterials raise concerns about adverse effects on
biological systems. Some recent studies suggest that nanomaterials
have potential toxicity and that they affect biological behaviors 1-3 .
In this study, nanomaterials, fullerene nanomaterials and titania
were investigated about their biocompatibility by molecular
biological technique. In order to evaluate the cell response to
nanomaterials, we analyze gene expression of biomarkers focused on
stress and toxicity.
Fullerene nanowhiskers (FNWs) were prepared using the liquid -
Poster Session PB-7
Inflammation
liquid interfacial precipitation method 4 that layers poor solvent of fullerene (isopropanol) on a
fullerene solution prepared by pyridine or toluene.
Human acute monocytic leukemia cell line, THP-1 cells were treated with phorbol 12myristate
13-acetate (PMA) 5 . PMA treated THP-1 or after 24 h from seeding NCI-H292, human
mucoepidermoid carcinoma cell line, were exposed by nanomaterials solvent. Total RNA from
nanomaterials exposed cells were isolated and reversibly transcribed with random hexamer
primer. Real time PCR was performed using specific primer for biomarkers or GAPDH as
internal control.
RNA expression analysis showed some biomarkers related to inflammation were induced by
nanomaterials (Fig.). FNT slightly induced stress markers, however its inducing level was lower
than other nanomaterials.
References:
[1] R. F. Service. Science 304 (2004) 1732.
[2] G. Oberdörster, E. Oberdörster, J. Oberdörster. Environ. Health Perspect. 113 (2005) 823.
[3] C. A. Poland, R. Duffin, I. Kinloch, A. Maynard, W.A. H. Wallace, A. Seaton, V. Stone, S.
Brown, W. Macnee, K. Donaldson. Nature Nanotechnology, (AOP) (2008).
[4] K. Miyazawa, Y. Kuwasaki, A. Obayashi, J. Mater. Res. 17 (2002) 83.
[5] J. Okuda-Shimazaki, A. Yamamoto, D. Kuroda, T. Hanawa, A. Taniguchi, Open Biotechnol.
J. 1 (2007) 14.
vs. GAPDH
0.005
0.004
0.003
0.002
0.001
0
0.001 % TiO 2 (� (��� �� = 500 nm)
0.001 % TiO 2 (� (���= ��= 170 nm)
dH O 2
Fig. gene expression of titania
exposed cells
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