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Name (Title):
Jun Nakanishi (MANA Independent Scientist)
Affiliation:
International Center for Materials Nanoarchitectonics (MANA), NIMS
Address:
1-1 Namiki Tsukuba, Ibaraki 305-0044, JAPAN
Email: NAKANISHI.Jun@nims.go.jp
Home Page: http://www.nims.go.jp/mana/members/young_scientist/j_nakanishi/index.html
Presentation Title:
Photoresponsive Biointerfaces for Cell Analysis
Abstract:
Biointerface is an interface between biomolecules and materials. We have been developing
photoresponsive biointerfaces for controlling cellular functions as well as for engineering tissue
mimics in vitro.
Photoactivatable cell culture substrate. [1] Activities of the cells are highly dependent on their
microenvironment, as can be imagined from the complex architecture of tissues and organs.
However, most cell researches have been conducted on normal plastic or glass dish, whereon
cells were attached quite randomly. We have developed several photoactivatable cell culture
substrates that changed from non-cell-adhesive to cell adhesive in response to light (Figure 1a).
Based on this feature, we were able to produce arbitral cell patterns by controlled irradiation.
Furthermore, we succeeded in inducing cell migration and proliferation by irradiation during cell
cultivation.
Photoresponsive nanocarrier. [2] Concentrations of biomolecules such as hormones and proteins in
living system are strictly controlled both in time and space. Therefore, it is important to deliver
such biomolecules at will not only for therapeutic purposes but also for studying intertwined
inter- and intracellular signal transduction networks. We have developed colloidal gold
nanoparticles (GNPs) presenting a photocleavable succinimidyl ester that allows for the delivery
of amine derivatives (Figure 1b). Under this molecular design, the GNPs capture the amine
derivatives on their surfaces and release them upon irradiation at any desired time and space. As a
proof of concept, we synthesized histamine-immobilized GNPs and examined their ability to
evoke intracellular signaling by fluorescence imaging.
(a) O
(b)
PEG NH2-PEG Non cell-adhesive Cell-adhesive
Capture Release
Figure 1. Photoresponsive Biointerfaces. (a) Change in cell adhesiveness on a photoactivatable cell culture substrate.
(b) Capture and release of amine derivatives (NH2-R) on photoresponsive gold nanoparticles (GNPs).
References:
[1] J. Nakanishi et al. J. Am. Chem. Soc., 129, 6694, 2007; Y. Kikuchi et al. Chem. Lett., 37,
1062, 2008.
[2] J. Nakanishi et al. submitted.
14
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Oral Presentation 14
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