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Proceedings e report - Firenze University Press

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4. Conclusions<br />

DEGRADATION OF MELANIN AND BIOCIDES BY LIGNINOLYTIC FUNGI<br />

These procedures look very promising, but they need to be optimized and the integrity (or possible<br />

damage) of paper samples after the procedure should be carefully evaluated. After scientifically<br />

verifying that the melanin degradation procedure is not causing damage to the paper, and that the<br />

procedure fulfils paper conservator’s requirements, it will be introduced in conservation of invaluable<br />

heritage.<br />

Lindane and PCB showed no significant influence on fungal growth, while the growth rate in the<br />

presence of PCP was unaffected only in the case of T. versicolor. Among tested fungi, P. ostreatus<br />

most efficiently degraded PCB and lindane. Lindane was degraded in minor extent by this white-rot<br />

fungs most likely because of the fact that its does not have the aromatic character. Surprisingly,<br />

despite the fact that PCP had been used as a broad spectrum fungicide in many wood preservatives, it<br />

has been efficiently degraded by T. versicolor. Our results clearly show the promising potential of<br />

white rot fungi in bioremediation of organochlorine biocides and melanin in the field of conservation.<br />

However, the procedures will require adequate equipment for such restoration treatments.<br />

References<br />

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1115-1136.<br />

2. Butler, M.J., and Day, A.W. (1998b): Destruction of fungal melanins by ligninases of<br />

Phanerochaete chrysosporium and other white rot fungi. Int. J. Plant Sci. 159: 989-995.<br />

3. Unger, A, Schniewind, A. P., and Unger, W. (2001): Conservation of Wood Artifacts, Springer-<br />

Verlag, Berlin Heidelberg, Germany.<br />

4. Levin, L., Viale, A., and Forchiassin, A. (2003): Degradation of organic pollutants by the white<br />

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8. Bao, W., Fukushima, Y., Jensen, K. A., Jr., Moen, M. A., and Hammel, K. E. (1994): Oxidative<br />

degradation of non-phenolic lignin during lipid peroxidation by fungal manganese peroxidase.<br />

FEBS Lett., 354(3): 297-300.<br />

9. Moen, M. A., and Hammel, K. E. (1994): Lipid Peroxidation by the Manganese Peroxidase of<br />

Phanerochaete chrysosporium Is the Basis for Phenanthrene Oxidation by the Intact Fungus.<br />

Appl. Environ. Microbiol., 60(6): 1956-1961.<br />

10. Bourbonnais, R, and Paice, M. G. (1990): Oxidation of non-phenolic substrates. An expanded<br />

role for laccase in lignin biodegradation. FEBS Lett., 267(1): 99-102.<br />

11. Eggert, C., Temp, U., Dean, J. F., and Eriksson, K. E. (1996): A fungal metabolite mediates<br />

degradation of non-phenolic lignin structures and synthetic lignin by laccase. FEBS Lett., 391(1-<br />

2): 144-148.<br />

12. Reddy, C. A. and Mathew, Z. (2001): Bioremediation potential of white rot fungi. Fungi in<br />

bioremediation. Cambridge <strong>University</strong> <strong>Press</strong>, Cambridge, U.K.<br />

134

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