New Pest Response Guidelines - aphis - US Department of Agriculture

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Survey Procedures Shipping Samples Call the laboratory prior to shipping the samples via overnight delivery service. Instructions and contact information are located in How to Submit Insect Specimens on page F-1 and Taxonomic Support for Surveys on page G-1. Collecting and Handling Samples and Specimens Adults—Moths captured alive adults should be transferred to a killing jar with ethyl acetate (killing agent). Adults will be quickly stunned but will be killed slowly. The body will remain limp (unless they are left in the killing jar overnight) in case spreading or removal of genitalia is needed (USDA, 1986). Alternatively, adults can be collected and placed in 95 percent alcohol. Collecting adults and storing them in dry ice will keep them in good shape in case further taxonomic studies are planned. Larvae and Pupae—For museum quality specimens, larvae and pupae extracted from plant material should be placed in a vial with ethanol at 70 to 80 percent and 5 percent glacial acetic acid. This combination of chemicals (referred to as acetic alcohol) aids in ethanol penetration and keeps specimen tissues relaxed (USDA, 1986). For surveys, glacial acetic acid is not necessary and 70 to 80 percent ethanol alone is all that is needed. Immature Stages in Plant Material—Place suspect material in a plastic bag and store in a cooler, but not frozen. A photograph should be taken in the field to document the plant materials original state. Data Collection Recording negative results in surveys is just as important as positive detections since it helps define an area of infestation. A system of data collection should include an efficient tracking system for suspect samples such that their status is known at various stages and laboratories in the confirmation process. If available, use pre-programmed hand-held units with GPS capability. Data collected during surveys should include the following: Date of survey Collector’s name and affiliation Full name of business, institution, or agency Full mailing address including country 4-14 Dendrolimus Pine Moths 12/2012-01
Survey Procedures Type of property (commercial nursery, hotel, natural field, residence) GPS coordinates of the host plant and property Host species and cultivar General conditions or any other relevant information Positive or negative results from specimen collection Whenever possible, record wind direction Time of capture (day or night) Cooperation with Other Surveys Other surveyors regularly sent to the field should be trained to recognize infestations of Dendrolimus moths. 12/2012-01 Dendrolimus Pine Moths 4-15
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United States Department of Agricul
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Dendrolimus Pine Moths Contents Con
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Dendrolimus Pine Moths Figures Figu
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Figure 4-3 Forest stand with glue b
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Dendrolimus Pine Moths Tables Table
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Dendrolimus Pine Moths Authors Revi
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Chapter 1 Introduction Contents Int
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Agricultural interests in other cou
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Authorities 4. Survey Procedures on
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! DANGER DANGEROUS indicates that p
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Cross-references to headings and ti
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Chapter 2 Contents Introduction Cla
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Table 2-1 Classification of Dendrol
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Ecological Range Pest Information D
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Pest Information Dendrolimus sibiri
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Pest Information Dendrolimus supera
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Potential Distribution Pest Informa
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Table 2-4 Reported Hosts Species fo
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Table 2-4 Reported Hosts Species fo
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Life Cycle Pest Information Dendrol
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Pest Information Larva After 16-25
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Pest Information Pupa Larvae spin h
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Dendrolimus superans Pest Informati
Page 47 and 48: Pest Information Egg Optimum temper
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Page 51 and 52: Pest Information Pupa Siberian silk
Page 53 and 54: Table 2-6 Daily Consumption (in g)
Page 55 and 56: Pest Information Dendrolimus supera
Page 57 and 58: Pest Information Dendrolimus sibiri
Page 59 and 60: Damage Pest Information The main da
Page 61 and 62: outbreaks of Dendrolimus spp. can m
Page 63 and 64: Pest Information Dendrolimus supera
Page 65 and 66: Chapter 3 Identification Contents I
Page 67 and 68: Identification Males are smaller an
Page 69 and 70: Figure 3-4 Pine-tree lappet larva.
Page 71 and 72: Identification Eggs The eggs are fo
Page 73 and 74: Identification Pupae Male pupae are
Page 75 and 76: Identification Figure 3-9 Adult Sib
Page 77 and 78: Identification Pupae The pupae of S
Page 79 and 80: Identification Similar Species In t
Page 81 and 82: Identification Figure 3-14 Larva of
Page 83 and 84: Identification Molecular tools have
Page 85 and 86: Chapter 4 Contents Introduction Sur
Page 87 and 88: Survey Procedures 5. Clearly mark t
Page 89 and 90: Monitoring Survey Survey Procedures
Page 91 and 92: Survey Procedures What To Look For
Page 93 and 94: Survey Procedures Figure 4-3 Forest
Page 95 and 96: Survey Procedures Dendrolimus punct
Page 97: Light Traps Survey Procedures Figur
Page 101 and 102: Chapter 5 Contents Introduction Reg
Page 103 and 104: Regulatory Procedures Tribal Govern
Page 105 and 106: Regulatory Procedures Establishing
Page 107 and 108: Chapter 6 Contents Introduction Con
Page 109 and 110: Cultural Control Pest population is
Page 111 and 112: Chemical Control Control Procedures
Page 113 and 114: Control Procedures Insect Growth Re
Page 115 and 116: Control Procedures Dendrolimus punc
Page 117 and 118: Control Procedures Dendrolimus sibi
Page 119 and 120: Control Procedures Dendrolimus pini
Page 121 and 122: Control Procedures Integrated Pest
Page 123 and 124: Chapter 7 Environmental Compliance
Page 125 and 126: Environmental Compliance Categorica
Page 127 and 128: Environmental Compliance Environmen
Page 129 and 130: Chapter 8 Pathways Contents Introdu
Page 131 and 132: Destinations Pathways When an actio
Page 133 and 134: Pathways Contaminated vehicles and
Page 135 and 136: Dendrolimus Pine Moths References U
Page 137 and 138: References Boldaruev, V. O. 1959. D
Page 139 and 140: References Dobrowolski, M. 2002. Ef
Page 141 and 142: References Gould, P., I. Wolwood, J
Page 143 and 144: outbreaks in eastern Siberia. Inter
Page 145 and 146: References Le-Cerf, F. 1932. Lepido
Page 147 and 148: References Malyshev, D. 1988. Durat
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References Endromidae, Saturnidae,
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References Shvidenko, A., S. Nilsso
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References Wang, C., J. Wu, and G.
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References Zhao, T. H., C. J. Chen,
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Appendix A Resources Use Appendix A
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Appendix B Forms index. Contents Us
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OMB Information According to the Pa
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Table B-1 Instructions for Completi
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PPQ 523 Emergency Action Notificati
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Appendix C How Contents to Submit I
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How to Submit Insect Specimens An a
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Appendix D Taxonomic Contents Backg
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Taxonomic Support for Surveys pleas
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USDA, APHIS, PPQ 105 Buckhout Lab P
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Data Entry Taxonomic Support for Su
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Appendix E Images Figure E-1 Field
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Images Figure E-3 Dendrolimus pini
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Appendix F Biological Control Table
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Sarcophaga tuberosa Pandellé Sarco
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Aphanistes bigutattus Grav Melis, 1
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Jackdaws (Corvus monedula) Chaffinc
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Nabis kurilensis Matsumura Coleopte
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Delomerista mandibularis Gravenhors
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Theronia atalantae Poda Theronia ja
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Dendrolimus sibiricus Cytoplasmic P
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Biological Control Table F-3 Report
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