Chemical and Functional Properties of Food Saccharides

© 2004 by CRC Press LLC
Mass Fraction
Eluent Pump Injector
0.08
0.06
0.04
0.02
0
Separation
system:
SEC
10 20 30 40 50 60
Re [nm]
LS
Visc
Mass
Flow
meter
Fraction
collector
0.08
0.06
0.04
0.02
FIGURE 22.6 Determination of excluded volume distribution (m_VeD) of starch polysaccharides
by SEC mass, light scattering, and viscosity triple detection, mass by refractive index
DRI (⎯∆⎯), scattering intensity as Rayleigh factor at Θ = 5° (⎯o⎯), specific viscosity via
differential pressure (ip) and inlet pressure (dp) (⎯□⎯). Normalized (area = 1.0) sphere
equivalent radii distribution (R eD) of SEC-separated wheat glucans, differential mass fraction
(⎯⎯), differential molar fractions (⎯⎯).
[ ] → [ ]
[ η]
⎡mL
⎤
i ⎢ ⎥
⎣ g ⎦
∝
[ η]
= K ⋅ M
mass _ ev( i)
eta _ spec( i)
a
i i
i=int_start…int_stop M lg( M ) raw_MWV: lg(M ) vs V
i i
0
Molar Fraction
i ret
i=int_start…int_stop [ η] → lg( [ η] ) eta_int → eta_int lg : lg( [ η]
) vs V
i i
i ret
(22.2b)
(22.2c)
(22.2d)
(22.2e)