4(%3)3 - Ecole nationale supérieure de chimie de Montpellier

The second class is represented by Bovine Serum Albumin protein (BSA) whose aqueoussuspension was prepared at different concentrations and the absorbance at 276 nm was measured byUV-Visible spectroscopy to obtain a calibration graph (Figure – 3.3). As compared to the randomstructure of PEG chains in aqueous medium, BSA bears a much compact and well defined structure in theform of a prolate ellipsoid (L a = L b < L c ) as measured by transient electric birefringence 26 (Figure – 3.4a,b).It can be assumed that while passing through the membrane, the structural integrity of the protein willbe maintained.Concentration (g.l -1 )1,210,80,60,40,20y = 0,6603xR² = 0,99870 0,5 1 1,5 2Absorbance (276 nm)Figure – 3.3: Absorbance vs. concentration calibration graph obtained for BSA protein using UltraViolet – VisibleSpectroscopyFigure – 3.4: 3-Dimenstional structure of Bovine Serum Albumin and it apparent shape as a prolate ellipsoid or aspheroid.78