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CHAPTER 7 Miscellaneous Protozoa

common name, and morphologic form,

with justification.

B. Identify the diseases and conditions

associated with the responsible parasite

C. Construct a life cycle associated with

each organism present that includes

corresponding epidemiology, route of

transmission, infective stage, and

diagnostic stage.

D. Propose each of the following related to

stopping and preventing further parasite

infections:

1. Treatment options

2. Prevention and control plan

E. Determine the specimen(s) of choice and

alternative specimen types, when

appropriate, as well as appropriate

laboratory diagnostic techniques for the

recovery of each responsible parasite.

F. Recognize sources of error including

but not limited to those involved in

specimen collection, processing, and

testing and propose solutions to remedy

them.

G. Interpret laboratory data, determine

specific follow-up tests to be done, and

predict the results of those identified

tests.

H. Determine additional morphologic

forms, where appropriate, that may also

be detected in clinical specimens.

7-11 Identify, compare, and contrast the

similarities and differences among the

parasites discussed in this and the other

chapters in this text.

7-12. Describe standard, immunologic, and new

laboratory diagnostic approaches for the

recovery of the miscellaneous Protozoa in

clinical specimens.

7-13. Given prepared laboratory specimens, and

with the assistance of this manual, the

learner will be able to:

A. Differentiate the protozoan organisms

from artifacts and other parasites.

B. Correctly name each protozoan parasite

based on its key characteristic

structure(s).

CASE STUDY 7-1

UNDER THE MICROSCOPE

Joseph, a 29-year old man, went to a local health clinic

with complaints of intermittent diarrhea for a few months

and trouble sleeping. A stool specimen was collected and

preserved in 10% formalin and PVA. An O&P examination

was performed, along with a permanent trichrome stain.

The stain revealed the organism shown below that measured

10 µm in diameter, with a large, centrally located

vacuole and small red nuclei within the peripheral ring of

cytoplasm.

Questions and Issues for Consideration

1. What parasite do you suspect, and why? (Objectives

7-10A)

2. Why do false-negative results sometime occur with this

organism? (Objective 7-10F)

3. How does the organism reproduce? (Objective 7-10C)

4. Describe the controversy surrounding the classification

of this organism. (Objective 7-3)

5. Discuss treatment and prevention measures. (Objective

7-10D)

Size range: 5-32 m

Average size: 7-10 m

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