C - Michigan Technological University

More documents

Recommendations

Info

Akt Gene ExpressionO N D J F M A MMonth of Serum CollectionFigure 6.9—Longitudinal analysis of Akt gene expression in differentiated cells stimulated for3 hours in seasonal bear serumDifferentiated MC3T3-E1 cells were cultured in seasonal bear serum for 3 hours, followed byreal-time PCR quantification of relative gene expression. Each point represents the geneexpression response from one serum sample. Vertical lines represent transitions betweenactive and hibernating seasons. Samples are from 2 black bears in the 2006/07 season.BAD Gene ExpressionO N D J F M A MMonth of Serum CollectionFigure 6.10—Longitudinal analysis of BAD gene expression in differentiated cells stimulatedfor 3 hours in seasonal bear serumDifferentiated MC3T3-E1 cells were cultured in seasonal bear serum for 3 hours, followed byreal-time PCR quantification of relative gene expression. Each point represents the geneexpression response from one serum sample. Vertical lines represent transitions betweenactive and hibernating seasons. Samples are from 2 black bears in the 2006/07 season.106
BAK Gene ExpressionO N D J F M A MMonth of Serum CollectionFigure 6.11—Longitudinal analysis of BAK gene expression in differentiated cells stimulatedfor 3 hours in seasonal bear serumDifferentiated MC3T3-E1 cells were cultured in seasonal bear serum for 3 hours, followed byreal-time PCR quantification of relative gene expression. Each point represents the geneexpression response from one serum sample. Vertical lines represent transitions betweenactive and hibernating seasons. Samples are from 2 black bears in the 2006/07 season.BAX Gene ExpressionO N D J F M A MMonth of Serum CollectionFigure 6.12—Longitudinal analysis of BAX gene expression in differentiated cells stimulatedfor 3 hours in seasonal bear serumDifferentiated MC3T3-E1 cells were cultured in seasonal bear serum for 3 hours, followed byreal-time PCR quantification of relative gene expression. Each point represents the geneexpression response from one serum sample. Vertical lines represent transitions betweenactive and hibernating seasons. Samples are from 2 black bears in the 2006/07 season.107
Page 1 and 2:
EXPLORATION OF THE ROLE OF SERUM FA
Page 3 and 4:
Table of contentsIndex of Figures .
Page 5 and 6:
Chapter Four - Attempts to find an
Page 7 and 8:
Index of figures1.1. Simplified sch
Page 9 and 10:
6.17. Longitudinal analysis of OPG
Page 11 and 12:
6.49. Longitudinal analysis of ColI
Page 13 and 14:
A.2. Complete list of BSALP correla
Page 15 and 16:
List of abbreviationsα-MEM. Alpha-
Page 17 and 18:
PCR. Polymerase chain reactionPer1
Page 19 and 20:
G2/M checkpoint. The point in the c
Page 21 and 22:
Species names13-lined ground squirr
Page 23 and 24:
Chapter One - Background and signif
Page 25 and 26:
1.3 Bone turnover is unbalanced dur
Page 27 and 28:
pro-apoptosis regulator BAX. An imp
Page 29 and 30:
low-dose administration of parathyr
Page 31 and 32:
decrease in bone trabecular thickne
Page 33 and 34:
experience periodic arousals in whi
Page 35 and 36:
ears must have evolved a mechanism
Page 37 and 38:
1.7.6 Hypothesis 2: OCN interacts w
Page 39 and 40:
ain, heart, and femoral muscle of h
Page 41 and 42:
Hypothesis 3a: Serum concentrations
Page 44:
were released. Behavior indicating
Page 47 and 48:
2.3 ResultsSerum activity of the bo
Page 49 and 50:
(p
Page 51 and 52:
post-hibernation sampling in the be
Page 53 and 54:
also unclear whether ucOCN may affe
Page 55 and 56:
atios of intact to fragmented OCN m
Page 57 and 58:
Table 2.3—Bone marker “seasonal
Page 59 and 60:
Table 2.7—Chemistry panel “seas
Page 61 and 62:
ABNormalized BSALPBSALP (U/L)CO N D
Page 63 and 64:
Total Calcium (mg/dL)A1.21.151.11.0
Page 65 and 66:
ABTotal OCN (ng/mL)150100500O N D J
Page 67 and 68:
Adiponectin (ng/mL)AB40003500300025
Page 69 and 70:
ABNormalized InsulinInsulin (μU/mL
Page 71 and 72:
surrounding and encompassing hibern
Page 73 and 74:
decreased from 788+30 ng/mL in preh
Page 75 and 76:
120-122]. Most small hibernators ar
Page 77 and 78: ears. Similarly, serum NPY increase
Page 79 and 80: Table 3.2—Serum factor longitudin
Page 81 and 82: Leptin (ng/mL)ABO N D J F M A M O N
Page 83 and 84: Norepinephrine (ng/mL)A807060504030
Page 85 and 86: IGF‐1 (ng/mL)AN D J F M A MB10009
Page 87 and 88: 2008. Samples were collected as des
Page 89 and 90: sampling points. It is possible to
Page 91 and 92: C‐Terminal PTH (Relative)AB504030
Page 93 and 94: Chapter Five — Serum from hiberna
Page 95 and 96: Synergy HT Multi-Detection Micropla
Page 97 and 98: gene specific primers and 12.5 μL
Page 99 and 100: involved in the reduced caspase-3/7
Page 101 and 102: hibernation cycles without problem
Page 103 and 104: filtered seasonal bear serum and fo
Page 105 and 106: which is dependent upon caspase-3 a
Page 107 and 108: Caspase‐3/7 ActivityO N D J F M A
Page 109 and 110: 6Caspase‐3/7 Activity54321**0Vehi
Page 111 and 112: ABCaspase‐3/7 Activity9876543210*
Page 113 and 114: eyond the G1/S checkpoint, thus ind
Page 115 and 116: ears, it is possible that tissue se
Page 117 and 118: PTH1R, RANKL, Runx2, Smurf1, TLR4,
Page 119 and 120: factors Runx2 and OCN also increase
Page 121 and 122: Table 6.3—Gene expression 3 hour
Page 123 and 124: Table 6.5—Gene expression 6 day d
Page 125 and 126: Cyclin D1 Gene ExpressionO N D J F
Page 127: Smurf1 Gene ExpressionO N D J F M A
Page 131 and 132: M‐CSF Gene ExpressionO N D J F M
Page 133 and 134: Per1 Gene ExpressionO N D J F M A M
Page 135 and 136: Akt Gene ExpressionO N D J F M A MM
Page 137 and 138: Bcl‐2 Gene ExpressionO N D J F M
Page 139 and 140: Cyclin D1 Gene ExpressionO N D J F
Page 141 and 142: OPN Gene ExpressionO N D J F M A MM
Page 143 and 144: 2PTH1R Gene Expression1.510.50O N D
Page 145 and 146: TLR4 Gene ExpressionO N D J F M A M
Page 147 and 148: Bcl‐2 Gene ExpressionO N D J F M
Page 149 and 150: OCN Gene ExpressionO N D J F M A MM
Page 151 and 152: PTH1R Gene ExpressionO N D J F M A
Page 153 and 154: TLR4 Gene ExpressionO N D J F M A M
Page 155 and 156: condition in renal patients in whic
Page 157 and 158: hibernation. This report brings to
Page 159 and 160: 14. Kaneps, A.J., S.M. Stover, and
Page 161 and 162: 41. Chowdhury, I., B. Tharakan, and
Page 163 and 164: 68. Ashe, M.C., et al., Bone geomet
Page 165 and 166: 95. Perrien, D.S., et al., Aging al
Page 167 and 168: 122. Lesser, R.W., et al., Renal re
Page 169 and 170: 151. Confavreux, C.B., R.L. Levine,
Page 171 and 172: 178. Toribara, T.Y., A.R. Terepka,
Page 173 and 174: 206. Luo, X.H., et al., Adiponectin
Page 175 and 176: 233. Florant, G.L., et al., Fat-cel
Page 177 and 178: 259. Bhasin, S., et al., Older men
Page 179 and 180:
284. Miura, M., et al., A crucial r
Page 181 and 182:
Appendix A—Complete tables of cor
Page 183 and 184:
Table A.5—Complete list of ionize
Page 185 and 186:
Table A.7—Complete list of adipon
Page 187 and 188:
Table A.12—Complete list of norep
show all

C - Michigan Technological University

Create successful ePaper yourself

Delete template?

Save as template?