C - Michigan Technological University

Ubiquitin Gene ExpressionO N D J F M A MMonth of Serum CollectionFigure 6.21—Longitudinal analysis of ubiquitin gene expression in differentiated cellsstimulated for 3 hours in seasonal bear serumDifferentiated MC3T3-E1 cells were cultured in seasonal bear serum for 3 hours, followed byreal-time PCR quantification of relative gene expression. Each point represents the geneexpression response from one serum sample. Vertical lines represent transitions betweenactive and hibernating seasons. Samples are from 2 black bears in the 2006/07 season.ADRβ2 Gene ExpressionO N D J F M A MMonth of Serum CollectionFigure 6.22—Longitudinal analysis of ADRβ2 gene expression in undifferentiated cellsstimulated for 24 hours in seasonal bear serumUndifferentiated MC3T3-E1 cells were cultured in seasonal bear serum for 24 hours, followedby real-time PCR quantification of relative gene expression. Each point represents the geneexpression response from one serum sample. Vertical lines represent transitions betweenactive and hibernating seasons. Samples are from 2 black bears in the 2006/07 season.112