C - Michigan Technological University

TLR4 Gene ExpressionO N D J F M A MMonth of Serum CollectionFigure 6.43—Longitudinal analysis of TLR4 gene expression in undifferentiated cellsstimulated for 24 hours in seasonal bear serumUndifferentiated MC3T3-E1 cells were cultured in seasonal bear serum for 24 hours, followedby real-time PCR quantification of relative gene expression. Each point represents the geneexpression response from one serum sample. Vertical lines represent transitions betweenactive and hibernating seasons. Samples are from 2 black bears in the 2006/07 season.Ubiquitin Gene ExpressionO N D J F M A MMonth of Serum CollectionFigure 6.44—Longitudinal analysis of ubiquitin gene expression in undifferentiated cellsstimulated for 24 hours in seasonal bear serumUndifferentiated MC3T3-E1 cells were cultured in seasonal bear serum for 24 hours, followedby real-time PCR quantification of relative gene expression. Each point represents the geneexpression response from one serum sample. Vertical lines represent transitions betweenactive and hibernating seasons. Samples are from 5 black bears in the 2007/08 season.123