[Elizabeth_Zeibig]_Clinical_Parasitology__A_Practi(z-lib.org)

C H A P T E R
2
Specimen Collection and
Processing
Lauren Roberts and Elizabeth Zeibig
W H A T ’ S A H E A D
Focusing In
Stool for Ova and Parasite
Examination
Stool Screening Methods
Other Intestinal Specimens
Other Specimens and
Laboratory Techniques
Immunologic Testing
Reporting of Results and
Quality Control
Looking Back
L E A R N I N G O B J E C T I V E S
On completion of this chapter and review of its
tables and ocular micrometer diagram, the
successful learner will:
2-1. Define the following key terms:
Concentration technique (pl., techniques)
Concentrated iodine wet preparation
(pl., preparations)
Concentrated saline wet preparation
Concentrated wet preparation
Direct iodine wet preparation
Direct saline wet preparation
Direct wet mount (pl., mounts)
Direct wet preparation
Fixative (pl., fixatives)
Micron (pl., microns; abbreviated µ or µm)
O&P
Ocular micrometer
Ova and parasites
Permanent stained smear (pl., smears)
Preparation, prep (pl., preparations;
abbreviated as preps)
Unfixed
2-2. Identify and describe the proper collection
and transport of stool samples for parasitic
study.
2-3. Identify the procedures included in a
routine O&P examination.
2-4. Discuss the basic composition, purpose,
advantages, and disadvantages of each of
the following preservatives and state which
laboratory procedures can be performed
using each type:
A. Formalin
B. Polyvinyl alcohol (PVA)
C. Sodium-acetate formalin (SAF)
D. Modified PVA
E. Alternative single-vial systems
2-5. Describe the characteristics of the
macroscopic examination of stool
specimens.
2-6. Identify the procedures involved in the
microscopic examination of stool
specimens.
2-7. State the purpose and procedure involved
when calibrating and using an ocular
micrometer.
2-8. Describe the use of a direct wet
preparation and state when this procedure
can be eliminated from an O&P
examination.
14 Copyright © 2013 by Saunders, an imprint of Elsevier Inc.