C - Michigan Technological University

Akt Gene ExpressionO N D J F M A MMonth of Serum CollectionFigure 6.9—Longitudinal analysis of Akt gene expression in differentiated cells stimulated for3 hours in seasonal bear serumDifferentiated MC3T3-E1 cells were cultured in seasonal bear serum for 3 hours, followed byreal-time PCR quantification of relative gene expression. Each point represents the geneexpression response from one serum sample. Vertical lines represent transitions betweenactive and hibernating seasons. Samples are from 2 black bears in the 2006/07 season.BAD Gene ExpressionO N D J F M A MMonth of Serum CollectionFigure 6.10—Longitudinal analysis of BAD gene expression in differentiated cells stimulatedfor 3 hours in seasonal bear serumDifferentiated MC3T3-E1 cells were cultured in seasonal bear serum for 3 hours, followed byreal-time PCR quantification of relative gene expression. Each point represents the geneexpression response from one serum sample. Vertical lines represent transitions betweenactive and hibernating seasons. Samples are from 2 black bears in the 2006/07 season.106