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Clinical evaluation of serum Alcohol Dehydrogenase activity in horses with acute intestinal obstruction Naglaa Gomaa, Gábor Köller, and G.F Schusser Department of Large Animal Medicine, Faculty of Veterinary Medicine, University of Leipzig Introduction: Intestinal obstruction is an abdominal emergency in horses. Survival and outcome of these cases is very important especially for the owners due to the high costs of surgery and intensive care. Several biochemical parameters were used as prognostic indicators of intestinal ischemia but they lack specificity and sensitivity. Although the liver seems to be the first organ after intestine that affected by intestinal ischemia as the hepatic vasculature is in series with intestinal circulation, the lack of an early discriminating hepatic function test delays recognition of hepatic failure. Serum ADH is a cytosolic hepatic enzyme, abundant in centrilobular region of the liver acinus and consi<strong>der</strong>ed a more sensitive biochemical marker of hepatic hypoxia due to vascular disturbance in the liver. In this study, we aimed to evaluate ADH activity in horses with acute intestinal obstruction and to determine whether the level of serum ADH activity in these horses has a diagnostic and prognostic value. Material and method: 30 clinically healthy horses were used as a control group for measuring serum ADH activity in normal adult horses. A prospective study included only 64 horses with acute intestinal obstruction was carried out. The diagnoses was done by rectal examination and confirmed after surgical or pathological findings. The horses were divided into 3 groups: G1 (non-strangulation obstruction) included 23 horses with impaction of left ventral colon and left colon displacement, G2 (small intestinal strangulation) included 22 horses with inguinal hernia, lipoma pendulus, volvulus and intestinal incarceration and G3 (colonic torsion) included 19 horses with partial and total colon torsion. Spectrophotometric assay of serum ADH activity in healthy horses and all groups of horses with colic was carried out using Beckman Coulter Spectrophotometer. In addition, serum lactate concentration, Aspartataminotransferase (AST), Gammaglutamyltransferase (GGT), Glutamatdehydrogenase (GLDH) activities were measured using an automatic analyzer (Hitachi 912, Boehringer Mannheim). The data was statistically analyzed using SPSS 15 software programme. Diagnostic or prognostic cut-off values of ADH activity for distinguishing intestinal strangulation from non strangulation obstruction or predicting the outcome of horses
equired surgical treatment respectively was determined using Win Episcope 2 software programme. Results: Median serum ADH activity in healthy horses was 10.5 U/l. It was significantly increased (P< 0.05) in all groups of horses with acute intestinal obstruction (G1= 17.5 U/l, G2= 40 U/l, G3= 63.2 U/l). AST and GLDH activities were only significantly increased in group3 in comparison to other groups while GGT activity did not show any significant difference between all groups. There was a positive significant correlation between ADH activity and lactate concentration in serum in group 1 and 3 (P
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