C - Michigan Technological University

Runx2 Gene ExpressionO N D J F M A MMonth of Serum CollectionFigure 6.57—Longitudinal analysis of Runx2 gene expression in cells differentiated for 6 daysin seasonal bear serumMC3T3-E1 cells were differentiated in seasonal bear serum for 6 days, followed by real-timePCR quantification of relative gene expression. Each point represents the gene expressionresponse from one serum sample. Vertical lines represent transitions between active andhibernating seasons. Samples are from 2 black bears in the 2006/07 season.Smurf1 Gene ExpressionO N D J F M A MMonth of Serum CollectionFigure 6.58—Longitudinal analysis of Smurf1 gene expression in cells differentiated for 6days in seasonal bear serumMC3T3-E1 cells were differentiated in seasonal bear serum for 6 days, followed by real-timePCR quantification of relative gene expression. Each point represents the gene expressionresponse from one serum sample. Vertical lines represent transitions between active andhibernating seasons. Samples are from 2 black bears in the 2006/07 season.130