Impact Of Host Plant Xylem Fluid On Xylella Fastidiosa Multiplication ...

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Table 1. Results of targeted dissections of internal reproductive morphology of H. coagulata reared under several photoperiod and temperature regimes. Photoperiod and Temperature a n Ovarioles Ova Fat body Brochosomes 13.5:10.5 (Aug 5) 15 2 0 2.5-3 1 23-29 o C 5 3 2 2.5-3 3 13:11 (Aug 24) 18 2 0 3 1 23-29 o C 6 2 0 2.5 1 Greenhouse (May19-Jun29) 15 3 2 2.5-3 3 13h 13m – 14h 5m (photophase) 31-37 o C Greenhouse (Jan6-Feb26) 15 2 0 3 1 10h 16m – 11h 16m (photophase) 16.7-27.2 o C a Photoperiod and date for latitude of Tallahassee, FL. Key: Ovarioles 1=not developed 2=fully developed; no ova 3=fully developed with ova Fat body 1=minimal 2=medium 3=heavy Ova 0=none 1=single ova per ovariole 2=two ova per ovariole Brochosomes (within Malpighian tubules) 1=small; tubule translucent 2=medium; tubule filled opaque white 3=large; tubule swollen opaque white Parasitism 2004 100 80 % parasitism 60 40 20 0 50 100 150 200 250 300 Julian Date Figure 1. Seasonal parasitism of H. coagulata eggs by Gonatocerus spp. in north Florida. - 155 -
REFERENCES Blua, M. J. and D. J. W. Morgan. 2003. Dispersion of Homalodisca coagulata (Hemiptera: Cicadellidae), a vector of <strong>Xylella</strong> fastidiosa, into vineyards in southern California. J. Econ. Entomol. 96: 1369-1374. Brodbeck, B. V., P. C. Andersen, and R. F. Mizell. 1995. Differential utilization of nutrients during development by the xylophagous leafhopper, Homalodisca coagulata. Entomol. Exp. Appl. 75: 279-289. Lees, A. D. 1966. Photoperiodic timing mechanisms in insects. Nature, Lond. 210: 986-989. Lopez, R., R. F. Mizell III, P. C. Andersen, and B. V. Brodbeck. 2004. Overwintering biology, food supplementation and parasitism of eggs of Homalodisca coagulata (Say) (Homoptera: Cicadellidae) by Gonatocerus ashmeadi Girault and Gonatocerus morrilli (Howard) (Hymenoptera: Mymaridae). Masaki, S. 1980. Summer diapause. Ann. Rev. Entomol. 25: 1-25. Mizell, R. F., and W. J. French. 1987. Leafhopper vectors of phony peach disease: Feeding site preference and survival on infected and uninfected peach, and seasonal response to selected host plants. J. Entomol. Sci. 22: 11-22. Pollard, H. N, and G. H. Kaloostian. 1961. Overwintering habits of Homalodisca coagulata, the principle vector of phony peach disease virus. J. Econ Entomol. 54: 810-811. Turner, W. F. and H. N. Pollard. 1959. Life histories and behaviors of five insect vectors of phony peach disease. USDA Tech. Bull. 1188. 28 pages. FUNDING AGENCIES Funding for this project was provided by the CDFA Pierce’s Disease and Glassy-Winged Sharpshooter Board. - 156 -
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IMPACT OF HOST PLANT XYLEM FLUID ON
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0.18 600 Optical density 0.16 0.14
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OPTIMIZING MARKER-ASSISTED SELECTIO
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esistant and susceptible genotypes
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MAP BASED IDENTIFICATION AND POSITI
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esistant genotypes are in process a
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BREEDING PIERCE’S DISEASE RESISTA
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Progeny from crosses of field resis
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a = (1=low, 4= high); b = (1=green,
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v.8) for differences due to the pla
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SHARPSHOOTER FEEDING BEHAVIOR IN RE
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correlated with all other findings
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EFFECTS OF FEEDING SUBSTRATE ON RET
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REFERENCES Bextine, B. and T. Mille
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will also provide insights into the
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OBJECTIVES 1. Determine glassy-wing
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GWSS per 30 s sweep (seasonal avera
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ELISA for the presence of GWSS egg
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Project Leader: Thomas P. Freeman E
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Freeman, T. P., R. A. Leopold, D. R
Page 45 and 46: pathogen, when they move into viney
Page 47 and 48: A NOVEL IMMUNOLOGICAL APPROACH FOR
Page 49 and 50: 1.6 GWSS Adults That Fed on Protein
Page 51 and 52: Hagler, J.R. & S.E. Naranjo. 2004.
Page 53 and 54: RESULTS: Oviposition Survey Wild gr
Page 55 and 56: Host specificity testing: No-choice
Page 57 and 58: currently employed morphological ch
Page 59 and 60: increase our present understanding
Page 61 and 62: BIOLOGY AND MORPHOMETRIC ANALYSIS O
Page 63 and 64: Table 1. Mean a developmental durat
Page 65 and 66: EFFECTS OF USING CONSTANT AND CYCLI
Page 67 and 68: REFERENCES Gautam, R. D. 1986. Effe
Page 69 and 70: PARASITISM OF THE GLASSY-WINGED SHA
Page 71 and 72: Table 1. Parasitism by G.ashmeadi o
Page 73 and 74: Project Leader: Robert F. Luck Dept
Page 75 and 76: A more interesting analysis using t
Page 77 and 78: MYCOPATHOGENS AND THEIR EXOTOXINS I
Page 79 and 80: POPULATION DYNAMICS AND INTERACTION
Page 81 and 82: No egg masses were recorded on olea
Page 83 and 84: EXPLORATION FOR FACULTATIVE ENDOSYM
Page 85 and 86: Although Baumannia and Wolbachia we
Page 87 and 88: EFFECTS OF SUBLETHAL DOSES OF IMIDA
Page 89 and 90: Table 2. Mortality and flight perfo
Page 91 and 92: A NOVEL METHOD TO INDUCE OVIPOSITIO
Page 93 and 94: REFERENCES Brodbeck, B. V., P. C. A
Page 95: Cohorts H. coagulata neonates were
Page 99 and 100: Figure 2 shows the average numbers
Page 101 and 102: REPRODUCTIVE BIOLOGY AND PHYSIOLOGY
Page 103 and 104: REFERENCES Blua, M. J., Phillips, P
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Page 109 and 110: RESULTS Some plant families had no
Page 111 and 112: Table 5. Winter, spring and summer
Page 113 and 114: 16S rDNA is by far the most sequenc
Page 115 and 116: DNA MICROARRAY AND MUTATIONAL ANALY
Page 117 and 118: Inhibition of biofilm is BSA concen
Page 119 and 120: CULTURE-INDEPENDENT ANALYSIS OF END
Page 121 and 122: Borneman, J., M. Chrobak, G. Della
Page 123 and 124: P (CFU P OBJECTIVE 1. Determine the
Page 125 and 126: Purcell, AH and SR Saunders. 1999a.
Page 127 and 128: Figure 1: Southern blot of tolC::ap
Page 129 and 130: Project Leader: David Gilchrist Dep
Page 131 and 132: III. Production of transgenic plant
Page 133 and 134: RESULTS Construction of cDNA Librar
Page 135 and 136: CONCLUSIONS Genetic resistance and
Page 137 and 138: Mutagenesis of Xylella The EZ::TN T
Page 139 and 140: ISOLATION OF BACTERIOPHAGES SPECIFI
Page 141 and 142: Project Leader: Michele M. Igo Sect
Page 143 and 144: outer membrane fractions using two-
Page 145 and 146: 1995; Purcell and Saunders, 1999).
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DEVELOPMENT OF SSR MARKERS FOR GENO
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Strain Name Host of Origin County o
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ROLE OF ATTACHMENT OF XYLELLA FASTI
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wild-type cells was not conclusive
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DETERMINATION OF GENES CONFERRING H
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S1 S2 S3 S4 Nb123456789bb123456789b
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MULTILOCUS SEQUENCE TYPING TO IDENT
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GENOME-WIDE IDENTIFICATION OF RAPID
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Americas and since most of the plan
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EFFECTS OF CHEMICAL MILIEU ON ATTAC
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CONCLUSIONS Our overall objective i
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RESULTS Objective 1. We conducted t
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Redak, R. A., Purcell, A. H., Lopes
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In parallel, an “activating trans
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PATTERNS OF XYLELLA FASTIDIOSA INFE
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% of vessels 100 80 60 40 20 Mugwor
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DOCUMENTATION AND CHARACTERIZATION
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Magnolia002 showed more identity (9
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PLASMID ADDICTION AS A NOVEL APPROA
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GENETIC VARIABILITY OF XYLELLA FAST
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probing activities). In preliminary
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the slow release valve was opened a
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12. Hopkins DL (1977) Diseases caus
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The almost complete absence of info
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QUANTIFYING LANDSCAPE-SCALE MOVEMEN
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Table 1. The mean (±SD) ELISA read
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EPIDEMIOLOGICAL ASSESSMENTS OF PIER
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multiply to relatively high (easily
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SPATIAL DATABASE CREATION AND MAINT
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Project Leader: Thomas M. Perring D
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Figure 2. Individual leaves from a
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The state variables, process functi
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DEVELOPMENT OF A FIELD SAMPLING PLA
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Figure 1. Three main dispersion pat
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OBJECTIVES 1. Track the movement of
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REFERENCES 1. Beard, C.B., Cordon-R
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cases, positive phloem samples were
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EXPLOITING XYLELLA FASTIDIOSA PROTE
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Figure 2. Polymer disk accumulation
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CHARACTERIZATION OF NEONICOTINOIDS
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EVALUATION OF RESISTANCE POTENTIAL
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Project Leader: Doug Cook Dept. of
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Based on the in silico analysis, de
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CONTROL OF PIERCE’S DISEASE THROU
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Figure 1. Oleander ‘White’ afte
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RESULTS During the reporting period
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DEVELOPMENT OF AN ARTIFICIAL DIET A
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DESIGN OF CHIMERIC ANTI-MICROBIAL P
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Neutrophil elastase Cecropin B Chim
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and WTXb is very low (0.00-0.40%) a
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100 100 0.056 North America 100 0.0
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GENETIC DIFFERENTIATION AMONG GEOGR
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Table 1. Single-populations descrip
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MOLECULAR DISTINCTION BETWEEN POPUL
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These novel observations strongly s
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SEQUENCE DIVERGENCE IN TWO MITOCHON
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Table 3. Pairwise sequence distance
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DEVELOPMENT OF MOLECULAR DIAGNOSTIC
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A. B. Relative Density of SCAR Band
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THE ALIMENTARY TRACK OF GLASSY-WING
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We have dissected and identified al
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REALIZED LIFETIME PARASITISM AND TH
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REPRODUCTIVE AND DEVELOPMENTAL BIOL
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Mean adult longevity (days) 25 20 1
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the proposed exploratory work; thes
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SEARCHING FOR AND COLLECTING EGG PA
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REFERENCES Hoddle, M. S. and S. V.
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some Gram(+) bacteria, but are inac
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7. Hultmark, D., et al., Insect Imm
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Isolation of Fungal Pathogens Soil
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IDENTIFICATION OF MECHANISMS MEDIAT
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concentrations of 1.5 x 10 7 bacter
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anchor it in the outer membrane (sh
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SYMBIOTIC CONTROL OF PIERCE’S DIS
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MANAGEMENT OF PIERCE’S DISEASE OF
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pfF mutants are taken up by insects
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Simpson, A. J. G., F. C. Reinach, P
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Al-Wahaibi, A.K., Owen, and J. G. M
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patterns differed among the lines,
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Punja, Z.K. 2001. Genetic engineeri
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mind, we conducted an additional st
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REFERENCES Toscano, N., Byrne, F.,
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RESULTS AND CONCLUSIONS The program
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COMPATIBILITY OF INSECTICIDES WITH
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More tests are in progress to addre
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Impact Of Host Plant Xylem Fluid On Xylella Fastidiosa Multiplication ...

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