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[Elizabeth_Zeibig]_Clinical_Parasitology__A_Practi(z-lib.org)

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CHAPTER 3 The Amebas

73

3-4. Classify the individual ameba as intestinal

or extraintestinal. (Objective 3-4)

Entamoeba histolytica

Entamoeba hartmanni

Entamoeba coli

Entamoeba polecki

Endolimax nana

Iodamoeba bütschlii

Entamoeba gingivalis

Naegleria fowleri

Acanthamoeba species

___________________

___________________

___________________

__________________

__________________

__________________

__________________

__________________

__________________

3-5. Describe the typical life cycle of an intestinal

ameba. (Objective 3-5 A)

3-6. Compare the life cycles of Entamoeba

gingivalis and Naegleria fowleri. (Objective

3-5C)

3-7. Amebic dysentery is caused by: (Objective

3-6)

A. Entamoeba histolytica

B. Entamoeba coli

C. Entamoeba gingivalis

D. Endolimax nana

3-8. Identify and describe the clinical significance

associated with each of the following

amebas. (Objective 3-6)

A. Entamoeba histolytica

B. Entamoeba coli

C. Naegleria fowleri

D. Acanthamoeba species

3-9. True/False. Treatment for patients with

asymptomatic intestinal amebiasis is not

recommended. (Objective 3-7)

A. True

B. False

3-10. Primary amebic meningoencephalitis is

primarily caused by: (Objective 3-6)

A. Entamoeba histolytica

B. Naegleria fowleri

C. Entamoeba gingivalis

D. Acanthamoeba spp.

3-11. Match each ameba (column A) with the

corresponding specimen of choice for its

recovery (column B). (Objective 3-8)

Column A

Column B

1. Entamoeba histolytica ___ A. Stool

2. Entamoeba coli ___ B. Spinal fluid

3. Endolimax nana ___ C. Mouth

scrapings

4. Entamoeba gingivalis ___ D. Corneal

scrapings

5. Acanthamoeba spp.

6. Naegleria fowleri

3-12. Describe the standard laboratory approach

for the recovery of amebic organisms.

(Objective 3-12)

CASE STUDY 3-2

UNDER THE MICROSCOPE

Annie, an 11-year-old white girl, was adopted from a

downtown Chicago orphanage and brought home to her

new family in rural Illinois. Three weeks later, she began to

experience severe diarrhea, fever, malaise, and abdominal

cramping. Examination by her pediatrician led him to order

a stool for culture and parasite examination (commonly

known as an ova and parasite examination [O&P]). The

stool arrived in the laboratory and the gross appearance

was noted as liquid, with mucus and tinges of blood. It was

immediately processed by standard techniques (discussed

in detail in Chapter 2). The culture was negative for intestinal

pathogens.

The direct and concentrated saline wet preps slide

showed suspicious, irregularly shaped forms. The laboratory

technician on duty, suspicious but not certain that a

parasite was present, decided to hold the report pending

further studies. The suspicious form seen in the permanent

stain is shown in the diagram. This form measured 13 µm.

Continued

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