ECOLOGICAL STUDIES ON TILE SYMBIOSIS OF TER ITOMYCES ...

3*le5 THE WECT ON SPORE GMINATION Or, COMUNDS APPEARING
SUITABIZ FM A SELECTIVE MIM
3*1*5ol Methods
It Is important that the selective medium does not affect the
gemination of Ternit=ces spores as vell as not affectina. its
mycelial growth. Spores and mycelia often have different sensitivities
to factoras such as Ingredients, in selective media and so high
concentrations of antimicrobial agents used In telectivo media that aro
non-inhibitory to mycelial growth might be Inhibitory to spores
1970), For this reason the spore gemination of Temitomyces was
tested on media containing the substances appearing suitable for
inclusion In the selective mediums
SWP, ensions of A=rVillus niner and PaecilMXces variotii and
conidial agglomerations of Term1tq%yqes in sterile distilled waterg
were pipetted alone and in combination on to plates of basic medium
W)
and selective media (see below)* If only one fungus was used
0,1 ml was pipettedl 2 fungi 0.5 =I each and 3 0.03 ml each*
The plates were Incubated at 290C and the number of fungi
developing counted*
13H (Appendix 1)
Candidate selective media (CSM)
CSM (1) = VI4 - glucose
+ cellophane overlays 0*01 Cl-I
Denlatet 0.01 Ul'I Murphy, 0.05 gl'I C"5040 5112.09
Oal al-I novoblocin
CSIf (21) w BM - alucose
nannitol
(10 ol-1)
(Tsao
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