C - Michigan Technological University

Cyclin D1 Gene ExpressionO N D J F M A MMonth of Serum CollectionFigure 6.3—Longitudinal analysis of Cyclin D1 gene expression in undifferentiated cellsstimulated for 3 hours in seasonal bear serumMC3T3-E1 cells were cultured in seasonal bear serum for 3 hours, followed by real-time PCRquantification of relative gene expression. Each point represents the gene expressionresponse from one serum sample. Vertical lines represent transitions between active andhibernating seasons. Samples are from 1 black bear in the 2006/07 season.Per1 Gene ExpressionO N D J F M A MMonth of Serum CollectionFigure 6.4—Longitudinal analysis of Per1 gene expression in undifferentiated cells stimulatedfor 3 hours in seasonal bear serumMC3T3-E1 cells were cultured in seasonal bear serum for 3 hours, followed by real-time PCRquantification of relative gene expression. Each point represents the gene expressionresponse from one serum sample. Vertical lines represent transitions between active andhibernating seasons. Samples are from 2 black bears in the 2006/07 season.103