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CHAPTER 3 The Amebas

diagnostic technique(s) required for the

recovery of each of the amebas.

3-9. Given the name, description,

photomicrograph, and/or diagram of an

ameba:

Identify, describe and/or label its

characteristic structures, when appropriate

State the purpose of the designated

characteristic structure(s).

Name the parasite, including its

morphologic form,

3-10. Analyze case studies that include pertinent

patient information and laboratory data

and:

A. Identify and describe the function of

key differential characteristics structures.

B. Identify each responsible amebic

organism by category, scientific name,

common name, and morphologic form,

with justification when indicated.

C. Identify the associated symptoms,

diseases, and conditions associated with

the responsible parasite.

D. Construct a life cycle associated with

each amebic parasite present that

includes corresponding epidemiology,

route of transmission, infective stage,

and diagnostic stage(s).

E. Propose each of the following related to

stopping and preventing ameba

infections:

1. Treatment options

2. Prevention and control plan

F. Determine the specimen(s) of choice and

alternative specimen types, when

appropriate, as well as the appropriate

laboratory diagnostic techniques for the

recovery of each ameba.

G. Recognize sources of error, including

but not limited to those involved in

specimen collection, processing, and

testing and propose solutions to remedy

them.

H. Interpret laboratory data, determine

specific follow-up tests that could or

should be done, and predict the results

of those identified tests.

I. Determine additional morphologic

forms, when appropriate, that may also

be detected in clinical specimens.

3-11. Compare and contrast the similarities and

differences between:

A. The amebas covered in this chapter

B. The amebas covered in this chapter and

the other parasites covered in this text.

3-12. Describe the standard, immunologic, and

new laboratory diagnostic approaches for

the recovery of amebas in clinical

specimens.

3-13. Given prepared laboratory specimens, and

with the assistance of this manual, the

student will be able to:

A. Differentiate amebic parasites from

artifacts.

B. Differentiate the amebic organisms

from each other and from the

other appropriate categories of

parasites.

C. Correctly identify each amebic parasite

by scientific, common name, and

morphologic form based on its key

characteristic structure(s).

CASE STUDY 3-1

UNDER THE MICROSCOPE

Eleven-year-old Scooter was admitted to the emergency

room with complaints of fever, vomiting, and altered

mental status. Patient history revealed that the boy felt

fine until 2 days ago, at which time he developed a headache

and experienced photophobia. On questioning

Scooter, it was discovered that the patient went swimming

in a local river 5 days ago. A lumbar puncture was

attempted but was unsuccessful because of the child’s

combativeness. Antibiotics were administered because of

suspected meningitis. The patient was then transferred to

a tertiary care facility for further evaluation. He suffered a

seizure en route, with jerking movements of his right side.

A complete blood count was done, which revealed an

increased white blood cell count with a predominance of

segmented neutrophils. A second lumbar puncture was

attempted at the tertiary care facility. The successful puncture

revealed a yellowish, puslike fluid. The spinal fluid’s

protein, white blood cell, and red blood cell levels were

elevated, with a decrease in glucose levels. Examination

of the spinal fluid wet mount actively showed motile

trophozoites. Within 3 days of being transferred to the

tertiary facility, Scooter slipped into a coma and never

regained consciousness. He died 5 days after onset of the

illness.

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