C - Michigan Technological University

Runx2 Gene ExpressionO N D J F M A MMonth of Serum CollectionFigure 6.41—Longitudinal analysis of Runx2 gene expression in undifferentiated cellsstimulated for 24 hours in seasonal bear serumUndifferentiated MC3T3-E1 cells were cultured in seasonal bear serum for 24 hours, followedby real-time PCR quantification of relative gene expression. Each point represents the geneexpression response from one serum sample. Vertical lines represent transitions betweenactive and hibernating seasons. Samples are from 5 black bears in the 2007/08 season.Smurf1 Gene Expression21.510.50O N D J F M A MMonth of Serum CollectionFigure 6.42—Longitudinal analysis of Smurf1 gene expression in undifferentiated cellsstimulated for 24 hours in seasonal bear serumUndifferentiated MC3T3-E1 cells were cultured in seasonal bear serum for 24 hours, followedby real-time PCR quantification of relative gene expression. Each point represents the geneexpression response from one serum sample. Vertical lines represent transitions betweenactive and hibernating seasons. Samples are from 5 black bears in the 2007/08 season.122