C - Michigan Technological University

TLR4 Gene ExpressionO N D J F M A MMonth of Serum CollectionFigure 6.59—Longitudinal analysis of TLR4 gene expression in cells differentiated for 6 daysin seasonal bear serumMC3T3-E1 cells were differentiated in seasonal bear serum for 6 days, followed by real-timePCR quantification of relative gene expression. Each point represents the gene expressionresponse from one serum sample. Vertical lines represent transitions between active andhibernating seasons. Samples are from 2 black bears in the 2006/07 season.Ubiquitin Gene ExpressionO N D J F M A MMonth of Serum CollectionFigure 6.60—Longitudinal analysis of ubiquitin gene expression in cells differentiated for 6days in seasonal bear serumMC3T3-E1 cells were differentiated in seasonal bear serum for 6 days, followed by real-timePCR quantification of relative gene expression. Each point represents the gene expressionresponse from one serum sample. Vertical lines represent transitions between active andhibernating seasons. Samples are from 2 black bears in the 2006/07 season.131