ECOLOGICAL STUDIES ON TILE SYMBIOSIS OF TER ITOMYCES ...

not distort-the numbers as much as It might first appear tot as a
large number of species do not exhibit a frequency in the field
proportional to their sporulative capacity In artificial media,
cog. Trichoderma. - Thus the majority of fungi found on the ordinary
dilution plates out be judged to originate from spores,
An advantage Is given to rapidly growing fungif and slow
starting and slow growing fungi are likely to be suppressed
1951)e Warcup (1955a)
showed that nasidiomycates are particularly
(Garrott
selected against* The fast growing fungi are also favoured in the
&oil plate method, Garrett (1951)
considered this need not be too
serious provided final dilutions are chosen so that the number of
fungi/plate are not too highl thus reducing the Interference between
developing colonies through competition and antagonism. The ideal
number of colonies per plate to variou3ly given an 30-100 (Waksman
1944), 35-45 (Brierley, Jewson and Brierley 19,27). an average of 25
(Disby, James and Timonin 1933, Garrett 1951), this final figure being
found MtatiBt: iCallY Permissible by Jamex and Sutherland (1939)9
Howevers even wLth low numbers suppression does, occure
Those ftmgi whose propagules are attached to coarso particles
175-
that undergo a rapid sedimontation-or flotAtLon. will not-be Included In
the dilution, sarless but Ifornby (1969) found that. the contribution of
any-fungal material attachod to soil particles was Insignificant.
5,1,2 lXnICDS. r=ABLLSIUZVT OF MMODS TO DE U.
SED IN TIM
INVE STIGATION
There are a large. raimber of different, mothod, 0-- of Asolating micro-
erganis=,: rrom soll, and otlier, materials. ä. 4,. -,
Curl 19729 Park1noon 1973 and Warcup 19GO)o