III International Conference

PP-I-28For foam-like ceramics andsintered clay (keramzit), the synthesisFig. 2of filamentous carbon on the surfaceresulted in preparation of the effectiveadsorbents for immobilization ofinvertase-active membranes and nongrowingcells of baker yeast. Thebiocatalyst retained ∼50% of the initial2activity for >2 months storage atambient temperature (fig. 2, curve 2).1In spite of that, immobilization of yeastmembranes on graphite-like carbonlayer provided the less activity, and thebiocatalyst has lost 80% initial invertase activity for ∼2 months storage (fig. 2, curve 1) probablydue to hydrophobicity of the layer [2].For adsorptive immobilization of non-growing bacteria Arthrobacter nicotianae thatcatalyzed the glucose isomerization, the CFC-layer was synthesized on bellied vermiculiteand foam-like ceramics during hydrogen-propane-butane pyrolysis on the deposited Cocatalyst.It was found that non-growing bacteria A. nicotianae possessed a pure ability toadsorb on solid surfaces, and the adsorption value on CFC-coated ceramics was not exceed0,5 mg of dry cells per 1g of support at static conditions. Adsorptive immobilization ofA. nicotianae was carried out during its growth in the presence of nutrients and inorganicsupports when colonization of solid surface by bacteria occurred. The initial glucoseisomerase activity of the biocatalyst prepared on CFC-coated ceramics were equal to230 μmol/min/1 kg and retained constant after 12 hours of operation at 70 o C.From the experimental data, the inorganic supports under study are arranged in thefollowing order to increase enzymatic activity and stability of heterogeneous biocatalystprepared: supports coated by graphite-like carbon layer ≈ non-carbonized supports